【摘要】：海洋放線菌是研究抗結(jié)核藥物及其先導(dǎo)化合物的重要來源。以恥垢分枝桿菌(Mycobacterium smegmatis,Msm)作為指示菌,采用平板對峙法,從漳州淺灘泥土分離的77株放線菌中篩選出9株對Msm具有抑制作用的抗性放線菌,其中1株對Msm具有高效抑制作用的拮抗放線菌HY286。通過對菌株HY286的菌落形態(tài)觀察、生理生化鑒定及16S r DNA序列分析,初步鑒定其為一株馬杜拉屬放線菌。通過測定菌株HY286發(fā)酵產(chǎn)物的抗恥垢分枝桿菌MIC值、抗菌活性和細胞毒活性。結(jié)果表明,該菌株產(chǎn)生化合物的抗恥垢分枝桿菌MIC值為200μg/m L,具有較好的抗結(jié)核分枝桿菌的潛力,且對枯草芽孢桿菌、短小芽孢桿菌也具有較好的抑制作用,對He La和Hep G2的抑制率分別為89.3%和94.2%。
[Abstract]:Marine actinomycetes are important sources of antituberculous drugs and their lead compounds. Using Mycobacterium smegmatis,Msm as indicator, 9 resistant actinomycetes were screened from 77 strains of actinomycetes isolated from soil of Zhangzhou shoal by plate confrontation method. One of them is an antagonistic actinomycetes HY286., which has high inhibitory effect on Msm. The colony morphology, physiological and biochemical identification and 16s r DNA sequence analysis of the strain HY286 were preliminarily identified as an actinomycetes of Madura. The MIC value, antimicrobial activity and cytotoxic activity of the fermentation product of HY286 were determined. The results showed that the MIC value of the compound produced by the strain was 200mg / L, which had a good potential for anti-Mycobacterium tuberculosis, and also had a good inhibitory effect on Bacillus subtilis and Bacillus brevis, and it had a good inhibitory effect on Bacillus subtilis and B. brevis, and had a good inhibitory effect on Bacillus subtilis and B. brevis. The inhibition rates of He La and Hep G2 were 89. 3% and 94. 2%, respectively.
【作者單位】： 陜西省微生物研究所微生物資源中心;
【基金】：陜西省科技廳項目(2016FWPT-10) 陜西省科學(xué)院科技計劃項目(2014k-35-2)
【分類號】：R915

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