本文關(guān)鍵詞： 基質(zhì)硬度 二維/三維細(xì)胞培養(yǎng) 細(xì)胞形態(tài) 細(xì)胞攝取 聚苯乙烯納米球　出處：《藥學(xué)學(xué)報》2017年08期 　論文類型：期刊論文

【摘要】：以聚苯乙烯納米球為研究對象,研究基質(zhì)硬度對二維(2D)和三維(3D)培養(yǎng)的乳腺癌細(xì)胞攝取行為的影響。通過光催化聚合反應(yīng)法制備硬度可調(diào)的聚丙烯酰胺凝膠,并在凝膠表面共價修飾膠原以支持細(xì)胞黏附,構(gòu)建2D細(xì)胞培養(yǎng)模型;選擇Ⅰ型鼠尾膠原構(gòu)建3D細(xì)胞培養(yǎng)模型;分別接種人乳腺癌細(xì)胞MCF-7于聚丙烯酰胺凝膠表面或混懸于膠原凝膠中進(jìn)行3D細(xì)胞培養(yǎng);20 nm和50 nm聚苯乙烯納米球懸液與2D或3D培養(yǎng)的細(xì)胞共孵育,采用激光共聚焦顯微鏡對細(xì)胞形態(tài)和納米球攝取效率進(jìn)行定性和定量考察。本研究發(fā)現(xiàn)在2D和3D培養(yǎng)條件下,基質(zhì)硬度均參與調(diào)控腫瘤細(xì)胞肌動蛋白骨架的重構(gòu)并間接影響不同粒徑大小的納米制劑的攝取行為。
[Abstract]:The polystyrene nanospheres were used as the research object. To study the effect of substrate hardness on the uptake behavior of breast cancer cells cultured with 2D and 3D), a polyacrylamide gel with adjustable hardness was prepared by photocatalytic polymerization. The collagen was covalently modified on the gel surface to adhere to the Sertoli cells, and the 2D cell culture model was constructed. The model of 3D cell culture was established by selecting collagen of type 鈪，

本文編號：1450547