【摘要】：目的:觀察甲醛對(duì)小鼠變應(yīng)性鼻炎(AR)的影響。方法:48只雄性BALB/c小鼠隨機(jī)分成6組,每組8只,其中A組為正常對(duì)照組;B組為單純AR組;C組為甲醛暴露組;D、E和F組分別為甲醛低、中、高濃度(1.5、3.0、6.0mg/m3)AR組。采集外周血間接酶聯(lián)免疫吸附試驗(yàn)法測(cè)血清中IL-4、IL-10和干擾素γ(IFN-γ)的水平,并取鼻黏膜行蘇木精-伊紅染色觀察鼻黏膜中嗜酸粒細(xì)胞浸潤(rùn)情況。結(jié)果:氣態(tài)甲醛暴露可導(dǎo)致AR小鼠撓鼻和噴嚏次數(shù)增加;B、D、E和F組小鼠外周血中IL-4和IL-10水平較A組明顯升高(P0.05),D、E和F組較C組明顯升高(P0.05);而E、F組IL-4水平較B組明顯升高(P0.05),D、E和F組IL-10水平明顯高于B組(P0.05)。血清中IFN-γ水平B、D、E、F小鼠明顯低于A組,B組血清中IFN-γ水平明顯高于F組而低于C組,與C組相比D、E、F組外周血IFN-γ表達(dá)明顯降低。鼻黏膜蘇木精-伊紅染色顯示甲醛暴露可使AR小鼠鼻黏膜下嗜酸粒細(xì)胞數(shù)量增多。結(jié)論:氣態(tài)甲醛暴露可促進(jìn)Th2細(xì)胞因子表達(dá),加重鼻黏膜中嗜酸粒細(xì)胞炎癥反應(yīng),協(xié)同致敏原加重小鼠AR的癥狀。
[Abstract]:Objective: to observe the effect of formaldehyde on (AR) of allergic rhinitis in mice. Methods: 48 male BALB/c mice were randomly divided into 6 groups with 8 rats in each group: group A was normal control group, group B was simple AR group, group C was formaldehyde exposure group; Group D, E and F were low, medium and high concentrations of formaldehyde (1.5 ~ 3.0 ~ 6.0 mg / m ~ 3) AR group, respectively. The serum levels of IL-4,IL-10 and interferon 緯 (IFN- 緯) were measured by indirect enzyme linked immunosorbent assay (Elisa) in peripheral blood, and the infiltration of eosinophils in nasal mucosa was observed by hematoxylin eosin staining. Results: exposure to gaseous formaldehyde increased the number of nose scratches and sneezes in AR mice. The levels of IL-4 and IL-10 in the peripheral blood of group B and F were significantly higher than those in group A (P0.05), and the levels of IL-4 and IL-10 in groups D and F were significantly higher than those in group C (P0.05). The level of IL-4 in group E and F was significantly higher than that in group B (P0.05). The level of IL-10 in group E and group F was significantly higher than that in group B (P0.05). The levels of IFN- 緯 in serum of group B were significantly lower than those of group A, and the levels of IFN- 緯 in group B were significantly higher than those in group F but lower than those in group C. the expression of IFN- 緯 in peripheral blood of group C was significantly lower than that of group C. Hematoxylin-eosinophore staining showed that formaldehyde exposure increased the number of submucosal eosinophils in AR mice. Conclusion: exposure to gaseous formaldehyde can promote the expression of Th2 cytokines, aggravate the inflammatory reaction of eosinophils in nasal mucosa and aggravate the symptoms of AR in mice.
【作者單位】： 武漢大學(xué)人民醫(yī)院耳鼻咽喉頭頸外科;
【基金】：國(guó)家自然科學(xué)項(xiàng)目(No:81371070) 湖北省自然科學(xué)基金(No:2014CKB495)
【分類(lèi)號(hào)】：R765.21

【共引文獻(xiàn)】

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本文編號(hào)：2302745