【摘要】：研究目的： 1.研究膜型1基質(zhì)金屬蛋白酶(MT1-MMP)在不同視網(wǎng)膜前膜(ERM)組織中的表達(dá)差異。 2.研究MT1-MMP在視網(wǎng)膜前膜新生血管內(nèi)皮細(xì)胞中的表達(dá)及分布情況。 研究方法： 1.收集增殖性糖尿病視網(wǎng)膜病變(PDR),增殖性玻璃體視網(wǎng)膜病變(PVR)及特發(fā)性黃斑前膜(IERMM)患者的ERM標(biāo)本及臨床資料。 2.通過免疫組織化學(xué)染色,觀察MT1-MMP在不同ERM中的表達(dá)強弱,并通過圖像分析軟件(Image Pro Plus6.0)分析其累計光密度(IOD)值。 3.通過免疫熒光雙重標(biāo)記染色,以CD34標(biāo)記ERM中新生血管內(nèi)皮細(xì)胞,觀察MT1-MMP在內(nèi)皮細(xì)胞中的表達(dá)及分布。 研究結(jié)果： 1.共收集33例ERM標(biāo)本,其中PDR患者22例,非PDR患者11例(PVR患者7例,IERMM4例)。 2.在PDR和非PDR患者的ERM標(biāo)本中,MT1-MMP表達(dá)陽性率分別為77.3%(17/22)和18.2%(2/11)(p=0.002)；在PDR和非PDR患者的ERM標(biāo)本中,MT1-MMP表達(dá)的平均IOD值分別為5767.36±2505.03和1728.73±717.32(p=0.000)。 3.在PDR患者的ERM標(biāo)本中,MT1-MMP與CD34免疫熒光染色陽性,分布較一致,均位于細(xì)胞膜上。在非PDR患者的ERM標(biāo)本中,MT1-MMP與CD34免疫熒光染色陰性。 研究結(jié)論： 1.與對照組相比,糖尿病視網(wǎng)膜病變視網(wǎng)膜前膜顯著高表達(dá)MT1-MMP, MT1-MMP可能在糖尿病視網(wǎng)膜病變增殖膜形成中發(fā)揮一定作用。 2.糖尿病視網(wǎng)膜病變的視網(wǎng)膜前膜中含有較多新生血管成分,MT1-MMP表達(dá)在糖尿病視網(wǎng)膜病變視網(wǎng)膜前膜新生血管內(nèi)皮細(xì)胞的細(xì)胞膜上,MT1-MMP可能在糖尿病視網(wǎng)膜病變視網(wǎng)膜前膜的新生血管形成中發(fā)揮一定作用。
[Abstract]:Research purposes: 1. To study the expression of membrane type 1 matrix metalloproteinases (MT1-MMP) in different (ERM) tissues. 2. To study the expression and distribution of MT1-MMP in endothelial cells of epiretinal neovascularization. Methods of research: 1. ERM specimens and clinical data of patients with proliferative diabetic retinopathy (PDR), proliferative vitreoretinopathy (PVR) and idiopathic macular anterior membrane (IERMM) were collected. 2. The expression of MT1-MMP in different ERM was observed by immunohistochemical staining, and the cumulative optical density (IOD) was analyzed by image analysis software (Image Pro Plus6.0). 3. The expression and distribution of MT1-MMP in ERM were observed by double immunofluorescence staining, and the expression and distribution of MT1-MMP in endothelial cells were observed by using CD34 to label the neovascularization endothelial cells. Research findings: 1. 33 cases of ERM were collected, including 22 cases of PDR and 11 cases of non-PDR (7 cases of PVR and 4 cases of IERMM). 2. The positive rates of MT1-MMP expression in ERM samples from PDR and non-PDR patients were 77.3% (17 / 22) and 18.2% (2 / 11) respectively (p = 0.002). In ERM specimens from PDR and non-PDR patients, the average IOD values of MT1-MMP expression were 5767.36 鹵2505.03 and 1728.73 鹵717.32 (p < 0. 000), respectively. 3. In the ERM samples of PDR patients, MT1-MMP and CD34 immunofluorescence staining were positive, the distribution was consistent, both of them were located on the cell membrane. MT1-MMP and CD34 immunofluorescence staining was negative in ERM samples from non-PDR patients. Research conclusions: 1. Compared with the control group, the high expression of MT1-MMP, MT1-MMP in the epiretinal membrane of diabetic retinopathy may play a role in the formation of proliferative membrane in diabetic retinopathy. 2. The epiretinal membrane of diabetic retinopathy contains more neovascularization components, and MT1-MMP is expressed on the membrane of neovascularization endothelial cells in diabetic retinopathy. MT1-MMP may play a role in the neovascularization of diabetic retinopathy.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：博士
【學(xué)位授予年份】：2010
【分類號】：R587.2;R774.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 方玉蘭;唐蘭芬;;早產(chǎn)兒視網(wǎng)膜病的發(fā)病機制與治療[J];廣東醫(yī)學(xué)院學(xué)報;2012年04期

2 穆媛;莊映輝;;環(huán)孢菌素A對鏈脲佐菌素誘導(dǎo)的糖尿病大鼠腎臟組織中MIP-1α mRNA表達(dá)的影響[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2012年04期

3 黃龍;何煜;廖婷婷;張琰;;糖尿病腎病患者的胱抑素C水平及其臨床意義[J];廣西醫(yī)學(xué);2013年05期

4 蘭天;常秀亭;勾紅菊;吳騰;王麗京;黃河清;;SphK1-S1P信號通路的激活參與糖尿病大鼠腎損傷的研究[J];重慶醫(yī)學(xué);2013年22期

5 馮靈香;李麗;楊力;;芪明顆粒聯(lián)合依那普利治療糖尿病腎病45例的回顧性分析[J];貴陽中醫(yī)學(xué)院學(xué)報;2013年05期

6 周濤;鮑遠(yuǎn)程;謝道俊;鮑婷婷;張娟;;從通腑化瘀論治肝豆?fàn)詈俗冃约捌涓卫w維化[J];中醫(yī)藥臨床雜志;2013年11期

7 Chen-Yuan Gong;Bin Lu;Qian-Wen Hu;Li-Li Ji;;Streptozotocin induced diabetic retinopathy in rat and the expression of vascular endothelial growth factor and its receptor[J];International Journal of Ophthalmology(English Edition);2013年05期

8 高岑;馬躍榮;史書龍;陳秋;;蛻皮甾酮對高糖培養(yǎng)大鼠腎小球系膜細(xì)胞增殖及其細(xì)胞外基質(zhì)含量的影響[J];瀘州醫(yī)學(xué)院學(xué)報;2011年04期

9 姚芳;李志紅;閆U，

本文編號：2431433