【摘要】：目的:探討精神類藥物舍曲林對人急性髓系白血病細胞株Kasumi-1的生物學效應。方法:將不同濃度舍曲林作用于Kasumi-1細胞不同時間,通過細胞計數(shù)、細胞形態(tài)學檢查、流式細胞術和Western blot分別檢測舍曲林處理前后Kasumi-1細胞增殖和凋亡情況、細胞周期分布以及相關蛋白的改變。結果:舍曲林能夠抑制Kasumi-1細胞增殖、誘導細胞凋亡;15和20μmol/L舍曲林處理Kasumi-1細胞24 h后,生長抑制率分別達到(19.00±7.37)%和(47.90±11.19)%;流式細胞術檢測結果顯示,與空白對照組相比,15和20μmol/L舍曲林處理Kasumi-1細胞24 h后,Annexin V陽性細胞百分比從(9.71±2.12)%分別上升至(20.54±2.52)%和(45.37±7.88)%(P0.01),且細胞被明顯阻滯在G0/G1期和G2/M期;此外,舍曲林還可以下調細胞內翻譯控制腫瘤蛋白(translationally controlled tumor protein,TCTP)。結論:舍曲林能夠顯著誘導Kasumi-1細胞發(fā)生凋亡,其作用機制可能與細胞內TCTP蛋白的下調相關。
[Abstract]:Objective: to investigate the biological effects of sertraline on human acute myeloid leukemia cell line Kasumi-1. Methods: Kasumi-1 cells were treated with Sertraline at different concentrations for different time. Cell count, cell morphology, flow cytometry and Western blot were used to detect the proliferation and apoptosis of Kasumi-1 cells before and after sertraline treatment. Cell cycle distribution and changes in related proteins. Results: Sertraline inhibited the proliferation of Kasumi-1 cells and induced apoptosis, and the growth inhibition rates of Kasumi-1 cells treated with sertraline for 24 h were (19.00 鹵7.37)% and (47.90 鹵11.19)%, respectively. The results of flow cytometry showed that Kasumi-1 cells were treated with 15 and 20 渭 mol/L sertraline for 24 h, compared with the control group. The percentage of Annexin V positive cells increased from (9.71 鹵2.12)% to (20.54 鹵2.52)% and (45.37 鹵7.88)%, respectively. In addition, sertraline can also down-regulate intracellular translation control tumor protein (translationally controlled tumor protein,TCTP). Conclusion: Sertraline can induce apoptosis in Kasumi-1 cells, and its mechanism may be related to the down-regulation of TCTP protein.
【作者單位】： 上海交通大學醫(yī)學院附屬瑞金醫(yī)院檢驗系上海血液學研究所醫(yī)學基因組學國家重點實驗室;
【基金】：國家自然科學基金(81170508,81470317) 上海市領軍人才"國家隊"專項資金
【分類號】：R96

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