【摘要】：目的:研究藥食用真菌的釀酒酵母抑制活性及抗氧化活性。方法:將實(shí)驗(yàn)用的藥食用真菌進(jìn)行分離純化,用菌絲體進(jìn)行液體培養(yǎng),取其代謝產(chǎn)物進(jìn)行釀酒酵母抑制活性試驗(yàn),篩選具有該活性的菌株。再從活性菌株中分離出具有釀酒酵母抑制活性的化合物。采用DPPH法、Fenton法和鄰苯三酚自氧化法對7種最為常見食用真菌不同部位提取物的抗氧化活性進(jìn)行研究。結(jié)果:通過釀酒酵母抑制活性試驗(yàn),篩選出了兩種活性較好的的菌株,分別為雞樅菌和BLG-3。從雞樅菌代謝產(chǎn)物中分離出三種化合物,鑒定了其中兩種為麥角甾醇和腺嘌呤核苷。麥角甾醇在4 mg/m L濃度下平均抑菌直徑為11.7 mm,具有較好的釀酒酵母抑制活性。從BLG-3代謝產(chǎn)物中分離出一種化合物鑒定為膠霉毒素,其在4 mg/mL濃度下平均抑菌直徑為12.6 mm,具有較好的酵母抑制活性。7種食用真菌提取物對DPPH都具有一定的清除能力,其中姬菇菌柄、茶樹菇菌蓋和香菇菌蓋的提取物較其他樣品的清除效果強(qiáng);對羥基自由基除茶樹菇菌蓋提取物有一定的清除能力,其余提取物的清除能力較弱;對超氧陰離子除茶樹菇菌蓋較強(qiáng),茶樹菇菌柄和香菇菌蓋有一定的清除能力外,其余提取物的清除效果較弱。結(jié)論:由于釀酒酵母是篩選抗腫瘤藥物的良好模型,麥角甾醇和膠霉毒素對其有較好的抑制作用,因此這兩種化合物在篩選抗腫瘤藥物的過程中具有重要的研究價(jià)值;7種食用真菌不同部位提取物均具有一定的抗氧化活性,其中茶樹菇菌蓋綜合抗氧化活性最佳。
[Abstract]:Objective: to study the inhibitory and antioxidation activities of edible fungi in Saccharomyces cerevisiae. Methods: the edible fungi used in the experiment were isolated and purified. The mycelium was used for liquid culture. The metabolites of the fungi were used for the inhibition test of Saccharomyces cerevisiae and the strains with the activity were screened. The compounds with inhibitory activity of Saccharomyces cerevisiae were isolated from the active strains. The antioxidant activities of extracts from different parts of seven most common edible fungi were studied by DPPH method and pyrogallol autooxidation method. Results: through the inhibitory activity test of Saccharomyces cerevisiae, two strains with better activity were screened out, which were fir and BLG-3, respectively. Three compounds were isolated from the metabolites of abieties, and two of them were identified as ergosterol and adenosine. The average inhibitory diameter of ergosterol at 4 mg/m / L concentration was 11.7mm, which showed that ergosterol had better inhibitory activity of Saccharomyces cerevisiae. A compound isolated from the metabolites of BLG-3 was identified as collotoxin with an average inhibitory diameter of 12.6mm at the concentration of 4 mg/mL. The extracts of mushroom stalk, tea mushroom and lentinus edodes had stronger scavenging effect than other samples, and had a certain scavenging ability to hydroxyl free radical, but the other extracts had weak scavenging ability. For superoxide anion, the scavenging effect of the other extracts was weak except for the strong tea mushroom cap, the tea mushroom stalk and the lentinus edodes cover. Conclusion: as Saccharomyces cerevisiae is a good model for screening antitumor drugs, ergosterol and collotoxin have a good inhibitory effect on it. Therefore, these two compounds have important research value in the process of screening anti-tumor drugs. The extracts of 7 edible fungi have certain antioxidant activities, among which the comprehensive antioxidant activity of tea mushroom mulches is the best.
【學(xué)位授予單位】：貴州醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R915

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