【摘要】：目的：檢測血小板反應(yīng)蛋白-1(Thrombonspondin-1 TSP-1)在氧誘導(dǎo)視網(wǎng)膜病變模型小鼠(OIR)視網(wǎng)膜中的表達(dá),探討其在視網(wǎng)膜新生血管形成中的作用。 方法：隨機(jī)選取7日齡C57BL/6J新生小鼠40只,分為模型組(20只)及正常對照組(20只)。模型組小鼠通過高氧誘導(dǎo)的方法建立氧誘導(dǎo)視網(wǎng)膜病變(OIR)的模型。于小鼠出生后第7、9、11日時(shí)兩組各隨機(jī)抽取5只小鼠,取視網(wǎng)膜組織采用RT-PCR法檢測TSP-1mRNA的表達(dá)水平；并于小鼠出生后第11日時(shí)兩組隨機(jī)取5只小鼠,運(yùn)用熒光造影視網(wǎng)膜鋪片對視網(wǎng)膜新生血管進(jìn)行形態(tài)學(xué)觀察。 結(jié)果：(1)熒光造影視網(wǎng)膜鋪片觀察視網(wǎng)膜血管形態(tài)：出生后第11日時(shí),正常組小鼠視網(wǎng)膜鋪片顯示視網(wǎng)膜血管分布呈均勻的網(wǎng)狀結(jié)構(gòu),而模型組小鼠視盤周圍可見大片無灌注區(qū),視網(wǎng)膜大血管擴(kuò)張,僅在周邊見毛細(xì)血管分布,為典型的OIR病理Ⅰ期表現(xiàn)。 (2) RT-PCR檢測TSP-1mRNA的結(jié)果：正常對照組小鼠視網(wǎng)膜組織中TSP-1mRNA表達(dá)水平各時(shí)間點(diǎn)之間表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05),模型組小鼠視網(wǎng)膜組織中TSP-1mRNA表達(dá)水平各時(shí)間點(diǎn)之間表達(dá)差異有統(tǒng)計(jì)學(xué)意義(P0.05)。出生后7日,模型組與對照組小鼠視網(wǎng)膜組織中TSP-1mRNA表達(dá)水平無明顯差異(P0.05)；出生后9日,模型組小鼠視網(wǎng)膜組織中TSP-1mRNA表達(dá)水平下降(P0.05)；出生后11日模型組小鼠視網(wǎng)膜組織中TSP-1mRNA表達(dá)水平明顯低于正常組(P0.05),且較9日時(shí)模型鼠下降。 結(jié)論：在新生小鼠OIR模型視網(wǎng)膜血管生長發(fā)育抑制期,視網(wǎng)膜組織中TSP-1mRNA的表達(dá)逐漸下降,提示TSP-1可能作為負(fù)調(diào)節(jié)因子在早期即參與視網(wǎng)膜新生血管的形成過程。
[Abstract]:Aim: to detect the expression of platelet reactive protein-1 (Thrombonspondin-1 TSP-1) in (OIR) retina of mice with oxygen-induced retinopathy and to explore its role in retinal neovascularization. Methods: forty 7 day old C57BL/6J newborn mice were randomly divided into two groups: the model group (n = 20) and the normal control group (n = 20). The (OIR) model of retinopathy induced by oxygen was established in the model group by hyperoxia induction. Five mice were randomly selected from each group on the 7th and 11th postnatal days, and the expression of TSP-1mRNA was detected by RT-PCR method in the retina of the two groups, and 5 mice in the two groups were randomly selected at the 11th day after birth. The retinal neovascularization was observed by fluorescence angiography. Results: (1) the retinal vascular morphology was observed by fluorescein angiography: at the 11th day after birth, the retina of normal mice showed a uniform reticular distribution of retinal blood vessels. In the model group, a large area of no perfusion was found around the optic disc, and the large retinal vessels were dilated, and the capillary distribution was observed only around the optic disc. (2) the results of TSP-1mRNA detection by RT-PCR: there was no significant difference in the expression of TSP-1mRNA between different time points in normal control group (P0.05). There was significant difference in the expression of TSP-1mRNA between different time points (P0.05). 7 days after birth, there was no significant difference in TSP-1mRNA expression between the model group and the control group (P0.05), but the expression level of TSP-1mRNA in the retinal tissue of the model group decreased 9 days after birth (P0.05). The expression of TSP-1mRNA in the retina of mice in the model group was significantly lower than that in the normal group (P0.05), and decreased after 9 days. Conclusion: the expression of TSP-1mRNA in retinal tissue decreased gradually during the period of retinal vascular growth inhibition in neonatal OIR model of mice, suggesting that TSP-1 may be involved in the formation of retinal neovascularization as a negative regulatory factor in the early stage of retinal neovascularization.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R774.1

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