本文選題：馬兜鈴酸 + 中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白��； 參考：《中國(guó)新藥與臨床雜志》2015年07期

【摘要】：目的探討腎損傷標(biāo)志物中性粒細(xì)胞明膠酶相關(guān)脂質(zhì)運(yùn)載蛋白(NGAL)、腎損傷因子1(KIM-1)和白細(xì)胞介素18(IL-18)在馬兜鈴酸誘導(dǎo)的大鼠腎損傷中的變化特征及其組合檢測(cè)的意義。方法取Wistar大鼠隨機(jī)分為馬兜鈴酸(25 mg·kg-1)組和對(duì)照組(等容量生理鹽水)各12只,隔兩日一次尾靜脈注射給藥,共兩周。于不同時(shí)間點(diǎn)采集血、尿樣,于末次給藥和停藥一周后分批解剖處理。生化分析儀檢測(cè)血清尿素氮(BUN)、肌酐(Cr)含量,酶聯(lián)免疫吸附法(ELISA)檢測(cè)尿液NGAL、KIM-1、IL-18濃度。光鏡下觀察腎臟病理組織形態(tài)學(xué)改變。采用ROC曲線比較各血清或尿液標(biāo)志物及其組合的曲線面積。結(jié)果馬兜鈴酸組大鼠腎小管病理?yè)p傷明顯,停藥后亦未恢復(fù);與對(duì)照組相比,血清BUN、Cr于末次給藥后顯著升高(P0.05),停藥1周后均恢復(fù),尿液NGAL、KIM-1和IL-18于不同時(shí)間點(diǎn)明顯升高(P0.01)。ROC分析顯示其組合檢測(cè)可提高效能,尤其NGAL+KIM-1和NGAL+KIM-1+IL-18組合的曲線下面積分別為0.928和0.927,遠(yuǎn)高于BUN、Cr及其組合BUN+Cr。結(jié)論尿液NGAL、KIM-1和IL-18的組合檢測(cè)更能提高檢驗(yàn)效能,可預(yù)測(cè)或提示馬兜鈴酸誘導(dǎo)的腎小管損傷。
[Abstract]:Objective to investigate the changes of neutrophil gelatinase-associated lipid carrier protein (NGALL), renal injury factor (KIM-1) and interleukin-18 (IL-18) in rat kidney injury induced by aristolochic acid. Methods Wistar rats were randomly divided into two groups: control group (n = 12) and control group (n = 12). Blood and urine samples were collected at different time points. Serum bun (bun) and creatinine (Cr) were determined by biochemical analyzer, and urine NGALL KIM-1 (IL-18) was detected by enzyme linked immunosorbent assay (Elisa). The histopathological changes of kidney were observed under light microscope. The ROC curve was used to compare the area of each serum or urine marker and its combination curve. Results the pathological injury of renal tubules in aristolochic acid group was obvious and did not recover after drug withdrawal, compared with the control group, the serum bun Cr increased significantly after the last administration of aristolochic acid, and all recovered after 1 week of withdrawal. Urine NGALL KIM-1 and IL-18 increased significantly at different time points P0.01U 路ROC analysis showed that the combined detection could improve the efficiency, especially the area under the curve of the combination of NGAL KIM-1 and NGAL KIM-1 IL-18 was 0.928 and 0.927 respectively, which was much higher than that of Buchon Cr and its combination BUN Cr. Conclusion the combined detection of urinary NGALL KIM-1 and IL-18 can improve the efficiency and predict the renal tubule injury induced by aristolochic acid.
【作者單位】： 上海中醫(yī)藥大學(xué)藥物安全評(píng)價(jià)研究中心;
【基金】：國(guó)家“重大新藥創(chuàng)制”科技重大專項(xiàng)項(xiàng)目(2009ZX09502-002) 國(guó)家重點(diǎn)基礎(chǔ)研究發(fā)展計(jì)劃(973計(jì)劃)(2009CB522807) 上海市教委預(yù)算內(nèi)項(xiàng)目(2010JW30)
【分類號(hào)】：R965.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 孫華寶;葉德強(qiáng);曹立;梁青;呂定英;陳國(guó)棟;;logistic回歸和ROC曲線評(píng)價(jià)8種原發(fā)性肝癌實(shí)驗(yàn)室診斷指標(biāo)的臨床價(jià)值[J];廣東醫(yī)學(xué);2010年20期

2 劉寶利;楊寶友;鄭桂敏;孫明霞;趙文景;張勝容;;Logistic回歸和ROC曲線綜合評(píng)價(jià)檢測(cè)四種尿蛋白排泄對(duì)早期腎小球疾病的診斷價(jià)值[J];中國(guó)中西醫(yī)結(jié)合腎病雜志;2011年08期

3 孫驊;劉志紅;王生余;秦衛(wèi)松;陳朝紅;黎磊石;;馬兜鈴酸誘導(dǎo)腎小管上皮細(xì)胞損傷中Ngal和Kim-1的變化及其意義[J];腎臟病與透析腎移植雜志;2007年04期

4 湯天鳳;曾彩虹;;腎臟疾病的篩查:傳統(tǒng)指標(biāo)與新的生物學(xué)標(biāo)記物[J];腎臟病與透析腎移植雜志;2009年02期

5 趙艷玲,王宗謙,馮江敏,王力寧,劉丹燕,張永紅;前列地爾防治大鼠馬兜鈴酸腎病纖維化[J];中國(guó)新藥與臨床雜志;2005年10期

6 劉潤(rùn)幸;使用SPSS作多變量觀察值的ROC曲線分析[J];中國(guó)公共衛(wèi)生;2003年09期

7 范海燕;孟凡云;章陽(yáng);魏仲香;關(guān)眾;;Logistic回歸及ROC曲線綜合評(píng)價(jià)CA15-3、CA125、CK19、CEA、AFP和CA199在乳腺癌術(shù)后監(jiān)測(cè)中的應(yīng)用價(jià)值[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2010年11期

8 李一飛;徐婷婷;姚廣濤;金若敏;;尿液NGAL,KIM-1,IL-18在商陸所致的大鼠腎損傷中的變化特征及其聯(lián)合檢測(cè)的意義[J];中國(guó)中藥雜志;2012年23期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 趙艷玲;王宗謙;馮江敏;周希靜;谷定英;徐玉蘭;;強(qiáng)的松對(duì)大鼠馬兜鈴酸腎病腎組織纖維化的影響[J];安徽醫(yī)學(xué);2008年04期

2 付超;王海;齊軍;王小兵;田海梅;李艷芬;李茉;吳凡;常青云;劉珊;張偉;;胰腺癌患者血清巨噬細(xì)胞抑制因子1的臨床價(jià)值研究[J];癌癥進(jìn)展;2011年02期

3 雷建華;楊旭;賀興鄂;黃力;梁駿;田沂;;HBsAg濃度與HBV復(fù)制水平的相關(guān)性分析[J];中國(guó)輸血雜志;2006年05期

4 郭建;高福云;周舒;;馬兜鈴酸腎病的研究進(jìn)展[J];中華中醫(yī)藥雜志;2009年09期

5 張振東;孫麗君;羅瑩;李欣;孫冬梅;;應(yīng)用ROC曲線評(píng)價(jià)血清β-hCG、黃體酮和子宮內(nèi)膜厚度對(duì)異位妊娠的診斷價(jià)值[J];重慶醫(yī)學(xué);2011年26期

6 許勇芝;陳麗萍;劉華鋒;陶靜莉;唐德q，

本文編號(hào)：1959193