本文關鍵詞： 分子熒光光譜法 分子對接 分子動力學模擬 ′-羥基- ′ -四溴二苯醚 人血清白蛋白　出處：《理化檢驗(化學分冊)》2017年01期 　論文類型：期刊論文

【摘要】：結合分子熒光光譜法、分子對接和分子動力學模擬等方法研究了在生理條件(pH 7.40)下2′-羥基-2,3′,4,5-四溴二苯醚(2′-OH-BDE-68)與人血清白蛋白(HSA)的相互作用及其對HSA構象的影響。結果表明:1 2′-OH-BDE-68對HSA內源性熒光的猝滅屬靜態(tài)猝滅和非輻射能量轉移過程;2兩者之間的反應是以疏水作用力為主輔以微弱的靜電作用的自發(fā)反應過程;3 2′-OH-BDE-68與HSA的結合在10ns內趨于平衡,結合位點在SiteⅠ;4復合物的形成使HSA分子中的色氨酸的疏水性增加,從而HSA的二級結構發(fā)生改變。
[Abstract]:Combined with molecular fluorescence spectroscopy, Molecular docking and molecular dynamics simulation methods were used to study the interaction between 2OH-BDE-68) and human serum albumin (HSA) under physiological conditions (pH 7.40). The results showed that the conformation of HSA was affected by the conformation of human serum albumin (HSA). The fluorescence quenching is a static quenching process and a non-radiative energy transfer process. The spontaneous process of fluorescence quenching is the spontaneous reaction process in which hydrophobic force is dominant and the electrostatic action is weak. The combination of OH-BDE-68 and HSA tends to balance in 10 ns. The formation of binding site in Site 鈪，

本文編號：1515035