本文關(guān)鍵詞： 亞砷酸鈉 膀胱上皮細胞 血管內(nèi)皮生長因子 抗氧化　出處：《環(huán)境與健康雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的探討抗氧化劑褪黑素(melatonin,MEL)、N-乙酰半胱氨酸(N-acetyl cysteine,NAC)、維生素C(Vitamin,VC)對砷誘導(dǎo)人正常膀胱上皮(SV-HUC-1)細胞血管內(nèi)皮生長因子(vascular endothelial growth factor,VEGF)表達的拮抗作用。方法將處于對數(shù)生長期的SV-HUC-1細胞暴露于含終濃度分別為0(對照)、0.5、1、2、4、8、10μmol/L亞砷酸鈉的F12K完全培養(yǎng)基染毒24 h,或者含終濃度分別為4、10μmol/L亞砷酸鈉的F12K完全培養(yǎng)基染毒0(對照)、4、12、24、48、72 h;聯(lián)合暴露組在加入含終濃度分為4μmol/L亞砷酸鈉的F12K完全培養(yǎng)基前30 min分別加入1%二甲基亞砜(DMSO)和抗氧化劑MEL、VC、NAC(終濃度分別為0.5、1、1 mmol/L),染毒24 h。分別采用Western blot法和RT-PCR法檢測SV-HUC-1細胞VEGF蛋白和mRNA的表達水平。結(jié)果 10μmol/L亞砷酸鈉染毒組SV-HUC-1細胞VEGF mRNA的表達水平高于對照組,差異有統(tǒng)計學(xué)意義(P0.05);且隨著亞砷酸鈉染毒劑量的升高,SV-HUC-1細胞VEGF mRNA的表達水平呈上升趨勢。與對照組比較,4μmol/L亞砷酸鈉染毒12、24、48 h及10μmol/L亞砷酸鈉染毒24和48 h后SV-HUC-1細胞VEGF mRNA的表達水平均升高,差異有統(tǒng)計學(xué)意義(P0.05);且隨著亞砷酸鈉染毒時間的延長,各劑量組SV-HUC-1細胞VEGF mRNA的表達水平均呈先上升后下降的趨勢。與對照組比較,4μmol/L亞砷酸鈉+DMSO染毒組SV-HUC-1細胞中VEGF蛋白的表達水平均增加,差異有統(tǒng)計學(xué)意義(P0.05)。與4μmol/L亞砷酸鈉+DMSO染毒組比較,亞砷酸鈉+NAC染毒組SV-HUC-1細胞中VEGF蛋白的表達水平較高,差異有統(tǒng)計學(xué)意義(P0.05);而亞砷酸鈉與MEL和VC聯(lián)合染毒組SV-HUC-1細胞中VEGF蛋白的表達水平無明顯改變。結(jié)論砷能誘導(dǎo)人正常膀胱上皮細胞VEGF表達增加,NAC能增加VEGF表達。
[Abstract]:Objective to investigate the antagonistic effect of the antioxidant melatonin (melatonin), N-acetyl cysteine (N-acetyl cysteine) on the expression of vascular endothelial growth factor endothelial growth factor (VEGF) in human normal bladder epithelial cell line SV-HUC-1 induced by arsenic. Methods the SV-HUC-1 in logarithmic growth period was used to investigate the effect of melatonin on the expression of vascular endothelial growth factor (VEGF) in human normal bladder epithelial cell line SV-HUC-1. Methods the expression of vascular endothelial growth factor (VEGF) in human normal bladder epithelial cell line SV-HUC-1 was determined in logarithmic growth period. The cells were exposed to F12K complete medium containing 10 渭 mol/L sodium arsenite for 24 h or F12K containing final concentration of 4 10 渭 mol/L sodium arsenite for 0 h (control group 0 (control group 412124872 h); combined exposure group was exposed to F12K complete culture medium containing final concentration of 4 渭 mol/L sodium arsenite for 24 4872 h), and the combined exposure group was exposed to F12K complete medium containing 10 渭 mol/L sodium arsenite for 24 h. F12K complete medium with 4 渭 mol/L sodium arsenite was added with 1% dimethyl sulfoxide (DMSO) and antioxidant MELVCCONAC (0.5 渭 mol/L sodium arsenite) for 24 h. The expression of VEGF protein and mRNA in SV-HUC-1 cells were detected by Western blot method and RT-PCR assay, respectively, at the end concentration of 0.5 渭 mol / L ~ (-1) mmol-1 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1), respectively. Results the expression of VEGF mRNA in SV-HUC-1 cells exposed to 10 渭 mol/L sodium arsenite was higher than that in control group. The expression of VEGF mRNA in SV-HUC-1 cells increased with the increase of sodium arsenite dose. Compared with the control group, SV-HUC-1 cells were treated with 4 渭 mol/L sodium arsenite for 24 h and 10 渭 mol/L for 24 h and 48 h for 24 h and 48 h respectively. The expression level of VEGF mRNA was increased. The difference was statistically significant (P 0.05), and with the prolongation of sodium arsenite exposure time, The expression of VEGF mRNA in SV-HUC-1 cells increased at first and then decreased in each dose group. Compared with the control group, the expression of VEGF protein in SV-HUC-1 cells exposed to 4 渭 mol/L sodium arsenite DMSO was higher than that in control group. Compared with 4 渭 mol/L sodium arsenite DMSO group, the expression of VEGF protein was higher in SV-HUC-1 cells treated with sodium arsenite NAC than that in 4 渭 mol/L sodium arsenite DMSO group. The expression of VEGF protein in SV-HUC-1 cells exposed to sodium arsenite combined with MEL and VC did not change significantly. Conclusion arsenic can induce the expression of VEGF in human normal bladder epithelial cells and increase the expression of VEGF.
【作者單位】： 中國醫(yī)科大學(xué)公共衛(wèi)生學(xué)院地球化學(xué)性疾病研究室 "砷生物學(xué)作用評價及砷中毒防治"遼寧省重點實驗室;
【基金】：國家科學(xué)自然基金(81072244)
【分類號】：R114
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本文編號：1512021