本文選題：微機(jī)電系統(tǒng) + 植入式微電極陣列��； 參考：《納米技術(shù)與精密工程》2017年06期

【摘要】：左旋多巴(L-DOPA)是帕金森癥的主要治療藥物之一,長(zhǎng)期使用具有神經(jīng)毒性,其作用機(jī)理復(fù)雜.為了研究L-DOPA對(duì)大腦紋狀體谷氨酸神經(jīng)通路在體神經(jīng)元的影響,采用微機(jī)電系統(tǒng)(MEMS)技術(shù)制備了一種16通道植入式微電極陣列(MEA)芯片,其上集成了直徑10μm的電生理、電化學(xué)雙模信號(hào)檢測(cè)位點(diǎn),能夠?qū)崟r(shí)記錄腦內(nèi)神經(jīng)遞質(zhì)谷氨酸和動(dòng)作電位(spike)、局部場(chǎng)電位(LFP)等神經(jīng)電信號(hào)的同步變化.將這種MEA植入大鼠紋狀體,在皮層施加L-DOPA(100μL,2 mmol/L)刺激后,隨著紋狀體內(nèi)谷氨酸濃度的迅速增加,神經(jīng)元spike發(fā)放頻率和LFP低頻段功率同步衰減.實(shí)驗(yàn)結(jié)果從神經(jīng)元層次在體驗(yàn)證了L-DOPA的神經(jīng)毒性,可為帕金森癥治療藥理研究提供線索.
[Abstract]:L-DOPA is one of the main drugs for Parkinson's disease. It has neurotoxicity and complex mechanism. In order to study the effect of L-DOPA on the in vivo neurons of the glutamate pathway in the striatum of the brain, a 16 channel implanted microelectrode array (MEA) chip was fabricated by using the microelectromechanical system (MEMS) technique. The microelectrode array was integrated with electrophysiology of 10 渭 m in diameter. The detection site of electrochemical double mode signal can record the synchronous changes of neurotransmitter glutamic acid and action potential spike, local field potential (LFPP) and so on in the brain in real time. The MEA was implanted into the striatum of rats and stimulated by L-DOPA(100 渭 L ~ (2 mmol / L) in cortex. With the rapid increase of glutamate concentration in the striatum, the frequency of neuronal spike emission and the power of LFP decreased synchronously. The results confirm the neurotoxicity of L-DOPA in vivo and provide clues for the pharmacological study of Parkinson's disease.
【作者單位】： 中國(guó)科學(xué)院電子學(xué)研究所傳感技術(shù)聯(lián)合國(guó)家重點(diǎn)實(shí)驗(yàn)室;中國(guó)科學(xué)院大學(xué);
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(61527815,31500800,61501426,61471342) 國(guó)家重點(diǎn)研發(fā)計(jì)劃資助項(xiàng)目(2017YFA0205900) 北京市科技計(jì)劃資助項(xiàng)目(Z141100000214002,Z141102003414014) 中國(guó)科學(xué)院重點(diǎn)資助項(xiàng)目(KJZD-EW-L11-2)
【分類號(hào)】：R96
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本文編號(hào)：1958728