發(fā)布時(shí)間：2019-08-13 08:46

【摘要】：目的觀察糖皮質(zhì)激素對(duì)小膠質(zhì)細(xì)胞內(nèi)鈣的影響并初步探討其機(jī)制。方法體外培養(yǎng)神經(jīng)小膠質(zhì)細(xì)胞株BV-2,使用Fluo3-AM作為鈣熒光染料,激光共聚焦顯微鏡實(shí)時(shí)掃描觀察氫化可的松處理后BV-2細(xì)胞內(nèi)鈣濃度的動(dòng)態(tài)變化情況。結(jié)果氫化可的松和陽(yáng)性對(duì)照藥尼古丁均能顯著升高BV-2細(xì)胞內(nèi)鈣水平(P0.05);實(shí)時(shí)掃描顯示氫化可的松即刻引起細(xì)胞內(nèi)鈣升高,15 s左右達(dá)到峰值,持續(xù)約10 s后開(kāi)始下降,200 s左右恢復(fù)至基態(tài),并且這一作用顯示出與尼古丁升高細(xì)胞內(nèi)鈣效應(yīng)的一致性。糖皮質(zhì)激素受體拮抗劑RU486不能取消氫化可的松升高BV-2細(xì)胞內(nèi)鈣的效應(yīng)(P0.05);而α7煙堿型乙酰膽堿受體(α7nAChR)阻斷劑甲基牛扁亭堿(MLA)可以拮抗氫化可的松升高BV-2細(xì)胞內(nèi)鈣的效應(yīng)(P0.05)。結(jié)論氫化可的松通過(guò)影響α7nAChR升高小膠質(zhì)細(xì)胞內(nèi)鈣水平,這一作用不但證實(shí)了糖皮質(zhì)激素的非基因組效應(yīng),同時(shí)提示糖皮質(zhì)激素可能作為α7nAChR的內(nèi)源性配體。
[Abstract]:Objective To observe the effect of glucocorticoid on calcium in microglia and to explore its mechanism. Methods The dynamic changes of calcium concentration in BV-2 cells treated with hydrocortisone were observed by using Fluo3-AM as a calcium fluorescent dye and a laser confocal microscope in vitro. Results The levels of calcium in the cells of BV-2 were significantly increased in both hydrocortisone and positive control (P0.05). The real-time scanning showed that the calcium in the cells increased immediately, the peak value of about 15 s reached the peak at about 15 s, the decrease after about 10 s, and the recovery to the ground state at about 200 s. And this effect shows the consistency of the calcium effect with the nicotine-increasing cells. Glucocorticoid receptor antagonist RU486 could not cancel the effect of hydrocortisone on calcium in BV-2 cells (P0.05); while the 7niacin-type B-acetylcholine receptor (p7 nAChR) blocking agent, MLA, could antagonize the effect of hydrocortisone on calcium in BV-2 cells (P0.05). Conclusion Hydrocortisone has not only confirmed the non-genomic effect of the glucocorticoid, but also suggested that the glucocorticoid may be the endogenous ligand of 7 nAChR.
【作者單位】： 廣州醫(yī)科大學(xué)藥學(xué)院藥理教研室;
【基金】：廣東省科技計(jì)劃資助項(xiàng)目(No 2013B022000096) 廣東省自然科學(xué)基金資助項(xiàng)目(No 2016A030313568)
【分類(lèi)號(hào)】：R96

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