本文選題：壬基酚　切入點(diǎn)：SD母鼠　出處：《毒理學(xué)雜志》2017年01期 　論文類型：期刊論文

【摘要】：目的探討母鼠(SD大鼠)孕期暴露壬基酚(NP)對(duì)孕中期及產(chǎn)后雌激素(E_2)、孕激素(P)以及血清NP水平和生產(chǎn)的影響。方法試驗(yàn)分為NP 50、100和200 mg/kg·d和玉米油對(duì)照4組。母鼠受孕第6~20 d灌胃染毒NP,分別在孕12 d,分娩后1 d處死母鼠,取血清,化學(xué)發(fā)光法檢測(cè)E_2、P水平,液相色譜法檢測(cè)NP含量,并觀察母鼠生產(chǎn)及產(chǎn)后哺乳的相關(guān)指標(biāo)。結(jié)果孕12 d,與對(duì)照組比較,低、中、高劑量組大鼠血清中NP、E_2、P及E_2/P差異有統(tǒng)計(jì)學(xué)意義(P0.01)。孕12 d,低、中和高劑量組大鼠血清中NP和E_2高于對(duì)照組、P低于對(duì)照組(P0.01)。分娩后1 d,與對(duì)照組比較,低、中、高劑量組母鼠血清中NP、E_2差異有統(tǒng)計(jì)學(xué)意義(分娩后1 d,低、中、高劑量組母鼠血清中NP、E_2均高于對(duì)照組,P0.01)。各染毒組母鼠未能正常生產(chǎn)和產(chǎn)后不哺乳數(shù)量均高于對(duì)照組,低劑量組和中劑量組仔鼠窩重均小于對(duì)照組(P0.05),與對(duì)照組比較,中劑量組和高劑量組的雌雄比例較大(P0.05)。結(jié)論孕期暴露NP,可降低孕鼠的體質(zhì)量,血清NP的含量隨暴露劑量增高而升高,干擾孕鼠體內(nèi)E_2、P水平,導(dǎo)致孕鼠不能正常生產(chǎn)和產(chǎn)后不哺乳,增加F1代仔鼠雌雄比例。
[Abstract]:Objective to investigate the effects of nonylphenol (NPN) exposure during pregnancy on the levels of serum NP and production of estradiol in the second trimester and postpartum of SD rats. Methods the experiment was divided into 4 groups: NP 50,100 and 200 mg/kg 路d and corn oil. NPs were administered intragastrically on the 6th day of gestation and killed on the 12th day of gestation and 1 day after delivery. Serum samples were collected. The levels of E _ 2P were detected by chemiluminescence method. NP content was detected by liquid chromatography, and the relative indexes of maternal production and postpartum lactation were observed. Results at the 12th day of pregnancy, compared with the control group, the serum levels were lower and lower. There was significant difference in serum NPP and ESP between high dose group and high dose group (P 0.01). At the 12th day of pregnancy, the levels of NP and ESP in the middle and high dose groups were higher than those in the control group (P < 0.01). 1 day after delivery, the levels of NP and E _ 2 in the serum of the rats in the control group were lower and lower than those in the control group. There was a statistically significant difference in serum NPD E2 between high dose group and high dose group (1 day after delivery, 1 day after delivery, 1 day after delivery, medium dose group, high dose group all higher than control group (P 0.01). The number of normal production and postpartum unlactation of each exposed group was higher than that of control group. Compared with the control group, the weight of litter in the low dose group and the middle dose group was lower than that in the control group. Compared with the control group, the proportion of female and male in the middle dose group and the high dose group was larger than that in the control group. Conclusion exposure to NPs during pregnancy can reduce the body weight of pregnant mice. The content of serum NP increased with the increase of exposure dose, which interfered with the level of E _ 2P in pregnant rats, which led to the abnormal production of pregnant mice and non-lactation of postpartum, and increased the ratio of male and female of F1 offspring.
【作者單位】： 遵義醫(yī)學(xué)院公共衛(wèi)生學(xué)院;遵義醫(yī)學(xué)院附屬醫(yī)院核醫(yī)學(xué)科;遵義醫(yī)學(xué)院附屬醫(yī)院胃腸外科;
【基金】：國(guó)家自然科學(xué)基金(81360439,81560527) 貴州省科技廳基金(黔科合LH字[2014]7543,[2015]7521號(hào),黔科合J字[2014]2177號(hào),[2014]2185號(hào)) 遵義醫(yī)學(xué)院招標(biāo)課題(2013F-68,2015F-784) 貴州省教育廳青年基金(黔教合KY字[2013]198) 遵義醫(yī)學(xué)院2015重點(diǎn)學(xué)科建設(shè)經(jīng)費(fèi) 貴州省衛(wèi)生廳基金(gzwkj20131127)
【分類號(hào)】：R114

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本文編號(hào)：1582373