本文關(guān)鍵詞： 分子熒光光譜法 分子對接 分子動(dòng)力學(xué)模擬 ′-羥基- ′ -四溴二苯醚 人血清白蛋白　出處：《理化檢驗(yàn)(化學(xué)分冊)》2017年01期 　論文類型：期刊論文

【摘要】：結(jié)合分子熒光光譜法、分子對接和分子動(dòng)力學(xué)模擬等方法研究了在生理?xiàng)l件(pH 7.40)下2′-羥基-2,3′,4,5-四溴二苯醚(2′-OH-BDE-68)與人血清白蛋白(HSA)的相互作用及其對HSA構(gòu)象的影響。結(jié)果表明:1 2′-OH-BDE-68對HSA內(nèi)源性熒光的猝滅屬靜態(tài)猝滅和非輻射能量轉(zhuǎn)移過程;2兩者之間的反應(yīng)是以疏水作用力為主輔以微弱的靜電作用的自發(fā)反應(yīng)過程;3 2′-OH-BDE-68與HSA的結(jié)合在10ns內(nèi)趨于平衡,結(jié)合位點(diǎn)在SiteⅠ;4復(fù)合物的形成使HSA分子中的色氨酸的疏水性增加,從而HSA的二級(jí)結(jié)構(gòu)發(fā)生改變。
[Abstract]:Combined with molecular fluorescence spectroscopy, Molecular docking and molecular dynamics simulation methods were used to study the interaction between 2OH-BDE-68) and human serum albumin (HSA) under physiological conditions (pH 7.40). The results showed that the conformation of HSA was affected by the conformation of human serum albumin (HSA). The fluorescence quenching is a static quenching process and a non-radiative energy transfer process. The spontaneous process of fluorescence quenching is the spontaneous reaction process in which hydrophobic force is dominant and the electrostatic action is weak. The combination of OH-BDE-68 and HSA tends to balance in 10 ns. The formation of binding site in Site 鈪，

本文編號(hào)：1515035