本文選題：人細(xì)小病毒B　切入點(diǎn)：血漿　出處：《軍事醫(yī)學(xué)》2015年03期 　論文類(lèi)型：期刊論文

【摘要】：目的檢測(cè)混合原料血漿和凝血因子類(lèi)產(chǎn)品中人細(xì)小病毒B19(human parvovirus B19,B19病毒)核酸,分析我國(guó)原料血漿及凝血因子類(lèi)產(chǎn)品中B19病毒的污染情況。方法針對(duì)B19病毒的保守區(qū)域NS1區(qū)合成引物及探針,采用Taq Man實(shí)時(shí)定量PCR法檢測(cè)混合原料血漿和4類(lèi)凝血因子類(lèi)產(chǎn)品中B19病毒核酸。結(jié)果混合原料血漿的B19病毒核酸的陽(yáng)性率為59.49%(116/195),最高濃度可達(dá)1.35×1010拷貝/ml。因子Ⅷ、凝血酶、纖維蛋白原以及凝血酶原復(fù)合物的陽(yáng)性率分別為93.55%(29/31)、100%(10/10)、85.71%(6/7)和88.89%(8/9)。結(jié)論我國(guó)混合原料血漿與凝血因子類(lèi)產(chǎn)品中B19病毒的污染率較高,有必要進(jìn)行原料血漿的B19病毒篩查,對(duì)于保障血液制品的病毒安全性具有重要意義。
[Abstract]:Objective to detect the nucleic acid of human parvovirus B19 parvovirus B19 virus in mixed raw plasma and coagulation factor products. The contamination of B19 virus in raw plasma and coagulation factor products in China was analyzed. Methods primers and probes were synthesized for the conserved NS1 region of B19 virus. Taq Man real-time quantitative PCR method was used to detect B19 virus nucleic acid in mixed raw plasma and four kinds of coagulation factor products. Results the positive rate of B19 virus nucleic acid in plasma of mixed raw material was 59.490.116 / 195. The highest concentration of B19 virus nucleic acid was 1.35 脳 10 10 copies / ml 路ml.Factor 鈪，

本文編號(hào)：1577685