本文關(guān)鍵詞：乙酸鉛和氯化鎘暴露對小鼠睪丸支持細(xì)胞乳酸水平的影響　出處：《環(huán)境與健康雜志》2017年09期 　論文類型：期刊論文

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【摘要】：目的探究乙酸鉛、氯化鎘單獨或聯(lián)合染毒對小鼠睪丸支持細(xì)胞(15P-1細(xì)胞)乳酸(LD)水平的影響。方法將15P-1細(xì)胞分設(shè)對照(空白培養(yǎng)基)組和乙酸鉛(0.05~160μmol/L)單獨染毒組、氯化鎘(0.05~160μmol/L)單獨染毒組,染毒24 h后,測定細(xì)胞存活率。后續(xù)實驗分別設(shè)對照(空白培養(yǎng)基)組和乙酸鉛(1~60μmol/L)單獨染毒組、氯化鎘(0.5~30μmol/L)單獨染毒組及乙酸鉛(1、10、20μmol/L)+氯化鎘(0.5、5、10μmol/L)聯(lián)合染毒組,染毒24 h后,測定細(xì)胞內(nèi)葡萄糖攝取量、乳酸脫氫酶(LDH)活力以及細(xì)胞內(nèi)、外LD濃度和p H值。結(jié)果與對照組比較,2.5~160μmol/L乙酸鉛和2.5~160μmol/L氯化鎘分別單獨染毒組15P-1細(xì)胞存活率均降低(P0.05)。與對照組比較,0.5~10μmol/L氯化鎘染毒15P-1細(xì)胞內(nèi)的葡萄糖攝取量較高(P0.05)。與相同濃度乙酸鉛+氯化鎘染毒組比較,僅0.5μmol/L氯化鎘單獨染毒組細(xì)胞內(nèi)葡萄糖攝取增加(P0.05)。與對照組比較,1、10μmol/L乙酸鉛染毒、各濃度氯化鎘染毒及10μmol/L乙酸鉛+5μmol/L氯化鎘染毒組15P-1細(xì)胞內(nèi)的LD濃度較高,而20~60μmol/L乙酸鉛染毒及20μmol/L乙酸鉛+10μmol/L氯化鎘染毒組15P-1細(xì)胞內(nèi)的LD濃度較低(P0.05);與對照組比較,1~30μmol/L乙酸鉛染毒及15、30μmol/L氯化鎘染毒15P-1細(xì)胞外的LD濃度均較低,而0.5、5μmol/L氯化鎘染毒及1μmol/L乙酸鉛+0.5μmol/L氯化鎘染毒組15P-1細(xì)胞外的LD濃度較高(P0.05)。與對照組比較,10~60μmol/L乙酸鉛染毒及5~15μmol/L氯化鎘染毒15P-1細(xì)胞內(nèi)的p H值較低(P0.05)。與對照組比較,20~60μmol/L乙酸鉛染毒及各濃度氯化鎘染毒15P-1細(xì)胞外的p H值較高(P0.05)。與對照組比較,各濃度乙酸鉛+氯化鎘染毒組15P-1細(xì)胞內(nèi)、外的p H值均較高(P0.05)。與相同濃度乙酸鉛+氯化鎘染毒組比較,1、10、20μmol/L乙酸鉛單獨染毒組及0.5、5、10μmol/L氯化鎘染毒組細(xì)胞內(nèi)p H值較高,而1、10、20μmol/L乙酸鉛單獨染毒組及0.5、10μmol/L氯化鎘單獨染毒組細(xì)胞外p H值較高(P0.05)。與對照組比較,各濃度乙酸鉛染毒15P-1細(xì)胞內(nèi)的LDH活力較低,而各濃度氯化鎘染毒15P-1細(xì)胞內(nèi)的LDH活力較高(P0.05)。與對照組比較,各濃度乙酸鉛+氯化鎘染毒組15P-1細(xì)胞內(nèi)LDH活力均較高(P0.05)。與相同濃度乙酸鉛+氯化鎘染毒組比較,1、10、20μmol/L乙酸鉛單獨染毒組及5、10μmol/L氯化鎘單獨染毒組細(xì)胞內(nèi)的LDH活力較高(P0.05)。結(jié)論乙酸鉛對生精系統(tǒng)的損傷可能通過降低細(xì)胞內(nèi)LDH活力進(jìn)而抑制細(xì)胞內(nèi)乳酸生成來實現(xiàn),而氯化鎘則可能通過抑制細(xì)胞內(nèi)LD轉(zhuǎn)運(yùn)到細(xì)胞外供給精子發(fā)生需要的能量來實現(xiàn)。
[Abstract]:Objective to explore lead acetate. Effects of cadmium chloride alone or in combination on LDL-lactic acid levels in mouse testicular Sertoli cells (Sertoli cells 15P-1). Methods 15P-1 cells were divided into control (blank medium) group and lead acetate (Pb) acetate group. 0. 05 渭 mol / L) alone. The cadmium chloride group (0.05 渭 mol / L) was exposed to cadmium chloride alone for 24 h. The cell survival rate was measured. The following experiments were divided into two groups: control group (blank medium) and lead acetate group (60 渭 mol / L) alone. Cadmium chloride (0.5 ~ 30 渭 mol 路L ~ (-1)) and lead acetate (10 ~ 10 ~ (20 渭 mol / L)) and cadmium chloride (0.5 ~ 5 渭 mol 路L ~ (-1)) combined with 10 渭 mol 路L ~ (-1 / L) group. After exposure for 24 h, the intracellular glucose intake, the activity of lactate dehydrogenase (LDH), the intracellular and extracellular LD concentration and pH value were measured, and the results were compared with those in the control group. The survival rate of 15P-1 cells in 2.5 ~ 160 渭 mol/L lead acetate group and 2.5 ~ 160 渭 mol/L cadmium chloride group was significantly lower than that in control group. The glucose uptake in 15P-1 cells exposed to 0.5 渭 mol/L cadmium chloride was higher than that in the same concentration of lead acetate and cadmium chloride group. Only 0.5 渭 mol/L cadmium chloride alone increased the intracellular glucose uptake of P0.05G, and compared with the control group, 10 渭 mol/L lead acetate was exposed to lead acetate. LD concentrations in 15P-1 cells were higher in cadmium chloride and 10 渭 mol/L lead acetate (5 渭 mol/L) groups. The LD concentration in 15P-1 cells of 20 渭 mol/L lead acetate and 20 渭 mol/L lead acetate 10 渭 mol/L cadmium chloride group was lower than that of 20 渭 mol/L lead acetate group. Compared with the control group, the concentration of LD in 15P-1 cells exposed to 30 渭 mol/L lead acetate and 1530 渭 mol/L cadmium chloride was lower than that in the control group. The LD concentration in 15P-1 cells of 5 渭 mol/L cadmium chloride group and 1 渭 mol/L lead acetate 0.5 渭 mol/L cadmium chloride group was higher than that of control group. The pH value of 15P-1 cells exposed to 10 ~ 60 渭 mol/L lead acetate and 5 ~ 15 渭 mol/L cadmium chloride was lower than that of control group. The pH value of 15P-1 cells exposed to 20 ~ 60 渭 mol/L lead acetate and cadmium chloride was higher than that of control group. Compared with the same concentration of lead acetate cadmium chloride group, the pH value of 15 P-1 cells was higher than that of the same concentration lead acetate cadmium chloride group. The intracellular pH value of 20 渭 mol/L lead acetate alone group and 0.5 渭 mol/L 10 渭 mol/L cadmium chloride group was higher than that of 1 渭 mol/L group. The extracellular pH value of 20 渭 mol/L lead acetate alone group and 0.5 渭 mol/L 10 渭 mol/L cadmium chloride group was higher than that of control group. The LDH activity in 15P-1 cells exposed to lead acetate was lower than that in the control group, while the LDH activity in 15P-1 cells exposed to cadmium chloride was higher than that in the control group. The activity of LDH in 15P-1 cells was higher in each concentration of lead acetate and cadmium chloride group than that in the same concentration of lead acetate and cadmium chloride group. 20 渭 mol/L lead acetate alone and 5. The intracellular LDH activity of 10 渭 mol/L cadmium chloride alone group was higher than that of P0.05 group. Conclusion the damage of lead acetate to spermatogenic system may be achieved by reducing the activity of LDH and inhibiting the production of lactic acid. Cadmium chloride may be achieved by inhibiting intracellular LD transport to the extracellular supply of energy needed for spermatogenesis.
【作者單位】： 武漢大學(xué)健康學(xué)院;
【基金】：國家博士后科學(xué)基金(2015M582279) 國家自然科學(xué)基金(81773471)
【分類號】：R114
【正文快照】： 環(huán)境重金屬鉛和鎘進(jìn)入機(jī)體后與組織器官有較高的親和性,且蓄積后不易排除。近年來,關(guān)于鉛、鎘暴露導(dǎo)致的男性不育等問題引起了廣泛的關(guān)注[1],但其作用機(jī)制仍不十分明確。睪丸是自然狀態(tài)下相對缺氧器官,睪丸支持細(xì)胞(Sertoli cell,SC)可通過糖酵解作用生成乳酸,同時,為生精細(xì)胞

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