發(fā)布時(shí)間：2018-02-11 23:38

  本文關(guān)鍵詞： 海參 海參皂苷 海參多糖 高尿酸血癥　出處：《中國(guó)海洋大學(xué)》2012年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：海參是重要的食物和藥物資源，含有多種活性物質(zhì)，具有抗腫瘤、抗真菌、抗氧化，免疫調(diào)節(jié)、降血脂等方面的作用。本實(shí)驗(yàn)，研究了海參及其活性成分海參多糖(sea cucumber polysaccharides, PSC)、海參皂苷(sea cucumber saponin,SSC)對(duì)飲食誘導(dǎo)以及肥胖伴隨的高尿酸血癥小鼠尿酸代謝的影響，并對(duì)其作用機(jī)制進(jìn)行初步探討，主要內(nèi)容包括以下幾部分： 1．喂食小鼠酵母浸粉建立飲食誘導(dǎo)的高尿酸血癥小鼠模型，研究了海參皂苷和除皂苷殘余物對(duì)高尿酸血癥的影響，結(jié)果發(fā)現(xiàn)兩者均具有良好的改善酵母浸粉致高尿酸血癥的作用。喂養(yǎng)14天后，海參皂苷低、高劑量組血清尿酸(Uric Acid，UA)水平分別較對(duì)照組降低53.1%(P 0.01)與55.6%(P 0.01)，除皂苷殘余物組在第9天時(shí)可顯著降低尿酸水平，但在14天時(shí)無(wú)顯著性差異。進(jìn)一步研究發(fā)現(xiàn)，海參皂苷及其除皂苷殘余物抑制小鼠血清尿酸升高與肝臟尿酸合成代謝關(guān)鍵酶黃嘌呤氧化酶(Xanthine Oxidase，XOD)和腺苷脫氨酶(Adenosine Deaminase，ADA)的活性受到抑制明顯相關(guān)，該實(shí)驗(yàn)結(jié)果同時(shí)證明，海參中含有的抑制血中尿酸水平升高的活性物質(zhì)除了皂苷外，還有其他活性成分存在。 2．根據(jù)上述實(shí)驗(yàn)結(jié)果，本研究從海參除皂苷殘余物中提取了海參多糖，進(jìn)一步比較研究了整海參、海參皂苷、海參多糖對(duì)酵母浸粉誘導(dǎo)的高尿酸血癥小鼠的尿酸代謝的影響，，并同時(shí)比較了菲律賓刺參(Pearsonothuria graeffei，Pg)、仿刺參(Apostichopus japonicus，Aj)、冰島刺參(Cucumaria frondosa，Cf)和美國(guó)肉參(Isostichopus badionotus，Ib)對(duì)高尿酸血癥的影響的差異，并從基因轉(zhuǎn)錄調(diào)節(jié)水平探討其作用機(jī)理。結(jié)果表明，海參皂苷和海參多糖以及四種海參均對(duì)酵母浸粉誘導(dǎo)的高尿酸血癥有明顯的改善作用。喂養(yǎng)14天后，各組尿酸水平均顯著下降，下降幅度分別是皂苷組14.8%(P 0.05)；多糖組20.9%(P 0.01)；菲律賓刺參組16.1%(P 0.05)、仿刺參組20.1%(P 0.01)、冰島刺參組12.2%(P 0.05)和美國(guó)肉參組25.3%(P 0.01)。各組均可以顯著抑制肝臟XOD和ADA的活性，同時(shí)海參皂苷和海參多糖還可以下降XOD和ADA mRNA表達(dá)，下調(diào)腎臟葡萄糖轉(zhuǎn)運(yùn)體9(GLUT9) mRNA表達(dá)，抑制尿酸的重吸收，上調(diào)腎臟有機(jī)陽(yáng)離子和肉毒堿轉(zhuǎn)運(yùn)體(OCTs和OCTNs) mRNA表達(dá)，改善腎功能。 3．根據(jù)上訴實(shí)驗(yàn)結(jié)果，本文進(jìn)一步研究了海參皂苷單體EA對(duì)肥胖伴隨的高尿酸血癥小鼠(db/db小鼠)的尿酸代謝和腎功能的影響，并通過(guò)基因轉(zhuǎn)錄調(diào)節(jié)水平和顯微結(jié)構(gòu)觀察探討其機(jī)理。結(jié)果發(fā)現(xiàn)，海參皂苷單體EA可顯著降低db/db小鼠的血清尿酸水平，分別降低了18.1%(P 0.05)和12.7%(P 0.05)，但并不降低肝臟XOD和ADA的活性和mRNA表達(dá)。EA可上調(diào)腎臟有機(jī)陰離子轉(zhuǎn)運(yùn)體1(OAT1)mRNA表達(dá)，促進(jìn)尿酸的排泄，上調(diào)腎臟OCTs和OCTNs的mRNA表達(dá)，改善腎功能。光鏡和電鏡的觀察結(jié)果也顯示EA可恢復(fù)腎臟結(jié)構(gòu)，保護(hù)腎臟，這與基因轉(zhuǎn)錄調(diào)節(jié)水平得到的結(jié)論相一致。 綜上所述，本文研究了海參及海參活性成分對(duì)飲食誘導(dǎo)的高尿酸血癥和肥胖伴隨的高尿酸血癥動(dòng)物尿酸代謝的影響，并初步探討了其作用機(jī)制，為海參的綜合利用提供了科學(xué)依據(jù)。
[Abstract]:The sea cucumber is an important food and drug resource, contains a variety of active substances with antitumor, antifungal, antioxidant, immunomodulatory, lowering lipid. In this experiment, the sea cucumber and its active constituents of sea cucumber polysaccharide (sea cucumber polysaccharides PSC (sea cucumber), holothurin saponin, SSC) on diet and the effect of induced hyperuricemia mice uric acid metabolism in obesity, and to explore its mechanism, the main contents include the following parts:
1. mice fed yeast extract to establish hyperuricemia mouse model of diet induced, studied and effects of saponins of sea cucumber saponin in residue of hyperuricemia, found that both has good improvement of yeast extract powder induced hyperuricemia effect. 14 days later, holothurin low, high dose group of serum uric acid (Uric Acid, UA) level compared with the control group decreased 53.1% (P 0.01) and 55.6% (P 0.01), in addition to saponin residue group can significantly reduce the level of uric acid in ninth days, but on the 14 day there was no significant difference. Further studies showed that the sea cucumber saponins and its saponin residue in mice with elevated serum uric acid inhibition of key enzyme of liver xanthine and uric acid anabolic enzymes (Xanthine Oxidase, XOD) and adenosine deaminase (Adenosine Deaminase, ADA) significantly inhibited the activity of the related experimental results also proved that the inhibition of blood contained in sea cucumber In addition to the saponins, the active substance in the middle uric acid level also exists in other active components.
2. according to the above results, this study from the sea cucumber saponins except residue extracted from sea cucumber polysaccharide, further comparative study of the effects of saponins of sea cucumber, sea cucumber, sea cucumber polysaccharide on uric acid metabolism in yeast extract induced hyperuricemia mice, and compared the Philippines sea cucumber (Pearsonothuria graeffei, Pg), sea cucumber (Apostichopus japonicus, Aj), sea cucumber (Cucumaria frondosa, Cf Iceland) and American ginseng (Isostichopus badionotus, Ib meat) influence on hyperuricemia, and from the gene transcription level to explore its mechanism. The results showed that the sea cucumber saponin and polysaccharide and four kinds of sea cucumber on yeast extract induced with hyperuricemia obvious improvement. 14 days later, the level of uric acid levels were significantly decreased, decreased saponin group 14.8% (P 0.05); polysaccharide group 20.9% (P 0.01); Philippines sea group 1 6.1% (P 0.05), Apostichopus japonicus group 20.1% (P 0.01), Iceland sea cucumber group 12.2% (P 0.05) and American ginseng meat group 25.3% (P 0.01). Each group can significantly inhibit the liver XOD and ADA activity, while the sea cucumber saponin and polysaccharides can also decrease XOD and ADA expression of mRNA and down-regulation of renal the dirty glucose transporter 9 (GLUT9) mRNA expression, inhibiting uric acid reabsorption and increased renal organic cation and carnitine transporters (OCTs and OCTNs) mRNA expression, improve renal function.
3. according to the experimental results, this paper further studies the sea cucumber saponin monomer EA on obesity with hyperuricemia mice (db/db mice) influence the uric acid metabolism and renal function, and through the regulation of gene transcription level and explore the mechanism of microstructure observation. The results showed that the sea cucumber saponin monomer EA can significantly decrease the level of serum uric acid in db/db mice the decreased 18.1% (P 0.05) and 12.7% (P 0.05), but did not reduce the liver XOD and ADA activity and mRNA expression of.EA can increase the renal organic anion transporter 1 (OAT1) mRNA expression, promote the excretion of uric acid, the upregulation of the expression of kidney OCTs and OCTNs mRNA, improve renal function. Results the observation of light and electron microscopy also showed that EA can restore the renal structure, protect the kidney, which is consistent with the level of gene transcription regulation conclusion.
In conclusion, the effects of sea cucumber and sea cucumber active ingredients on uric acid metabolism in hyperuricemia and obesity accompanied by hyperuricemia in animals were studied, and its mechanism was preliminarily discussed, which provided a scientific basis for the comprehensive utilization of sea cucumber.

【學(xué)位授予單位】：中國(guó)海洋大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：TS254.1;R151

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 胡人杰,于蘇萍,姜卉,王士賢;刺參酸性粘多糖與可的松聯(lián)用方案對(duì)小鼠腫瘤的抑制作用[J];癌癥;1997年06期

2 王顏剛;閆勝利;苗志敏;;高尿酸血癥及痛風(fēng)中西醫(yī)結(jié)合研究進(jìn)展[J];青島大學(xué)醫(yī)學(xué)院學(xué)報(bào);2006年01期

3 肖楓,曾名勇,董士遠(yuǎn),劉尊英;海參膠原蛋白的研究進(jìn)展[J];水產(chǎn)科學(xué);2005年06期

4 尚雁君;郭躍偉;黃才國(guó);賈睿;許強(qiáng)芝;魏善建;焦炳華;張建榮;;中國(guó)南海海綿提取物renierol對(duì)黃嘌呤氧化酶的抑制作用[J];第二軍醫(yī)大學(xué)學(xué)報(bào);2006年01期

5 鄭志萍;黃幼霞;;小鼠高尿酸血癥模型的建立[J];海峽藥學(xué);2011年09期

6 唐天華,姜國(guó)勝,李新,張玉昆,任海泉;海源降糖膠囊的小鼠降糖實(shí)驗(yàn)研究[J];中國(guó)海洋藥物;1999年03期

7 鄒崢嶸,易楊華,張淑瑜,周大錚,湯海峰;海參皂苷研究進(jìn)展[J];中國(guó)海洋藥物;2004年01期

8 邵繼紅,徐耀初,莫寶慶,沈洪兵;痛風(fēng)與高尿酸血癥的流行病學(xué)研究進(jìn)展[J];疾病控制雜志;2004年02期

9 尚雁君;李醫(yī)明;蔣山好;朱大元;魏善建;焦炳華;黃才國(guó);;玄參中苯丙素苷Acteoside對(duì)小鼠高尿酸血癥的影響[J];解放軍藥學(xué)學(xué)報(bào);2006年01期

10 王楠;宋小駿;;皂苷生物活性的研究進(jìn)展[J];醫(yī)學(xué)研究生學(xué)報(bào);2007年02期

相關(guān)會(huì)議論文 前1條

1 胡曉倩;王玉明;任兵興;王靜鳳;薛長(zhǎng)湖;;海參皂苷對(duì)脂肪肝大鼠脂質(zhì)代謝的影響[A];2008年中國(guó)水產(chǎn)學(xué)會(huì)學(xué)術(shù)年會(huì)論文摘要集[C];2008年

相關(guān)博士學(xué)位論文 前1條

1 陳士國(guó);幾種海洋動(dòng)物酸性多糖的結(jié)構(gòu)和活性研究[D];中國(guó)海洋大學(xué);2010年

相關(guān)碩士學(xué)位論文 前3條

1 胡曉倩;海參皂苷對(duì)脂質(zhì)代謝的影響及其機(jī)制研究[D];中國(guó)海洋大學(xué);2010年

2 李曉林;海參蟲(chóng)草復(fù)劑對(duì)糖尿病大鼠腎臟保護(hù)作用及其機(jī)制的研究[D];中國(guó)海洋大學(xué);2010年

3 劉治東;革皮氏海參皂苷抗腫瘤活性及其作用機(jī)制的研究[D];中國(guó)海洋大學(xué);2009年

本文編號(hào)：1504274