地塞米松對實驗性過敏性鼻炎小鼠血清和鼻中隔黏膜TGF-β表達的影響
發(fā)布時間：2018-12-12 01:55
【摘要】： 目的：探討地塞米松(Dexamethasone,DEX)對實驗性過敏性鼻炎小鼠模型血清和鼻中隔黏膜中轉(zhuǎn)化生長因子-β(transforming growth factor-β,TGF-β)表達的影響。方法：4-6周BALB/c小鼠40只,隨機分為四組：正常對照A組、模型對照B組、治療C組及治療D組。以卵清蛋白(ovalbumin,OVA)作為致敏原；致敏實驗小鼠、建立過敏性鼻炎小鼠模型。分析比較小鼠過敏癥狀評分,采用蘇木素-伊紅(hematoxylin-eosin,HE)染色法分析比較實驗小鼠鼻中隔黏膜嗜酸性粒細胞(eosinophils,EOS)浸潤程度。應(yīng)用免疫組化法分析實驗小鼠鼻中隔黏膜上皮細胞及炎性細胞內(nèi)TGF-β表達情況。采用酶聯(lián)免疫吸附法(ELISA)檢測并分析比較實驗小鼠外周血清中TGF-β及IgE的水平。應(yīng)用spss11.5軟件對實驗數(shù)據(jù)進行統(tǒng)計學(xué)分析：實驗數(shù)據(jù)均以x±s表示,采用單因素方差分析,組間比較采用t檢驗。檢驗水準(zhǔn)取a=0.05,以P0.05為差異有統(tǒng)計學(xué)意義。 結(jié)果：B組小鼠鼻中隔黏膜組織嗜酸性粒細胞浸潤顯著高于A組(P0.01)；C組小鼠過敏癥狀評分、鼻中隔黏膜嗜酸性粒細胞浸潤、血清IgE水平較B組顯著降低(P0.01),其血清TGF-β水平較B組顯著增高(P0.01)；D組小鼠僅鼻中隔黏膜嗜酸性粒細胞浸潤低于B組(P0.01)；其過敏癥狀評分、血清IgE水平及血清TGF-β水平與B組比較無顯著差異(P0.05)。A組小鼠鼻中隔黏膜未見TGF-β表達,B組小鼠鼻中隔黏膜內(nèi)TGF-β表達明顯,兩個治療組小鼠鼻中隔黏膜TGF-β表達均低于B組(P0.01)。 結(jié)論：地塞米松根據(jù)給藥時間不同可分別抑制不同時相的鼻變態(tài)反應(yīng)。基礎(chǔ)致敏前使用地塞米松可抑制速發(fā)相和遲發(fā)相過敏反應(yīng)；局部激發(fā)前使用地塞米松僅能抑制遲發(fā)相過敏反應(yīng)。地塞米松可能至少通過兩種機制影響TGF-β的表達,從而在過敏性鼻炎的治療過程中發(fā)揮作用。一是通過上調(diào)外周血清TGF-β的水平從而抑制過敏反應(yīng)的發(fā)生；二是通過降低鼻黏膜上皮細胞及嗜酸性粒細胞TGF-β的表達,抑制炎癥細胞的增殖和趨化,從而抑制鼻部過敏性炎性反應(yīng)的發(fā)生,最終可有效遏制鼻黏膜發(fā)生組織重塑。
[Abstract]:Aim: to investigate the effect of dexamethasone (Dexamethasone,DEX) on the expression of transforming growth factor 尾 (transforming growth factor- 尾 (TGF- 尾) in serum and septum mucosa of experimental allergic rhinitis mice. Methods: forty BALB/c mice at 4-6 weeks were randomly divided into four groups: normal control group A, model control group B, treatment group C and treatment group D. The mouse model of allergic rhinitis was established by using ovalbumin (ovalbumin,OVA) as allergen and sensitizing mice. The allergic symptom score of mice was analyzed and the infiltration of eosinophil (eosinophils,EOS) in nasal septum of experimental mice was analyzed by hematoxylin eosinophil (hematoxylin-eosin,HE) staining. The expression of TGF- 尾 in nasal septum mucosal epithelial cells and inflammatory cells was analyzed by immunohistochemical method. Enzyme linked immunosorbent assay (ELISA) was used to detect and compare the levels of TGF- 尾 and IgE in peripheral serum of experimental mice. Spss11.5 software was used to analyze the experimental data. The experimental data were expressed as x 鹵s, single factor analysis of variance and t-test were used in the comparison between groups. The test level was 0. 05, with P 0. 05 as the difference. Results: the infiltration of eosinophils in nasal septum mucosa in group B was significantly higher than that in group A (P0.01). The allergic symptom score, eosinophil infiltration in nasal septum mucosa, serum IgE level and serum TGF- 尾 level in group C were significantly lower than those in group B (P0.01). The eosinophil infiltration in the nasal septum of group D was lower than that in group B (P0.01). There was no significant difference in allergy symptom score, serum IgE level and serum TGF- 尾 level between group B and group B (P0.05). No expression of TGF- 尾 was found in nasal septum mucosa of). A group, but TGF- 尾 expression in nasal septum mucosa of group B mice was significant. The expression of TGF- 尾 in nasal septum mucosa of both groups was lower than that in group B (P0.01). Conclusion: dexamethasone can inhibit nasal hypersensitivity in different phases according to the time of administration. The use of dexamethasone before basic sensitization can inhibit the rapid phase and delayed phase allergic reaction, and the use of dexamethasone before local excitation can only inhibit the delayed phase allergic reaction. Dexamethasone may play a role in the treatment of allergic rhinitis by affecting the expression of TGF- 尾 through at least two mechanisms. One is to inhibit the occurrence of allergic reaction by upregulating the level of TGF- 尾 in peripheral blood. Second, by reducing the expression of TGF- 尾 in nasal epithelial cells and eosinophils, inhibiting the proliferation and chemotaxis of inflammatory cells, thus inhibiting the occurrence of allergic inflammatory reaction in nasal mucosa, which can effectively curb the tissue remodeling of nasal mucosa.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R765.21
本文編號：2373669
[Abstract]:Aim: to investigate the effect of dexamethasone (Dexamethasone,DEX) on the expression of transforming growth factor 尾 (transforming growth factor- 尾 (TGF- 尾) in serum and septum mucosa of experimental allergic rhinitis mice. Methods: forty BALB/c mice at 4-6 weeks were randomly divided into four groups: normal control group A, model control group B, treatment group C and treatment group D. The mouse model of allergic rhinitis was established by using ovalbumin (ovalbumin,OVA) as allergen and sensitizing mice. The allergic symptom score of mice was analyzed and the infiltration of eosinophil (eosinophils,EOS) in nasal septum of experimental mice was analyzed by hematoxylin eosinophil (hematoxylin-eosin,HE) staining. The expression of TGF- 尾 in nasal septum mucosal epithelial cells and inflammatory cells was analyzed by immunohistochemical method. Enzyme linked immunosorbent assay (ELISA) was used to detect and compare the levels of TGF- 尾 and IgE in peripheral serum of experimental mice. Spss11.5 software was used to analyze the experimental data. The experimental data were expressed as x 鹵s, single factor analysis of variance and t-test were used in the comparison between groups. The test level was 0. 05, with P 0. 05 as the difference. Results: the infiltration of eosinophils in nasal septum mucosa in group B was significantly higher than that in group A (P0.01). The allergic symptom score, eosinophil infiltration in nasal septum mucosa, serum IgE level and serum TGF- 尾 level in group C were significantly lower than those in group B (P0.01). The eosinophil infiltration in the nasal septum of group D was lower than that in group B (P0.01). There was no significant difference in allergy symptom score, serum IgE level and serum TGF- 尾 level between group B and group B (P0.05). No expression of TGF- 尾 was found in nasal septum mucosa of). A group, but TGF- 尾 expression in nasal septum mucosa of group B mice was significant. The expression of TGF- 尾 in nasal septum mucosa of both groups was lower than that in group B (P0.01). Conclusion: dexamethasone can inhibit nasal hypersensitivity in different phases according to the time of administration. The use of dexamethasone before basic sensitization can inhibit the rapid phase and delayed phase allergic reaction, and the use of dexamethasone before local excitation can only inhibit the delayed phase allergic reaction. Dexamethasone may play a role in the treatment of allergic rhinitis by affecting the expression of TGF- 尾 through at least two mechanisms. One is to inhibit the occurrence of allergic reaction by upregulating the level of TGF- 尾 in peripheral blood. Second, by reducing the expression of TGF- 尾 in nasal epithelial cells and eosinophils, inhibiting the proliferation and chemotaxis of inflammatory cells, thus inhibiting the occurrence of allergic inflammatory reaction in nasal mucosa, which can effectively curb the tissue remodeling of nasal mucosa.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R765.21
【引證文獻】
相關(guān)期刊論文 前1條
1 胡艷菲;唐方;;過敏性鼻炎動物模型研究進展[J];醫(yī)學(xué)研究生學(xué)報;2012年05期
，本文編號：2373669
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