國產(chǎn)親水性丙烯酸人工晶狀體的生物相容性研究
發(fā)布時間：2019-01-20 18:36
【摘要】： 目的： 軟性人工晶狀體(Intraocular Lens IOL)是目前白內(nèi)障術(shù)中使用的主流人工晶狀體,而我國軟性IOL的研發(fā)尚處于起步階段。本研究擬對天津泰達(dá)生物科技有限公司研發(fā)的軟性親水性丙烯酸IOL進(jìn)行體外的細(xì)胞毒性檢測,并將其植入兔眼內(nèi)以綜合評價其生物相容性。 方法： 分為體外和體內(nèi)實(shí)驗(yàn)兩部分。 1.體外,選擇親水性丙烯酸、疏水性丙烯酸和硅凝膠三種軟性材料的IOL進(jìn)行生物相容性和細(xì)胞毒性的觀察和比較。分別提取人外周血的血小板、中性粒細(xì)胞,小鼠腹腔巨噬細(xì)胞、人眼結(jié)膜囊成纖維細(xì)胞置于三種IOL表面并培養(yǎng),觀察其細(xì)胞粘附生長的數(shù)量與狀態(tài)。 2.選擇表皮葡萄球菌標(biāo)準(zhǔn)菌株對以上三種軟性IOL及聚甲基丙烯酸甲酯(polymethyl methacrylate PMMA)IOL、肝素修飾PMMA IOL進(jìn)行細(xì)菌粘附的比較。 3.體內(nèi),依照國家標(biāo)準(zhǔn)和國家醫(yī)藥局發(fā)布的《眼內(nèi)IOL》標(biāo)準(zhǔn),選擇已臨床應(yīng)用的進(jìn)口親水性丙烯酸IOL作為對照IOL,進(jìn)行家兔眼內(nèi)植入的初步研究。選擇日本大耳白兔26只,分為4組：第一組為正常對照,2只兔,第二組16只兔,行雙眼超聲乳化晶狀體吸除后,一眼植入實(shí)驗(yàn)IOL,另一眼植入對照IOL；第三組4只兔,晶狀體乳化吸除后一眼植入實(shí)驗(yàn)IOL,一眼不植IOL；第四組4只眼,晶狀體乳化吸除后一眼植入對照IOL,另一眼不植入IOL,共計(jì)各有20枚實(shí)驗(yàn)和對照IOL植入兔眼內(nèi)。 4.分別于術(shù)后1周、1月、3月抽取兔眼前房水進(jìn)行白細(xì)胞計(jì)數(shù),并行裂隙燈檢察,觀測眼前節(jié)的反應(yīng)。于術(shù)后1月、3月分別處死第二組的5只兔,取出IOL進(jìn)行掃描電鏡檢查,并在同時期各處死第三、四組的2只兔,摘除眼球,行兔眼前、后節(jié)的病理觀察。 結(jié)果： 1.體外,血小板和中性粒細(xì)胞在親水性丙烯酸IOL表面粘附的最少,明顯少于疏水性丙烯酸和硅凝膠IOL；小鼠腹腔巨噬細(xì)胞在親水性丙烯酸IOL表面的粘附少于疏水性丙烯酸IOL,而與硅凝膠IOL無顯著性差異；人結(jié)膜囊成纖維細(xì)胞在疏水性丙烯酸IOL表面生長最密集,親水性丙烯酸IOL表面的成纖維細(xì)胞粘附的較少,但形態(tài)健康。 2.表皮葡萄球菌在親水性丙烯酸IOL上粘附的最少,與肝素修飾的IOL相當(dāng),在硅凝膠IOL表面粘附的最多。 3.兔眼內(nèi),實(shí)驗(yàn)國產(chǎn)親水性丙烯酸IOL植入術(shù)后7天,房水白細(xì)胞計(jì)數(shù)較對照組多,其余各觀察時間(30天、90天)的房水白細(xì)胞計(jì)數(shù)兩組間無顯著性差異。國產(chǎn)親水性丙烯酸IOL植入后角膜水腫、前房滲出及IOL表面附著物情況的裂隙燈檢查,與進(jìn)口親水性丙烯酸IOL相比均未見明顯區(qū)別。 4.IOL取出后掃描電鏡顯示,術(shù)后1個月,國產(chǎn)親水性丙烯酸IOL表面可見少量蛋白膜,巨噬細(xì)胞成團(tuán)分布,術(shù)后3個月,表面蛋白膜縮小,巨噬細(xì)胞單個分布,與進(jìn)口對照組之間無顯著性區(qū)別。 5.兔眼摘除后,兩組眼前節(jié)組織病理學(xué)檢查均表現(xiàn)為：術(shù)后1個月,前房內(nèi)少量滲出物,房角開放,術(shù)后3個月,滲出吸收,房角仍大部分開放,視網(wǎng)膜組織各層結(jié)構(gòu)大致正常。 結(jié)論： 1.國產(chǎn)親水性丙烯酸IOL在體外表現(xiàn)為較低的細(xì)胞粘附和細(xì)菌粘附,無細(xì)胞毒性。 2.兔眼內(nèi),國產(chǎn)親水性丙烯酸IOL與進(jìn)口對照IOL相比,同樣具有良好的葡萄膜相容性和較好的囊膜相容性。
[Abstract]:Purpose: The soft intraocular lens (IOL) is the mainstream intraocular lens used in the current cataract operation, and the development of the soft IOL in China is still in the beginning In this study, the soft hydrophilic acrylic IOL developed by Tianjin Taida Biotech Co., Ltd. was tested for cytotoxicity in vitro, and it was implanted in rabbit eyes to evaluate its biological activity. Compatibility. Method: minutes in vitro and in vivo, two parts of soft materials of hydrophilic acrylic acid, hydrophobic acrylic acid and silicon gel are selected to be produced, The human peripheral blood platelet, the neutrophils, the mouse peritoneal macrophages and the human conjunctival sac fibroblasts are respectively extracted to the surface of the three IOLs and cultured, the number and the status of the cell adhesion growth were observed. A comparison of the bacterial adhesion of the prime-modified PMMA IOL. In vivo, the clinically applied imported hydrophilic acrylic acid I was selected in accordance with the National Standards and the <-eye IOL> criteria issued by the National Institute of Medicine. OOL was used as a control IOL for the initial study of the in-eye implantation of rabbit. 26 rabbits were selected as normal control, 2 rabbits and 16 rabbits in the second group. After the two eyes of the two eyes were absorbed and removed, the experimental IOL was implanted at one glance and the control IOL was implanted at another glance; and Four rabbits were implanted in three groups, the lens was emulsified and the IOL was implanted at a glance, and the IOL was not implanted at a glance; the fourth group was 4 eyes, the lens was emulsified and the control IOL was implanted at a glance, and the other one was not implanted A total of 20 experimental and control IOLs were implanted in the rabbit eye. 4. The rabbit eyes were extracted 1 week, 1 month and 3 months after the operation, respectively. White blood cell count and parallel slit lamp were performed in the anterior chamber to observe the reaction of the anterior segment. In January and March of the operation, 5 rabbits of the second group were sacrificed, and the IOL was taken out for scanning electron microscope and the patients were sacrificed at the same time. third, The results were as follows: 1. In vitro, the adhesion of platelets and neutrophils to the surface of the hydrophilic acrylic IOL was the least, significantly less than that of the hydrophobic acrylic and silicone IOLs.; the adhesion of the mouse peritoneal macrophages to the surface of the hydrophilic acrylic IOL was less than that of the hydrophobic acrylic IOL and no significant difference with the silicone IOLs; the human conjunctival sac fibroblasts were in the hydrophobic acrylic IOL table the surface growth was the most dense, the surface of the hydrophilic acrylic IOL had less adhesion, but the morphology was healthy. The minimum amount of adhesion to the acrylic IOL, comparable to the heparin-modified IOL, was the most adhesive on the surface of the silicone IOL. 3. In the rabbit eye, 7 days after the implantation of the home-made hydrophilic acrylic IOL, the aqueous white There was no significant difference between the two groups in the control group, and the remaining observation time (30 days, 90 days) showed no significant difference between the two groups. The domestic hydrophilic acrylic IOL was implanted with corneal edema. The examination of the slit lamp of the exudation of the anterior chamber and the attachment of the surface of the IOL was not significantly different from that of the imported hydrophilic acrylic IOL. 4. The scanning electron microscope showed that the surface of the domestic hydrophilic acrylic IOL could be seen with a small amount of protein film after the operation for 1 month after the IOL was taken out. and after 3 months of operation, the surface protein membrane was reduced, the single distribution of the macrophages, and no significant difference between the control group and the control group. 1 month There was a small amount of exudate in the front room, the angle of the room was open, 3 months after the operation, the effusion was absorbed, the angle of the room was still largely open, and the retina The structure of each layer of the tissue is generally normal. Conclusion: 1. The domestic hydrophilic acrylic IOL is shown to be low in cell adhesion and bacteria in vitro.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2010
【分類號】：R779.66
本文編號：2412293
[Abstract]:Purpose: The soft intraocular lens (IOL) is the mainstream intraocular lens used in the current cataract operation, and the development of the soft IOL in China is still in the beginning In this study, the soft hydrophilic acrylic IOL developed by Tianjin Taida Biotech Co., Ltd. was tested for cytotoxicity in vitro, and it was implanted in rabbit eyes to evaluate its biological activity. Compatibility. Method: minutes in vitro and in vivo, two parts of soft materials of hydrophilic acrylic acid, hydrophobic acrylic acid and silicon gel are selected to be produced, The human peripheral blood platelet, the neutrophils, the mouse peritoneal macrophages and the human conjunctival sac fibroblasts are respectively extracted to the surface of the three IOLs and cultured, the number and the status of the cell adhesion growth were observed. A comparison of the bacterial adhesion of the prime-modified PMMA IOL. In vivo, the clinically applied imported hydrophilic acrylic acid I was selected in accordance with the National Standards and the <-eye IOL> criteria issued by the National Institute of Medicine. OOL was used as a control IOL for the initial study of the in-eye implantation of rabbit. 26 rabbits were selected as normal control, 2 rabbits and 16 rabbits in the second group. After the two eyes of the two eyes were absorbed and removed, the experimental IOL was implanted at one glance and the control IOL was implanted at another glance; and Four rabbits were implanted in three groups, the lens was emulsified and the IOL was implanted at a glance, and the IOL was not implanted at a glance; the fourth group was 4 eyes, the lens was emulsified and the control IOL was implanted at a glance, and the other one was not implanted A total of 20 experimental and control IOLs were implanted in the rabbit eye. 4. The rabbit eyes were extracted 1 week, 1 month and 3 months after the operation, respectively. White blood cell count and parallel slit lamp were performed in the anterior chamber to observe the reaction of the anterior segment. In January and March of the operation, 5 rabbits of the second group were sacrificed, and the IOL was taken out for scanning electron microscope and the patients were sacrificed at the same time. third, The results were as follows: 1. In vitro, the adhesion of platelets and neutrophils to the surface of the hydrophilic acrylic IOL was the least, significantly less than that of the hydrophobic acrylic and silicone IOLs.; the adhesion of the mouse peritoneal macrophages to the surface of the hydrophilic acrylic IOL was less than that of the hydrophobic acrylic IOL and no significant difference with the silicone IOLs; the human conjunctival sac fibroblasts were in the hydrophobic acrylic IOL table the surface growth was the most dense, the surface of the hydrophilic acrylic IOL had less adhesion, but the morphology was healthy. The minimum amount of adhesion to the acrylic IOL, comparable to the heparin-modified IOL, was the most adhesive on the surface of the silicone IOL. 3. In the rabbit eye, 7 days after the implantation of the home-made hydrophilic acrylic IOL, the aqueous white There was no significant difference between the two groups in the control group, and the remaining observation time (30 days, 90 days) showed no significant difference between the two groups. The domestic hydrophilic acrylic IOL was implanted with corneal edema. The examination of the slit lamp of the exudation of the anterior chamber and the attachment of the surface of the IOL was not significantly different from that of the imported hydrophilic acrylic IOL. 4. The scanning electron microscope showed that the surface of the domestic hydrophilic acrylic IOL could be seen with a small amount of protein film after the operation for 1 month after the IOL was taken out. and after 3 months of operation, the surface protein membrane was reduced, the single distribution of the macrophages, and no significant difference between the control group and the control group. 1 month There was a small amount of exudate in the front room, the angle of the room was open, 3 months after the operation, the effusion was absorbed, the angle of the room was still largely open, and the retina The structure of each layer of the tissue is generally normal. Conclusion: 1. The domestic hydrophilic acrylic IOL is shown to be low in cell adhesion and bacteria in vitro.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2010
【分類號】：R779.66
【參考文獻(xiàn)】
相關(guān)期刊論文 前6條
1 王桂琴;彭秀軍;顧漢卿;;表面修飾硅凝膠人工晶狀體植入兔眼的生物相容性[J];國際眼科雜志;2006年01期
2 袁佳琴,孫慧敏,徐延山,郭紅玉,王桂琴,顧漢卿,祁明信,黃秀榕;氟-肝素表面修飾人工晶狀體的實(shí)驗(yàn)研究[J];眼科新進(jìn)展;2003年03期
3 柯曉云;自由基與眼部炎癥（一）[J];眼科新進(jìn)展;1994年01期
4 黃蒂;施玉英;;Acrysof ReSTOR多焦點(diǎn)人工晶狀體臨床應(yīng)用的初步報(bào)告[J];眼科;2006年05期
5 楊方耀，劉業(yè)滋，李紹珍，林云朝;后房型人工晶體植入術(shù)后晶體表面細(xì)胞反應(yīng)的實(shí)驗(yàn)研究[J];眼科研究;1995年02期
6 王桂琴,袁佳琴,顧漢卿,孫慧敏,徐延山,黃秀榕,祁明信,李志雄,周建華,范春梅,陳旋武;氟-肝素表面修飾的人工晶狀體植入獼猴眼內(nèi)的實(shí)驗(yàn)研究[J];中華眼科雜志;2000年02期
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