【摘要】： 【目的】:探討miR-21及PDCD4在喉鱗癌中的表達情況,及分析miR-21與其靶基因PDCD4在喉鱗癌中的相關(guān)研究。 【方法】:(1)應(yīng)用半定量RT-PCR檢測PDCD4在喉鱗癌組織(38例)及癌旁正常組織(38例)中的表達,并結(jié)合臨床資料分析PDCD4與腫瘤臨床病理學(xué)特性的相關(guān)性。 (2)運用TaqMan? real-time RT-PCR方法檢測miR-21在喉鱗癌組織(38例)及癌旁正常組織(38例)中的表達情況。 【結(jié)果】:(1)半定量RT-PCR檢測結(jié)果顯示,PDCD4 mRNA在喉鱗癌組織中下調(diào)率達86.8%,同癌旁正常組織相比,喉鱗癌組織中PDCD4 mRNA表達明顯下調(diào)(P0.05);PDCD4 mRNA表達與腫瘤的分化程度、TNM分期及淋巴轉(zhuǎn)移密切相關(guān)(P0.05),在腫瘤的不同解剖分區(qū)中表達無顯著差異(P0.05);在不同分化程度喉鱗癌組織之間PDCD4 mRNA表達存在明顯差異,隨著分化程度下降,表達明顯下調(diào);有淋巴結(jié)轉(zhuǎn)移者的PDCD4 mRNA表達顯著低于無淋巴結(jié)轉(zhuǎn)移者。 (2)配對樣本W(wǎng)ilcoxon符號秩檢驗,發(fā)現(xiàn)miR-21的數(shù)據(jù)有統(tǒng)計學(xué)意義(P0.05), miR-21在喉鱗癌病例樣本中明顯高表達。 【結(jié)論】: miR-21與PDCD4的表達呈負相關(guān)性,miR-21與PDCD4在喉鱗癌中的異常表達可能與喉鱗癌的發(fā)生、發(fā)展有關(guān),miR-21可能抑制PDCD4的表達,減弱PDCD4抑制腫瘤細胞生長的能力,從而促進腫瘤的發(fā)生和發(fā)展。
[Abstract]:Objective: to investigate the expression of miR-21 and PDCD4 in laryngeal squamous cell carcinoma (LSCC) and to analyze the correlation between miR-21 and its target gene PDCD4 in laryngeal squamous cell carcinoma (LSCC). Methods: (1) Semi-quantitative RT-PCR was used to detect the expression of PDCD4 in laryngeal squamous cell carcinoma (LSCC) tissues (38 cases) and adjacent normal tissues (38 cases). The correlation between PDCD4 and clinicopathological characteristics was analyzed with clinical data. (2) Application of TaqMan? Real-time RT-PCR method was used to detect the expression of miR-21 in laryngeal squamous cell carcinoma (LSCC) tissues (38 cases) and adjacent normal tissues (38 cases). [results]: (1) the results of semi-quantitative RT-PCR showed that the down-regulation rate of PDCD4 mRNA in laryngeal squamous cell carcinoma (LSCC) was 86.8%, and the expression of PDCD4 mRNA in laryngeal squamous cell carcinoma (LSCC) was significantly down-regulated compared with the adjacent normal tissue (P0.05). The expression of PDCD4 mRNA was closely related to the degree of tumor differentiation, TNM stage and lymphatic metastasis (P0.05), but there was no significant difference in the expression of PDCD4 mRNA in different anatomic regions of the tumor (P0.05). The expression of PDCD4 mRNA in laryngeal squamous cell carcinoma (LSCC) with different degree of differentiation was significantly different, and the expression of PDCD4 mRNA was significantly lower in patients with lymph node metastasis than in those without lymph node metastasis. (2) the Wilcoxon sign rank test of paired samples showed that the data of miR-21 was statistically significant (P0.05), and miR-21 was significantly overexpressed in laryngeal squamous cell carcinoma (LSCC) samples. [conclusion] there is a negative correlation between the expression of miR-21 and PDCD4. The abnormal expression of miR-21 and PDCD4 in laryngeal squamous cell carcinoma may be related to the occurrence and development of laryngeal squamous cell carcinoma. MiR-21 may inhibit the expression of PDCD4 and weaken the ability of PDCD4 to inhibit the growth of tumor cells. So as to promote the occurrence and development of tumors.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.65

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