發(fā)布時(shí)間：2018-03-16 17:33

  本文選題：異硫氰酸熒光素標(biāo)記的枸杞多糖　切入點(diǎn)：藥代動(dòng)力學(xué)　出處：《食品科學(xué)》2015年02期 　論文類型：期刊論文

【摘要】：建立大鼠血漿中異硫氰酸熒光素標(biāo)記的枸杞多糖(fluorescein isothiocyanate labeled Lycium barbarumpolysaccharides,LBP-FITC)的熒光定量分析方法,線性方程為y=1 378.7x-14.98(R2=0.998 9),血漿中LBP-FITC質(zhì)量濃度在0.05~10μg/m L范圍內(nèi)與熒光強(qiáng)度呈良好的線性關(guān)系。方法的精密度、回收率以及穩(wěn)定性考察分析證明該方法符合生物樣品體內(nèi)定量分析的要求。單次灌胃給予大鼠LBP-FITC后,以藥代動(dòng)力學(xué)Win Nonlin軟件采用非房室模型擬合大鼠單次口服LBP-FITC的經(jīng)時(shí)血藥質(zhì)量濃度數(shù)據(jù),計(jì)算主要的藥代動(dòng)力學(xué)參數(shù)為最大峰質(zhì)量濃度(Cmax)(2.39±0.71)μg/m L、達(dá)峰時(shí)間(tmax)(1.17±0.41)h;藥時(shí)曲線下面積AUC0-t為(45.85±7.12)(μg?h)/m L,AUC0-∞為(127.31±39.00)(μg?h)/m L;半衰期(t1/2)為(80.32±32.70)h;清除率為(1 693.96±492.47)m L/kg;平均滯留時(shí)間為(20.64±1.36)h。
[Abstract]:A method for quantitative analysis of fluorescein isothiocyanate labeled Lycium barumPolysaccharide LBP-FITCin rat plasma with fluorescein isothiocyanate was established. The linear equation is YY 1 378.7x-14.98 9 渭 g / mL LBP-FITC concentration in plasma is linear with fluorescence intensity in the range of 0.05 ~ 10 渭 g / m ~ (-1). The precision of the method is obtained. The results of recovery and stability analysis showed that the method met the requirements of quantitative analysis of biological samples in vivo. After single oral administration of LBP-FITC in rats, The pharmacokinetic Win Nonlin software was used to fit the plasma drug concentration data of single oral LBP-FITC in rats with a non-atrioventricular model. The main pharmacokinetic parameters were calculated as the maximum peak concentration of C _ (max) 2.39 鹵0.71) 渭 g / m ~ (L), the peak time was 1.17 鹵0.41 h, and the area under the drug time curve was AUC0-t = 45.85 鹵7.12 (渭 g 路L ~ (-1)). The value of AUC0- 鈭，

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