本文關(guān)鍵詞：斜投影和空間夾角判據(jù)結(jié)合紫外光譜分析滴眼液中苯扎氯銨　出處：《分析測試學(xué)報》2016年03期 　論文類型：期刊論文

  更多相關(guān)文章： 斜投影 空間夾角判據(jù) 高效液相色譜-紫外光譜聯(lián)用 苯扎氯銨 滴眼液

【摘要】：采用紫外光譜,將斜投影(OP)與空間夾角判據(jù)(SAC)相結(jié)合,通過十二烷基二甲基芐基氯化銨(1227)含量反映苯扎氯銨(BAC)含量的方式建立了一種快速檢測滴眼液中BAC的新方法。首先采用斜投影提取BAC混合物中1227的純信號,以提取的光譜信號與對應(yīng)濃度值進行最小二乘擬合,建立標(biāo)準(zhǔn)光譜矩陣;再通過高效液相色譜-紫外光譜聯(lián)用獲取滴眼液本底光譜矩陣;最后根據(jù)空間夾角判據(jù)測定滴眼液樣品中1227的含量,并以此反映BAC的含量。結(jié)果顯示:在1~20μg/m L濃度范圍內(nèi),斜投影提取的1227純信號與對應(yīng)濃度呈良好線性關(guān)系(r0.999 6),方法的檢測結(jié)果與高效液相色譜法接近,相對誤差不大于2.3%,相對標(biāo)準(zhǔn)偏差(RSD,n=6)不大于2.8%,回收率為99.6%~101.8%。該方法采用多波長紫外光譜直接定量BAC,無需復(fù)雜的樣品前處理,定量時間短,分析效率高,穩(wěn)健性好,可推廣應(yīng)用于其他領(lǐng)域。
[Abstract]:In this paper, the oblique projection opp) is combined with the spatial angle criterion SAC) by ultraviolet spectroscopy. The content of 12 alkyl dimethyl benzyl ammonium chloride (BAC) was reflected by the content of benzalkylammonium chloride (BAC). A new method for rapid detection of BAC in eye drops was established by using the method of content. Firstly, the pure signal of 1227 in BAC mixture was extracted by oblique projection. The standard spectral matrix is established by least square fitting between the extracted spectral signal and the corresponding concentration. The background spectral matrix of eye drops was obtained by high performance liquid chromatography-UV spectroscopy. Finally, the content of 1227 in the eye drops was determined according to the spatial angle criterion, and the content of BAC was reflected. The results showed that the concentration of 1227 was in the range of 1 ~ 20 渭 g / mL. There was a good linear relationship between 1227 pure signal extracted by oblique projection and the corresponding concentration. The detection result of the method was close to that of high performance liquid chromatography (HPLC), and the relative error was not more than 2.3%. The relative standard deviation (RSD) was not more than 2.8, and the recovery was 99.6% and 101.8%. The multiwavelength UV spectrum was used to determine BAC directly. Without complex sample pretreatment, the quantitative time is short, the analysis efficiency is high, the stability is good, and it can be applied to other fields.
【作者單位】： 廣西科技大學(xué)生物與化學(xué)工程學(xué)院;廣西糖資源綠色加工重點實驗室;
【基金】：廣西科技計劃項目(桂科攻1355010-15) 柳州市科技計劃項目(2015J030201)
【分類號】：R927;O657.3
【正文快照】： 苯扎氯銨(BAC)是滴眼液中一種常用的防腐劑,使用添加過量BAC的滴眼液會對眼內(nèi)細(xì)胞造成傷害[1],即使其濃度低于0.001%,經(jīng)常使用或使用超過30 min以上也會引起角膜上皮細(xì)胞功能障礙[2]。目前BAC的檢測方法主要為高效液相色譜(HPLC)法[3-6],HPLC能有效測定BAC含量,但其色譜峰易拖

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