本文選題：PC細(xì)胞　切入點(diǎn)：納米材料　出處：《中國(guó)職業(yè)醫(yī)學(xué)》2017年05期 　論文類型：期刊論文

【摘要】：目的研究納米氧化釹(Nano-Nd_2O_3)對(duì)大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞PC12(以下簡(jiǎn)稱"PC12細(xì)胞")的損傷作用。方法將對(duì)數(shù)生長(zhǎng)期的PC12細(xì)胞分為對(duì)照組和7個(gè)劑量組,對(duì)照組不予Nano-Nd_2O_3處理,各劑量組分別予終質(zhì)量濃度為3.13、6.25、12.50、25.00、50.00、75.00、100.00 mg/L的Nano-Nd_2O_3染毒24 h,選擇后續(xù)實(shí)驗(yàn)劑量。采用CCK-8法檢測(cè)3.13、6.25、12.50、25.00、50.00、75.00 mg/L Nano-Nd_2O_3組PC12細(xì)胞存活率,并采用酶標(biāo)法測(cè)定上述6組細(xì)胞的谷胱甘肽(GSH)水平和超氧化物歧化酶(SOD)活力,采用免疫印跡法檢測(cè)PC12細(xì)胞內(nèi)B細(xì)胞淋巴瘤/白血病-2(Bcl-2)、Bcl-2相關(guān)X蛋白(Bax)和天冬氨酸特異性半胱氨酸蛋白酶(Caspase)-3蛋白的表達(dá)。結(jié)果隨著Nano-Nd_2O_3染毒劑量的增加,PC12細(xì)胞存活率、GSH水平和Bcl-2/Bax比值均下降,均呈劑量-效應(yīng)關(guān)系(P0.01)。12.50、25.00、50.00和75.00 mg/L Nano-Nd_2O_3組PC12細(xì)胞的SOD活力均低于對(duì)照組(P0.05)。各劑量組PC12細(xì)胞Caspase-3/β-actin比值均高于對(duì)照組(P0.05),在0.00~25.00 mg/L Nano-Nd_2O_3劑量范圍,隨著Nano-Nd_2O_3染毒劑量的增加,PC12細(xì)胞的Caspase-3/β-actin比值逐漸上升,呈劑量-效應(yīng)關(guān)系(P0.01)。結(jié)論 Nano-Nd_2O_3可導(dǎo)致PC12細(xì)胞發(fā)生氧化應(yīng)激,降低Bcl-2/Bax比值,激活Caspase-3表達(dá),從而抑制PC12細(xì)胞的增殖,并呈劑量-效應(yīng)關(guān)系。
[Abstract]:Objective to study the damage of neodymium oxide Nano-Nd2O3 on adrenal pheochromocytoma cell line PC12 (PC12 cells). Methods PC12 cells in logarithmic growth phase were divided into control group and 7 dose groups, the control group was not treated with Nano-Nd_2O_3. Each dose group was exposed to Nano-Nd_2O_3 with final mass concentration of 3.136.25 ~ 12.50 ~ 25.00 mg/L for 24 h. The survival rate of PC12 cells in the Nano-Nd_2O_3 group was determined by CCK-8 method. The glutathione glutathione (GSH) level and the activity of superoxide dismutase (SOD) were measured by enzyme labeling method. Western blotting was used to detect the expression of Bcl-2 related X protein Baxand aspartate specific cysteine protease Caspase- 3 in PC12 cells. Results the survival rate of PC12 cells increased with the increase of the dose of Nano-Nd_2O_3. The level of GSH and the ratio of Bcl-2/Bax decreased, There was a dose-effect relationship between the SOD activity of PC12 cells in P0.01U, 12.50,25.00N50.00 and 75.00 mg/L Nano-Nd_2O_3 groups, and the ratio of Caspase-3/ 尾 -actin in PC12 cells in each dose group was higher than that in the control group (P0.05N). The Caspase-3/ 尾 -actin ratio of PC12 cells increased gradually with the increase of Nano-Nd_2O_3 exposure dose, and the ratio of Caspase-3/ 尾 -actin in PC12 cells was higher than that in the control group (P 0.05). Conclusion Nano-Nd_2O_3 can induce oxidative stress in PC12 cells, decrease the ratio of Bcl-2/Bax, activate the expression of Caspase-3 and inhibit the proliferation of PC12 cells in a dose-effect manner.
【作者單位】： 內(nèi)蒙古科技大學(xué)包頭醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)與法醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(81160341) 內(nèi)蒙古自治區(qū)研究生科研創(chuàng)新項(xiàng)目[S201610127(Y01)]
【分類號(hào)】：R114

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