發(fā)布時間：2018-01-21 05:04

  本文關(guān)鍵詞： 上轉(zhuǎn)換熒光 碳點 共振光散射技術(shù) 鹽酸巴馬汀 水熱法　出處：《西南大學》2014年碩士論文　論文類型：學位論文

【摘要】：碳點作為一種新型的熒光碳納米材料,擁有特殊的熒光性能,如激發(fā)和發(fā)射波長可調(diào)、光穩(wěn)定性好、無光閃爍現(xiàn)象等,其獨特的發(fā)光性質(zhì)和生物相容性在生化分析、細胞成像及藥物檢測等領(lǐng)域具有較好的應用潛力。共振光散射分析方法因其操作簡單、靈敏度高等優(yōu)點而廣泛應用于生命科學、藥物分析等領(lǐng)域。本文從建立天然小分子藥物的分析方法出發(fā),建立了一種高靈敏檢測鹽酸巴馬汀的方法,此外探索出了一種新的低溫一步法制備高熒光量子產(chǎn)率碳點的方法,并將其應用在天然小分子藥物檢測中。具體內(nèi)容包括以下幾方面： (1)鹽酸巴馬汀與桑色素相互作用的共振光散射譜研究。在pH為4.6的HAc-NaAc緩沖液中,桑色素與鹽酸巴馬汀(PaＨＨ)發(fā)生化學反應,由于靜電引力形成離子締合物,導致體系在308nm處的共振光散射信號顯著增強。增強的共振光散射信號與PaＨＨ的濃度在0.08-1.0μmol/L之間呈良好的線性關(guān)系,由此建立了一種測定PaＨＨ的共振光散射分析方法,檢測限為8.0nmol/L。研究了反應機理,確定了最佳的反應條件,并成功用于黃藤素片及黃藤素膠囊中鹽酸巴馬汀含量的測定,RSD≤3.3%。 (2)一步低溫水熱法合成高量子產(chǎn)率熒光碳點。以檸檬酸為碳源,聚乙烯亞胺為鈍化劑一步水熱合成熒光碳點。該合成方法簡便、經(jīng)濟、對環(huán)境友好。制備的碳點的熒光量子產(chǎn)率高達53%,且具有上轉(zhuǎn)換熒光性質(zhì)。研究了碳點的粒徑、發(fā)光性質(zhì)和抗干擾能力等。實驗發(fā)現(xiàn)該碳點粒徑為4nm左右,具有良好的水溶性、光穩(wěn)定性和生物相容性。研究發(fā)現(xiàn)該碳點對pH較敏感,可用于pH傳感。 (3)研究了碳點與桑色素相互作用的熒光光譜。在pH4.0HAc-NaAc環(huán)境中,表面帶有大量氨基的碳點與帶負電荷的桑色素由于靜電引力作用,使碳點在449nm處的熒光信號和位于451nm處的上轉(zhuǎn)換熒光信號發(fā)生猝滅,降低的熒光信號強度和上轉(zhuǎn)換熒光信號強度與桑色素的濃度在0～300μmol/L之間呈良好的線性關(guān)系,檢測限為0.6μmol/L。據(jù)此建立了雙熒光定量檢測桑色素的方法。討論了體系的反應條件和外來物質(zhì)的干擾,建立的方法成功用于尿樣中痕量桑色素的測定,RSD≤4.5%。
[Abstract]:As a new kind of fluorescent carbon nanomaterials, carbon dots have special fluorescent properties, such as adjustable excitation and emission wavelength, good optical stability, no light flicker and so on. Its unique luminescent properties and biocompatibility have good application potential in the fields of biochemical analysis, cell imaging and drug detection. Resonance light scattering (RLS) analysis method is easy to operate. Because of its high sensitivity, it has been widely used in life sciences, drug analysis and other fields. In this paper, a highly sensitive method for the detection of palmatine hydrochloride was established based on the establishment of a method for the analysis of natural small molecular drugs. In addition, a new low-temperature one-step method for the preparation of high fluorescence quantum yield carbon points was developed and applied to the detection of natural small molecular drugs. 1) Resonance Light scattering Spectroscopy (RLS) study of the interaction between palmatine hydrochloride and mulberry pigment. In the HAc-NaAc buffer with pH 4. 6, mulberry pigment reacts with PahH hydrochloride. An ion association complex is formed by electrostatic gravity. As a result, the resonance light scattering signal of the system at 308 nm is significantly enhanced, and the enhanced resonance light scattering signal has a good linear relationship with the concentration of PaHH in the range of 0.08-1.0 渭 mol/L. A method for the determination of PaHH by resonance light scattering (RLS) was established. The detection limit was 8.0 nmol / L. The reaction mechanism was studied and the optimum reaction conditions were determined. The method has been successfully applied to the determination of palmatine hydrochloride in Huangtenin tablets and Huangtenin capsules with RSD 鈮，

本文編號：1450553