本文選題：海洋真菌 + Aspergillus��； 參考：《天然產(chǎn)物研究與開發(fā)》2017年07期

【摘要】：通過多種色譜柱層析(包括凝膠Sephadex LH-20、正相硅膠、中壓液相色譜和高效液相色譜)分離手段,從來源于南海柳珊瑚Melitodessquamata的一株共附生真菌Aspergillus sp.SCSIO 41501的發(fā)酵液中分離純化了11個含氮化合物,它們的化學(xué)結(jié)構(gòu)通過核磁共振和質(zhì)譜等技術(shù)分別鑒定為13-(S)-赭曲霉素A(1)、13-(S)-甲酯化赭曲霉素A(2)、13-(R)-赭曲霉素A(3)、4-(R)-OH-赭曲霉素A(4)、18-OH-赭曲霉素A(5)、transtorine(6)、quinolactacin C1(7)、marinamide(8)、methyl marinamide(9)、aspergilliamide(10)、anoectochine(11)。所有化合物進行了抗菌實驗,其中化合物2對大腸桿菌、枯草芽孢桿菌、金黃色葡萄球菌具有較強的活性,其MIC值分別為31.25、7.8、15.6μg/m L,化合物3顯示中等抗菌活性,其MIC值分別為62.5、31.25、62.5μg/m L。
[Abstract]:It was separated by a variety of chromatographic column chromatography (including gel Sephadex LH-20, silica gel, medium pressure liquid chromatography and high performance liquid chromatography). Eleven nitrogen-containing compounds were isolated and purified from the fermentation broth of a co-epiphytic fungus Aspergillus sp. SCSIO 41501 from Melitodessquamata in the South China Sea. Their chemical structures were identified by NMR and MS techniques as 13 Szapin, ochratoxin A, 13 -S ~ (1) -Methylated Aspergillus aspergilli (AJ), 13 ~ (13) -Rn-OH-1, 4 ~ (4) R ~ (-) -OH -ochratin A _ (418-OH) -OHochisin A _ (418-OH) -OHZ, A _ (418-OH) -Ochratin A _ (5) Toxolactacin in C _ (1) ~ (7) a marinamelintosine _ (8) marmarinamide9e (a) aspergillium spp. (10) a kind of anectochytochinec (1), a kind of anectochinetochlor, a kind of aspergillea spp. All the compounds were tested for antimicrobial activity. Compound 2 had strong activity against Escherichia coli, Bacillus subtilis and Staphylococcus aureus with MIC values of 31.257.8U 15.6 渭 g / mL and 62.5 渭 g / mL for compound 3. The MIC values of compound 3 were 62.5 渭 g / mL and 31.25 渭 g / mL, respectively.
【作者單位】： 中國科學(xué)院南海海洋研究所中國科學(xué)院海洋生物資源可持續(xù)利用重點實驗室;中國科學(xué)院大學(xué);
【基金】：國家自然科學(xué)基金(41376160;81673326)
【分類號】：R915
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本文編號：2032156