【摘要】：目的:建立快速定量分析卡維地洛、厄貝沙坦及馬來(lái)酸噻嗎洛爾制劑及血清中含量的荷移-紫外分光光度法。方法:以紫外分光光度法為手段,研究卡維地洛、厄貝沙坦和馬來(lái)酸噻嗎洛爾與適宜的電子受體試劑之間的電荷轉(zhuǎn)移反應(yīng),優(yōu)化并確立最佳反應(yīng)條件。結(jié)果:(1)卡維地洛與茜素電荷轉(zhuǎn)移反應(yīng)最佳反應(yīng)條件是:在室溫下,放置30min,形成1:1型穩(wěn)定的紅褐色荷移絡(luò)合物,最大吸收峰紅移至542nm,卡維地洛的濃度在5-180mg/L范圍內(nèi)線性良好,相關(guān)系數(shù)是r=0.9999。用確立的方法測(cè)定膠囊、片劑中卡維地洛含量回收率在99.4-101.0%之間,血清中卡維地洛回收率在101.13-101.35%。(2)厄貝沙坦與氯冉酸電荷轉(zhuǎn)移反應(yīng)最佳反應(yīng)條件:在室溫下即刻反應(yīng),生成了1:1型在穩(wěn)定的紅色荷移絡(luò)合物,最大吸收峰在520nm,荷移絡(luò)合物的線性范圍是10-800mg/L。藥物制劑的回收率在100.3-101.6%之間,血清中藥物回收率在99.7-100.05%。(3)馬來(lái)酸噻嗎洛爾與TCNQ電荷轉(zhuǎn)移反應(yīng):需要在50℃下反應(yīng)60min,生成的絡(luò)合物最大吸收峰在842nm,藥物濃度在1-25mg/L范圍內(nèi)符合比爾定律。用擬定方法測(cè)定片劑、滴眼劑中噻嗎洛爾含量,回收率在樣回收率在100.4-102.2%,RSD為1.10-3.54%,血清中馬來(lái)酸噻嗎洛爾回收率測(cè)定結(jié)果是100.9-101.5%。結(jié)論:三種基于電荷轉(zhuǎn)移反應(yīng)的分光光度法,簡(jiǎn)便、快速、準(zhǔn)確,對(duì)藥物含量測(cè)定結(jié)果令人滿意;對(duì)于質(zhì)量控制實(shí)驗(yàn)室常規(guī)應(yīng)用具有實(shí)用價(jià)值。
[Abstract]:Objective: To establish a rapid and quantitative analysis of the content of carvedilol, irbesartan and the metoprolol maleate and the determination of the content of the serum. Methods: The charge transfer reaction between carvedilol, irbesartan and dimaleate and appropriate electron acceptor reagent was studied by means of ultraviolet spectrophotometry, and the optimum reaction conditions were established. Results: (1) The best reaction conditions for the charge transfer reaction of carvedilol and fluorescein were as follows: at room temperature, the optimum reaction conditions were as follows: at room temperature for 30 min, a 1:1 type of stable red-brown charge-shift complex was formed, the maximum absorption peak was shifted to 542 nm, and the concentration of carvedilol was well within the range of 5-180 mg/ L, and the correlation coefficient was r = 0.9999. The content of carvedilol in the capsule was 99.4-101.0%, and the recovery of carvedilol in the serum was 101.13-101.35%. (2) The best reaction conditions for the charge transfer reaction of irbesartan and chlordecanoic acid are as follows: the reaction is carried out at room temperature, and a 1:1 type in the stable red charge-shift complex is generated, the maximum absorption peak is 520 nm, and the linear range of the charge-shift complex is 10-800mg/ L. The recovery rate of the pharmaceutical preparation is between 100.3 and 101.6%, and the recovery rate of the drug in the serum is 99.7-100.05%. (3) The charge transfer reaction between nolol maleate and TCNQ: it is necessary to react for 60 min at 50 & deg; C, the maximum absorption peak of the generated complex is 842 nm, and the drug concentration is in the range of 1-25 mg/ L to meet the Beer's law. The recovery was 100.4-102.2%, RSD was 1.10-3.54%, and the recovery was 100.9-101.5%. Conclusion: The three kinds of spectrophotometric method, which is based on the charge transfer reaction, are simple, rapid and accurate, and the result of the determination of the drug content is satisfactory; and it is of practical value to the routine application of the quality control laboratory.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R917

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 吳立明，，陳光巨，黃元河，劉若莊;芳香族多硝基類(lèi)化合物電子結(jié)構(gòu)的量子化學(xué)研究[J];北京師范大學(xué)學(xué)報(bào)(自然科學(xué)版);1995年03期

2 趙鳳林,周彪,徐變珍;電荷轉(zhuǎn)移絡(luò)合物形成機(jī)制及其若干應(yīng)用[J];大學(xué)化學(xué);1998年01期

3 周旭光,張娜,張亞秋;對(duì)苯醌與磺胺類(lèi)藥物荷移反應(yīng)的研究[J];分析化學(xué);1994年02期

4 李松蘭,廖代正,姚堅(jiān)毅,朱申杰,王士元;利用荷移帶的濃度效應(yīng)測(cè)定[5,10,15,20]-四取代基卟啉氯化鐵絡(luò)合物的含量[J];分析化學(xué);1996年09期

5 李華侃,趙鳳林,童沈陽(yáng);荷移反應(yīng)用于氨芐青霉素的測(cè)定[J];分析化學(xué);1997年01期

6 李華侃,趙桂芝,周旭光;荷移反應(yīng)用于測(cè)定煙胺羥丙茶堿[J];分析化學(xué);2000年05期

7 周萬(wàn)彬,李華侃,趙桂芝,榮玉梅;荷移分光光度法測(cè)定撲爾敏[J];分析化學(xué);2000年06期

8 汪敏,劉中英,崔辰艷;荷移反應(yīng)分光光度法測(cè)定吡呱酸[J];分析化學(xué);2001年08期

9 李華侃,趙桂芝,趙延清,李佐;克霉唑與氯冉酸的荷移反應(yīng)及其測(cè)定[J];分析化學(xué);2002年03期

10 陳彩萍,杜黎明,段亞麗,張艷;離子對(duì)締合物萃取分光光度法測(cè)定維生素K_3[J];分析化學(xué);2005年02期

本文編號(hào)：2489824