【摘要】：目的:建立快速定量分析卡維地洛、厄貝沙坦及馬來酸噻嗎洛爾制劑及血清中含量的荷移-紫外分光光度法。方法:以紫外分光光度法為手段,研究卡維地洛、厄貝沙坦和馬來酸噻嗎洛爾與適宜的電子受體試劑之間的電荷轉移反應,優(yōu)化并確立最佳反應條件。結果:(1)卡維地洛與茜素電荷轉移反應最佳反應條件是:在室溫下,放置30min,形成1:1型穩(wěn)定的紅褐色荷移絡合物,最大吸收峰紅移至542nm,卡維地洛的濃度在5-180mg/L范圍內(nèi)線性良好,相關系數(shù)是r=0.9999。用確立的方法測定膠囊、片劑中卡維地洛含量回收率在99.4-101.0%之間,血清中卡維地洛回收率在101.13-101.35%。(2)厄貝沙坦與氯冉酸電荷轉移反應最佳反應條件:在室溫下即刻反應,生成了1:1型在穩(wěn)定的紅色荷移絡合物,最大吸收峰在520nm,荷移絡合物的線性范圍是10-800mg/L。藥物制劑的回收率在100.3-101.6%之間,血清中藥物回收率在99.7-100.05%。(3)馬來酸噻嗎洛爾與TCNQ電荷轉移反應:需要在50℃下反應60min,生成的絡合物最大吸收峰在842nm,藥物濃度在1-25mg/L范圍內(nèi)符合比爾定律。用擬定方法測定片劑、滴眼劑中噻嗎洛爾含量,回收率在樣回收率在100.4-102.2%,RSD為1.10-3.54%,血清中馬來酸噻嗎洛爾回收率測定結果是100.9-101.5%。結論:三種基于電荷轉移反應的分光光度法,簡便、快速、準確,對藥物含量測定結果令人滿意;對于質(zhì)量控制實驗室常規(guī)應用具有實用價值。
[Abstract]:Objective: To establish a rapid and quantitative analysis of the content of carvedilol, irbesartan and the metoprolol maleate and the determination of the content of the serum. Methods: The charge transfer reaction between carvedilol, irbesartan and dimaleate and appropriate electron acceptor reagent was studied by means of ultraviolet spectrophotometry, and the optimum reaction conditions were established. Results: (1) The best reaction conditions for the charge transfer reaction of carvedilol and fluorescein were as follows: at room temperature, the optimum reaction conditions were as follows: at room temperature for 30 min, a 1:1 type of stable red-brown charge-shift complex was formed, the maximum absorption peak was shifted to 542 nm, and the concentration of carvedilol was well within the range of 5-180 mg/ L, and the correlation coefficient was r = 0.9999. The content of carvedilol in the capsule was 99.4-101.0%, and the recovery of carvedilol in the serum was 101.13-101.35%. (2) The best reaction conditions for the charge transfer reaction of irbesartan and chlordecanoic acid are as follows: the reaction is carried out at room temperature, and a 1:1 type in the stable red charge-shift complex is generated, the maximum absorption peak is 520 nm, and the linear range of the charge-shift complex is 10-800mg/ L. The recovery rate of the pharmaceutical preparation is between 100.3 and 101.6%, and the recovery rate of the drug in the serum is 99.7-100.05%. (3) The charge transfer reaction between nolol maleate and TCNQ: it is necessary to react for 60 min at 50 & deg; C, the maximum absorption peak of the generated complex is 842 nm, and the drug concentration is in the range of 1-25 mg/ L to meet the Beer's law. The recovery was 100.4-102.2%, RSD was 1.10-3.54%, and the recovery was 100.9-101.5%. Conclusion: The three kinds of spectrophotometric method, which is based on the charge transfer reaction, are simple, rapid and accurate, and the result of the determination of the drug content is satisfactory; and it is of practical value to the routine application of the quality control laboratory.
【學位授予單位】：遵義醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R917

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