【摘要】：目的建立同時快速測定伐昔洛韋、泛昔洛韋和更昔洛韋3種化合物含量的分析方法。方法采用C18色譜柱,流動相:甲醇-0.03mol/L磷酸二氫銨溶液(用磷酸調節(jié)p H值至3.0±0.1)(V:V=35:65);流速:1.0m L/min,柱溫:35℃,檢測波長為250nm,結果更昔洛韋、伐昔洛韋和泛昔洛韋濃度分別在9.88~59.28、9.48~56.88和20.08~120.48μg/m L范圍內,與其峰面積呈良好線性關系,線性方程分別為:A=6.71×104C+7.91×104(R2=0.9997),A=4.89×104C+6.94×104(R2=0.9997)和A=1.06×104C+3.82×104(R2=0.9995);加標回收率分別為99.7%(RSD為1.0%)、100.4%(RSD為1.2%)和9.3%(RSD為1.1%)(n=9);樣品溶液在6h內穩(wěn)定。結論建立的方法簡便、準確,可同時測定核苷類抗病毒藥物的含量。
[Abstract]:Objective to establish a rapid method for simultaneous determination of valaciclovir, fanciclovir and ganciclovir. Methods A C18 column was used with a mobile phase of methanol-0.03mol/L ammonium dihydrogen phosphate solution (adjusted pH value to 3.0 鹵0.1 with phosphoric acid) (V:V=35:65). The flow rate was 1.0 mL / min, the column temperature was 35 鈩，

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