【摘要】：目的:建立測定人血漿中培美曲塞二鈉濃度的方法。方法:血漿樣品經強酸沉淀蛋白后采用高效液相色譜法進樣測定,色譜柱為Xterra#174;RP18,流動相為乙腈-50mmol/L磷酸二氫鈉緩沖液(15∶85),流速為1.2ml/min,檢測波長為225nm。結果:培美曲塞二鈉血藥濃度在1.0~32.0mg/L范圍內線性關系良好(r=0.999 9,n=5),提取回收率為86.35%~87.25%,日內、日間RSD均≤4.21%(n=5)。最低血漿檢測質量濃度為0.05mg/L。血藥濃度較高的患者在以后易出現較高的骨髓抑制,出現不良反應的血藥濃度為(18.56±4.50)mg/L,無不良反應的血藥濃度為(14.05±6.72)mg/L,兩樣本均數經t檢驗,P0.05,差異有統計學意義。結論:該方法簡便、快速、準確、靈敏,適用于培美曲塞二鈉血藥濃度監(jiān)測及人體藥動學研究。
[Abstract]:Objective: to establish a method for the determination of pemetrexide disodium in human plasma. Methods: the plasma samples were precipitated by strong acid and the protein was determined by high performance liquid chromatography (HPLC). The chromatographic column was Xterra#174;. The mobile phase of RP18, was acetonitrile-50mmol/L sodium dihydrogen phosphate buffer (15:85), the flow rate was 1.2 ml / min, and the detection wavelength was 225 nm. Results: the plasma concentration of pemetrexide was linear in the range of 1.0~32.0mg/L (r = 0.999 9). The recovery rate was 86.35% and the RSD was less than 4.21% (n = 5) during the day. The lowest plasma mass concentration was 0.05 mg / L. Patients with higher blood drug concentration were prone to higher bone marrow suppression, and the blood drug concentration with adverse reaction was (18.56 鹵4.50) mg/L, and (14.05 鹵6.72) mg/L, without adverse drug reaction. The mean of the two samples was tested by t test and the difference was statistically significant (P 0.05). Conclusion: this method is simple, rapid, accurate and sensitive. It is suitable for monitoring the blood concentration of pemetrexide and pharmacokinetics.
【作者單位】： 徐州醫(yī)學院附屬醫(yī)院;
【基金】：徐州市社會發(fā)展科技基金(No.XZZDY1304)
【分類號】：R969.1;R96

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相關期刊論文 前4條

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