【摘要】：[目的]探討泰拉霉素在雞體內(nèi)的藥物代謝動力學過程和特征及其生物利用度。[方法]給受試健康雞分別進行靜脈注射、肌肉注射及口服泰拉霉素,給藥后在120 h內(nèi)分點心臟采血,血漿樣品經(jīng)乙腈-0.1%甲酸溶液(體積比為95∶5)沉淀,離心去蛋白,以HPLC-ESI-MS/MS法檢測雞血漿中泰拉霉素含量,最后采用藥動學軟件Winnonlin的非房室模型進行數(shù)據(jù)分析。[結果]受試雞經(jīng)靜脈注射、肌肉注射和口服給藥后藥時數(shù)據(jù)均適合非房室模型。靜脈注射給藥后,血漿清除率(CL)為17.71 m L·kg-1·h-1,穩(wěn)態(tài)表觀分布容積(Vss)為77.15 L·kg-1,消除半衰期(t1/2)為21.65 h。肌肉注射和口服泰拉霉素后,在0.25和0.5 h時,血漿濃度(Cmax)達峰值,分別為0.83和0.69 ng·m L-1,血漿中t1/2平均分別為20.96和24.33 h;生物利用度分別為92.59%和62.38%,說明肌肉注射給藥較口服吸收完全,生物利用度高。[結論]泰拉霉素肌肉注射或口服在健康雞體內(nèi)表觀分布容積大,吸收和分布迅速,達峰時間短,消除緩慢,生物利用度高。
[Abstract]:[objective] to investigate the pharmacokinetic process, characteristics and bioavailability of terramycin in chickens. [methods] healthy chickens were given intravenous injection, intramuscular injection and oral administration of terramycin respectively. Blood samples were collected from the heart within 120 hours after administration. The plasma samples were precipitated by acetonitrile-0.1% formic acid solution (volume ratio 95:5). The content of telamphenicol in chicken plasma was detected by HPLC-ESI-MS/MS, and the data were analyzed by non-atrioventricular model of the pharmacokinetic software Winnonlin. [results] the data of intravenously, intramuscular and oral administration were all suitable for non-atrioventricular model. After intravenous administration, the plasma clearance rate (CL) was 17.71 mL kg- 1 路h-1, the steady state apparent distribution volume (Vss) was 77.15 L kg-1, elimination half-life (t 1 / 2) was 21.65 h. At 0.25h and 0.5h after intramuscular injection and oral administration of terramycin, the plasma concentration of (Cmax) was 0.83 and 0.69 ng 路mL ~ (- 1), respectively, and the average level of T1 ~ (2) in plasma was 20.96 and 24.33 h, respectively. The bioavailability was 92.59% and 62.38% respectively, indicating that intramuscular administration was more complete than oral administration and the bioavailability was higher. [conclusion] the apparent distribution volume is large, the absorption and distribution is rapid, the peak time is short, the elimination is slow and the bioavailability is high in healthy chickens by intramuscular injection or oral administration of terramycin.
【作者單位】： 安徽農(nóng)業(yè)大學動物科技學院;安徽省獸藥飼料監(jiān)察所;
【基金】：安徽省教育廳重點項目(KJ2012A111)
【分類號】：R965

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相關期刊論文 前3條

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本文編號：2464046