本文關(guān)鍵詞：高效液相色譜法測(cè)定大鼠血漿和肺泡灌洗液中環(huán)丙沙星濃度　出處：《中國臨床藥理學(xué)雜志》2017年09期 　論文類型：期刊論文

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【摘要】：目的建立一種測(cè)定大鼠血漿和肺泡灌洗液中環(huán)丙沙星濃度的高效液相色譜(HPLC)法。方法用甲醇直接蛋白沉淀法、去離子水直接稀釋法分別對(duì)血漿和肺泡灌洗液進(jìn)行處理,離心取上清,以莫西沙星為內(nèi)標(biāo),用HPLC測(cè)定。色譜柱:Agilent SB-C18柱(4.6 mm×150 mm,5μm),流動(dòng)相:乙腈-0.1%甲酸水,流速:1.0 m L·min~(-1),柱溫:25℃,進(jìn)樣量:15μL�？疾煸摲椒ǖ膶傩浴�(biāo)準(zhǔn)曲線與最低定量下限、精密度與回收率、穩(wěn)定性。結(jié)果血漿和肺泡灌洗液中的內(nèi)源性物質(zhì)不影響環(huán)丙沙星的測(cè)定。在血漿和肺泡灌洗液中,環(huán)丙沙星分別在1.00~100.00μg·m L~(-1)(r=0.999 6)、0.05~2.00μg·m L~(-1)(r=0.998 5)內(nèi)線性關(guān)系良好,最低定量下限分別為1.00和0.05μg·m L~(-1)。環(huán)丙沙星在血漿和肺泡灌洗液中的批內(nèi)精密度分別為0.49%~3.77%和2.21%~5.00%,批間精密度分別為7.72%~8.83%和6.84%~9.51%,提取回收率分別為104.61%~113.29%和100.65%~103.96%;環(huán)丙沙星血漿和肺泡灌洗液穩(wěn)定性符合相關(guān)要求。結(jié)論該方法簡單、準(zhǔn)確、可操作性強(qiáng),適用于伏立康唑大鼠血漿及肺泡灌洗液濃度測(cè)定。
[Abstract]:Objective to establish a high performance liquid chromatography (HPLC) method for the determination of ciprofloxacin in rat plasma and alveolar lavage fluid. Plasma and alveolar lavage fluid were treated by direct dilution of deionized water. The supernatant was centrifuged and moxifloxacin was used as the internal standard. HPLC was used to determine the content of acetonitrile-0.1% formic acid in the mobile phase. The chromatographic column was 4.6 mm 脳 150 mm ~ 5 渭 m 路m ~ (-1) with acetonitrile-0.1% formic acid water as the mobile phase. The flow rate is 1.0 mL 路min ~ (-1), the column temperature is 25 鈩，

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