本文關(guān)鍵詞： PM. WI-人胚肺細(xì)胞 基因差異表達(dá) 斑點(diǎn)雜交 TNF-α IL- IL- IL-　出處：《西安交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2015年06期 　論文類型：期刊論文

【摘要】：目的尋找與大氣污染致病相關(guān)的相關(guān)基因,為闡明大氣污染致病的生物學(xué)機(jī)制提供科學(xué)依據(jù)。方法采用mRNA斑點(diǎn)雜交鑒定技術(shù),克隆經(jīng)采暖季≥75μg/m3 PM2.5與非采暖季75μg/m3 PM2.5染毒的WI-38人胚肺細(xì)胞,分析其間的基因表達(dá)差異。放免法測(cè)定炎性因子TNF-α、IL-2、IL-6和IL-8。結(jié)果與對(duì)照組比較,PM2.5100μg/mL染毒24h后,WI-38人胚肺細(xì)胞細(xì)胞因子TNF-α、IL-6和IL-8明顯升高,IL-2明顯減低(P0.05)。不同濃度PM2.5處理的WI-38人胚肺細(xì)胞間差異表達(dá)的基因片段,可見48份基因樣本在350bp處出現(xiàn)清晰條帶;該48份基因樣本經(jīng)斑點(diǎn)雜交后,Tester cDNA雜交的48個(gè)斑點(diǎn)中,41份可見黑褐色斑點(diǎn),而同樣樣本與Driver cDNA雜交的該48份樣本均未見明顯顯色。結(jié)論 PM2.5能誘導(dǎo)WI-38人胚肺細(xì)胞產(chǎn)生炎性損傷,≥75μg/m3 PM2.5染毒的WI-38人胚肺細(xì)胞存在明顯的基因損傷。
[Abstract]:Objective to search for the related genes related to the pathogenesis of atmospheric pollution and to provide scientific basis for elucidating the biological mechanism of the pathogenicity of atmospheric pollution. Methods mRNA dot blot hybridization technique was used to identify the pathogenicity of atmospheric pollution. The lung cells of WI-38 embryos were cloned from 75 渭 g / m3 PM2.5 and 75 渭 g / m3 PM2.5 in heating season and non-heating season respectively. The difference of gene expression between them was analyzed. The levels of IL-6 and IL-8 were measured by radioimmunoassay. The results were compared with those of the control group. After 24 hours of exposure to PM2.5100 渭 g / mL, WI-38 human embryonic lung cell cytokines TNF- 偽, IL-6 and IL-8 were significantly increased. IL-2 significantly reduced the differentially expressed gene fragments of WI-38 human embryonic lung cells treated with different concentrations of PM2.5. 48 samples showed clear bands at 350 BP. After dot blot hybridization, 41 of the 48 spots of 48 gene samples by Tester cDNA hybridization showed dark brown spots. However, none of the 48 samples hybridized with Driver cDNA showed obvious color. Conclusion PM2.5 can induce inflammatory injury in WI-38 human embryonic lung cells. There was significant gene damage in WI-38 human embryonic lung cells exposed to 75 渭 g / m ~ 3 PM2.5.
【作者單位】： 西安交通大學(xué)醫(yī)學(xué)部;
【基金】：2013年國(guó)家級(jí)大學(xué)生創(chuàng)新訓(xùn)練項(xiàng)目(No.201310698066)~~
【分類號(hào)】：R122
【正文快照】： 西安市采暖季PM2.5污染濃度顯著高于非采暖季;PM2.5的污染加劇是西安市居民心血管系統(tǒng)和呼吸系統(tǒng)疾病的死亡率升高的原因之一。隨著我國(guó)大氣污染的日趨嚴(yán)重,霧霾范圍和程度日益加重,對(duì)PM2.5與疾病的發(fā)病率、死亡率關(guān)系研究,尤其是呼吸系統(tǒng)疾病及心血管疾病發(fā)病機(jī)制的研究越來

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