本文選題：甲醛　切入點：慢性吸入暴露　出處：《環(huán)境與健康雜志》2017年06期

【摘要】：目的研究甲醛慢性吸入染毒致雄性小鼠生殖細胞遺傳毒性。方法 SPF級雄性ICR小鼠40只,隨機分為4組:低劑量組、高劑量組、陰性對照組和陽性對照組,每組10只,低、高劑量組染毒劑量分別為1、10 mg/m~3,用靜式吸入的方法進行染毒,每天一次,每次2 h,連續(xù)染毒20周,陽性對照組給予環(huán)磷酰胺。小鼠染毒后繼續(xù)飼養(yǎng)5周。觀察并計算精子畸形率。結果陰性對照組、低劑量組和高劑量組的睪丸臟器系數(shù)分別為(0.81±0.13)%、(0.82±0.12)%和(0.78±0.12)%,各組之間差異無統(tǒng)計學意義(P0.05);陰性對照組、陽性對照組、低劑量組和高劑量組的精子畸形率分別為4.1%、6.4%、11.5%和11.3%,與陰性對照組比較,各染毒組精子畸形率明顯增加,差異有統(tǒng)計學意義(P0.01);且染毒劑量與精子畸形率之間存在劑量-反應關系(r_s=0.888,P0.01)。結論甲醛慢性吸入染毒可引起小鼠精子畸形率升高,且染毒劑量與精子畸形率之間存在劑量-反應關系。
[Abstract]:Objective to study the genetic toxicity of male mice germ cells induced by chronic inhalation of formaldehyde. Methods Forty SPF grade male ICR mice were randomly divided into four groups: low dose group, high dose group, negative control group and positive control group. In the high dose group, the doses were 1 ~ 10 mg / m ~ (-3), respectively. The dose was inhaled once a day for 2 hours for 20 weeks. The positive control group was given cyclophosphamide. The mice were fed with cyclophosphamide for 5 weeks. The rate of sperm abnormality was observed and calculated. The testicular organ coefficients of low dose group and high dose group were 0.82 鹵0.12% and 0.78 鹵0.12%, respectively. There was no significant difference between the two groups, and the sperm deformity rates of negative control group, positive control group, low dose group and high dose group were 4.16.44.45% and 11.33.Compared with the negative control group, respectively, the testicular visceral organ coefficients of the low dose group and the high dose group were 0.82 鹵0.12% and 0.78 鹵0.12%, respectively. There was a dose-response relationship between the dose of exposure and the rate of sperm malformation. Conclusion chronic inhalation of formaldehyde can increase the rate of sperm malformation in mice, and there is a dose-response relationship between the dose-response and the rate of sperm malformation. Conclusion the abnormal rate of spermatozoa in mice can be increased by chronic inhalation of formaldehyde, and there is a dose-response relationship between the dose of exposure and the rate of sperm malformation. There was a dose-response relationship between the dose and the rate of sperm malformation.
【作者單位】： 海南醫(yī)學院公共衛(wèi)生學院;
【基金】：國家自然科學基金(81160351) 海南省自然科學基金(811202) 海南省大學生創(chuàng)新創(chuàng)業(yè)訓練計劃項目(20160106) 海南醫(yī)學院創(chuàng)新創(chuàng)業(yè)訓練計劃項目(HYCX2015031)
【分類號】：R994.6

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