【摘要】：內生真菌已經成為發(fā)現(xiàn)結構新穎、活性顯著的次級代謝產物的重要來源,紅樹林內生真菌的代謝產物中發(fā)現(xiàn)了許多結構多樣的化合物,引起了人們的廣泛重視。采用硅膠柱色譜、Sephadex LH-20凝膠柱色譜、以及制備薄層色譜等方法從南海紅樹林植物桐花樹內生雜色擬盤多毛孢真菌大米發(fā)酵產物中的乙酸乙酯提取物中得到4個代謝產物,其中1個新氯代二苯甲酮衍生物pestalachloride E(2),2個已知的二苯甲酮衍生物pestalachlorides C(1)和B(3),1個已知的蒽醌化合物,大黃素甲醚physcion(4)。新化合物通過一維和二維核磁共振譜鑒定,并通過高分辨電噴霧質譜確認。細胞毒實驗結果表明化合物1顯示良好的抗腫瘤活性,IC50在2.8~82.4μmol/L區(qū)間�；衔�3顯示具有抗炎活性,對環(huán)氧合酶-2的抑制活性為IC50 2.4μmol/L。
[Abstract]:Endophytic fungi have become an important source of the secondary metabolites with novel structure and active activity, and many compounds with various structures are found in the metabolites of the mangrove endophytic fungi. adopting silica gel column chromatography, Sephadex LH-20 gel column chromatography, and preparing a thin-layer chromatography to obtain 4 metabolites from the ethyl acetate extract in the rice fermentation product of the endogenous variegated polychaete chrysosporium in the mangrove plant of the South China Sea, One of the new chlorobenzophenone derivatives, pestalachloride E (2), two known benzophenone derivatives, pestalachlorides C (1) and B (3),1 known dibenzo compound, emodin methyl ether phycion (4). The new compound was identified by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and confirmed by high-resolution electrospray mass spectrometry. The results of the cytotoxicity test show that the compound 1 shows good anti-tumor activity and the IC50 is in the range of 2.8-82.4. m Compound 3 showed an anti-inflammatory activity and an inhibitory activity on cyclooxygenase-2 was IC50 of 2.4. m u.mol/ L.
【作者單位】： 中國科學院南海海洋研究所廣東省海洋藥物重點實驗室;中國科學院大學;廣州生物醫(yī)藥與健康研究院;南海生物資源開發(fā)與利用協(xié)同創(chuàng)新中心;
【分類號】：R915

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