本文選題：斑馬魚　切入點(diǎn)：吉非替尼　出處：《重慶醫(yī)學(xué)》2017年32期 　論文類型：期刊論文

【摘要】：目的研究吉非替尼對(duì)斑馬魚早期胚胎發(fā)育及abcb4腫瘤耐藥基因表達(dá)的影響。方法實(shí)驗(yàn)動(dòng)物采用斑馬魚,實(shí)驗(yàn)分為吉非替尼組、阿霉素和吉非替尼的混合液組、空白對(duì)照組。將吉非替尼稀釋成不同濃度梯度的藥液,分別處理受精后0.5~1.5h(0.5~1.5hpf)的斑馬魚胚胎,觀察胚胎和幼魚的死亡率、孵化率及畸形率變化。統(tǒng)計(jì)各濃度不同時(shí)間點(diǎn)的胚胎死亡率,計(jì)算出IC_(50)的數(shù)值;統(tǒng)計(jì)48hpf和72hpf的胚胎孵化率及120hpf的畸形率;收集不同組別的斑馬魚胚胎,通過實(shí)時(shí)熒光定量PCR,檢測(cè)斑馬魚胚胎中abcb4基因的表達(dá)量。結(jié)果計(jì)算出吉非替尼在斑馬魚胚胎中的IC_(50)為16.18μmol/L。與對(duì)照組比較,高劑量吉非替尼(≥20μmol/L)顯著抑制胚胎的孵化,并有明顯的胚胎致死效應(yīng)和致畸效應(yīng);吉非替尼組的斑馬魚胚胎abcb4基因的mRNA水平變化不顯著,而阿霉素和吉非替尼的混合液組的abcb4基因的mRNA水平有明顯變化(P0.05)。結(jié)論吉非替尼對(duì)斑馬魚早期胚胎中abcb4腫瘤耐藥基因的表達(dá)水平無明顯影響(P0.05),但是吉非替尼能逆轉(zhuǎn)易耐藥藥物阿霉素的耐藥作用,提示使用斑馬魚可以構(gòu)建腫瘤耐藥模型。
[Abstract]:Objective to study the effects of gefitinib on the development of early embryos and the expression of drug-resistant genes in abcb4 tumor of zebrafish. Methods zebrafish were divided into three groups: gifitinib group, doxorubicin and gefitinib mixture group. In the blank control group, the zebrafish embryos were treated with different concentration gradient solution of gefitinib, and the death rates of embryos and young fish were observed, and the embryos of zebrafish were treated with 0.5 ~ (1.5) h ~ (-1) and 0.5 ~ (5) h ~ (PF) after fertilization, respectively. Change of hatching rate and deformity rate. Count the embryo mortality rate at different time points of different concentration, calculate the value of ICSP 50), calculate the embryo hatchability rate of 48hpf and 72hpf and the deformity rate of 120hpf; collect different groups of zebrafish embryos, The expression of abcb4 gene in zebrafish embryos was detected by real-time fluorescence quantitative PCR. The ICSP 50 of gifitinib in zebrafish embryos was calculated to be 16.18 渭 mol / L. Compared with the control group, high dose of gifitinib (鈮，

本文編號(hào)：1633958