【摘要】：目的觀察不同程度間歇性低氧后大鼠不同腦區(qū)Bax、Bcl-2表達(dá)變化,探討阻塞性睡眠呼吸暫停低通氣綜合征(OSAHS)神經(jīng)損傷機(jī)制。方法 30只雄性SD大鼠隨機(jī)分成對(duì)照組、輕度間歇低氧組、重度間歇低氧組各10只。對(duì)照組暴露于空氣中,間歇低氧組分別暴露于不同低氧條件下(100 ml/L和50 ml/L,暴露時(shí)間每天8 h,持續(xù)時(shí)間2 w和8 w)。光鏡觀察額葉皮質(zhì)、紋狀體、海馬區(qū)和小腦神經(jīng)細(xì)胞結(jié)構(gòu)變化,免疫組化檢測(cè)上述區(qū)域Bax、Bcl-2表達(dá)變化。結(jié)果與對(duì)照組比較,隨低氧時(shí)間的延長,兩組間歇性低氧組大鼠額葉皮質(zhì)、紋狀體、海馬區(qū)和小腦神經(jīng)細(xì)胞區(qū)死亡神經(jīng)元密度增高;Bcl-2/Bax比值降低(P0.05);上述變化在重度間歇性低氧組最為顯著(P0.05);輕度間歇性低氧組各腦區(qū)死亡神經(jīng)元密度、Bcl-2/Bax比值變化差異顯著,以額葉皮質(zhì)和海馬最明顯(P0.05);重度間歇性低氧組各腦區(qū)死亡神經(jīng)元密度、Bcl-2/Bax比值變化差異顯著,以皮質(zhì)、海馬和紋狀體區(qū)最明顯。結(jié)論間歇性低氧可引起不同腦區(qū)Bcl-2和Bax差異性表達(dá)、神經(jīng)細(xì)胞差異性丟失;皮質(zhì)、海馬和紋狀體對(duì)間歇性低氧損傷更敏感。
[Abstract]:Objective to observe the changes of Bax,Bcl-2 expression in different brain regions of rats after intermittent hypoxia and to explore the mechanism of (OSAHS) nerve injury in obstructive sleep apnea hypopnea syndrome. Methods 30 male SD rats were randomly divided into control group (n = 10), mild intermittent hypoxia group (n = 10) and severe intermittent hypoxia group (n = 10). The control group was exposed to air, and the intermittent hypoxia group was exposed to different hypoxia conditions (100 ml/L and 50 ml/L, exposure time 8 h, duration 2 w and 8 w)., respectively). The structural changes of nerve cells in frontalis cortex, striatum, hippocampus and cerebellum were observed by light microscope, and the expression of Bax,Bcl-2 in these areas was detected by immunohistochemistry. Results compared with the control group, with the prolongation of hypoxia time, the density of dead neurons in the frontolobe cortex, striatum, hippocampus and cerebellar nerve cell area of the two groups increased, and the ratio of Bcl-2/Bax decreased (P 0.05). The above changes were most significant in the severe intermittent hypoxia group (P 0.05), and the death neuron density and Bcl-2/Bax ratio in each brain area were significantly different in the mild intermittent hypoxia group, especially in the frontolobe cortex and hippocampus (P 0.05). In severe intermittent hypoxia group, the density of dead neurons and the ratio of Bcl-2/Bax in each brain area were significantly different, especially in cortex, hippocampus and striatum. Conclusion intermittent hypoxia can induce the differential expression of Bcl-2 and Bax and the loss of nerve cells in different brain regions, and the cortex, hippocampus and striatum are more sensitive to intermittent hypoxia injury.
【作者單位】： 河北聯(lián)合大學(xué)康復(fù)醫(yī)學(xué)院;河北聯(lián)合大學(xué)附屬醫(yī)院;
【基金】：河北省科技廳資助項(xiàng)目(No.09276103D-11)
【分類號(hào)】：R766

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