【摘要】：目的:本實(shí)驗(yàn)主要從鼻內(nèi)翻性乳頭狀瘤(nasal inverted papilloma,NIP)的病理形態(tài)出發(fā),根據(jù)NIP的病理特點(diǎn)及細(xì)胞凋亡與腫瘤發(fā)生的關(guān)系,采用免疫組織化學(xué)方法檢測(cè)細(xì)胞凋亡相關(guān)因子X-連鎖凋亡抑制蛋白(X-linked inhibitor of apoptosis pretein,XIAP)及凋亡蛋白酶活化因子-1(apoptptic protease activating factor-1,Apaf-1)在NIP中的表達(dá)情況并分析其相互關(guān)系。探討細(xì)胞凋亡機(jī)制在NIP發(fā)生發(fā)展中的作用,為NIP的早期臨床診斷及疾病的發(fā)展程度提供新的參考依據(jù),為NIP的治療也提供了新的指導(dǎo)依據(jù)。 方法:采用兔抗人XIAP多克隆抗體和兔抗人Apaf-1多克隆抗體,通過免疫組織化學(xué)技術(shù)檢測(cè)25例NIP組織標(biāo)本中XIAP及Apaf-1的表達(dá);同時(shí)以鼻鱗狀細(xì)胞癌(nasal squamous cell carcinoma, NSCC)及正常下鼻甲(inferior turbinate,IT)組織為對(duì)照組,檢測(cè)21例NSCC存檔蠟塊標(biāo)本及18例IT標(biāo)本中XIAP及Apaf-1的表達(dá),實(shí)驗(yàn)數(shù)據(jù)采用SPSS17.0軟件包X~2檢驗(yàn)及Spearman相關(guān)分析等。 結(jié)果:(1)XIAP在IT、NIP、NSCC中的表達(dá)陽性率分別是16.67%(3/18)、60.00%(15/25)、90.48%(19/21),兩兩相比,差異均有顯著性(P0.05)。(2)Apaf-1在IT、NIP、NSCC中的表達(dá)陽性率分別是94.44%(17/18)、64.00%(16/25)、23.81%(5/21),兩兩相比,其差異均有顯著性(P0.05)。(3)在15例XIAP陽性表達(dá)的NIP中Apaf-1陽性表達(dá)例數(shù)為13例,在Apaf-1陽性表達(dá)的16例NIP病例中XIAP陽性及陰性例數(shù)分別是13例和3例,兩者相比差異具有顯著性(X~2=8.362,P0.05),據(jù)Spearman統(tǒng)計(jì)分析結(jié)果Rs=-0.895,可知NIP中XIAP與Apaf-1的表達(dá)呈負(fù)相關(guān)。 結(jié)論:XIAP及Apaf-1在NIP中表達(dá)介于IT與NSCC之間,表達(dá)率分別呈遞增及遞減趨勢(shì),兩兩相比均具有顯著差異(P0.05),提示XIAP的升高與Apaf-1的降低與腫瘤的發(fā)生發(fā)展有密切關(guān)系。XIAP與Apaf-1在NIP的表達(dá)具有負(fù)相關(guān)性,且在不同的NIP病例中二者的表達(dá)程度不同,提示XIAP異常高表達(dá)與Apaf-1明顯低表達(dá)的病例可能更易惡性變或復(fù)發(fā)。通過對(duì)二者的檢測(cè)可以對(duì)NIP的早期發(fā)現(xiàn),發(fā)展及惡性變等生物學(xué)行為提供依據(jù),從而為有效治療NIP,選用合理的手術(shù)方式提供理論依據(jù)。
[Abstract]:Objective: this study was based on the pathological morphology of nasal inverted papilloma (nasal inverted papilloma,NIP), according to the pathological characteristics of NIP and the relationship between apoptosis and tumorigenesis. The expression of apoptosis-related factor X- linkage inhibitor of apoptosis protein (X-linked inhibitor of apoptosis pretein,XIAP) and apoptotic protease activator 1 (apoptptic protease activating factor-1,Apaf-1 in NIP was detected by immunohistochemical method and the relationship between them was analyzed. To explore the role of apoptosis mechanism in the pathogenesis and development of NIP, to provide a new reference for the early clinical diagnosis and disease development of NIP, as well as a new guiding basis for the treatment of NIP. Methods: using rabbit anti-human XIAP polyclonal antibody and rabbit anti-human Apaf-1 polyclonal antibody, the expression of XIAP and Apaf-1 in 25 cases of NIP were detected by immunohistochemistry. The expression of XIAP and Apaf-1 in 21 cases of NSCC archived wax and 18 cases of IT were detected with (nasal squamous cell carcinoma, NSCC) and normal inferior turbinate (inferior turbinate,IT) as control group. The data were detected by SPSS17.0 software X2 and Spearman correlation analysis. Results: (1) the positive rates of XIAP expression in IT,NIP,NSCC were 16.67% (3 / 18), 60.00% (15 / 25), 90.48% (19 / 21), respectively (P0.05). (2). The positive rates of Apaf-1 in IT,NIP,NSCC were 94.44% (17 / 18), 64.00% (16 / 25), 23.81% (5 / 21), respectively. There were significant differences between them (P0.05). (3). The number of Apaf-1 positive expression in NIP of 15 XIAP positive cases was 13, and the XIAP positive and negative in Apaf-1 positive NIP was 13 and 3, respectively. There was significant difference between the two groups (P 0.05). According to Spearman statistical analysis, the expression of XIAP and Apaf-1 in NIP was negatively correlated with that of XIAP. Conclusion: the expression of XIAP and Apaf-1 in NIP is between IT and NSCC, and the expression rate is increasing and decreasing, respectively. There was significant difference between the two groups (P0.05), which suggested that the increase of XIAP and the decrease of Apaf-1 were closely related to the occurrence and development of tumor. The expression of XIAP and Apaf-1 in NIP was negatively correlated, and the expression degree of XIAP and Apaf-1 in different NIP cases was different. The results suggest that the cases with abnormal high expression of XIAP and obvious low expression of Apaf-1 may be more prone to malignant change or recurrence. The detection of both can provide the basis for the early detection, development and malignant change of NIP, so as to provide theoretical basis for the effective treatment of NIP, with reasonable surgical methods.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R739.62

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 曾化,孔鵬,曾進(jìn),葉章群;腎細(xì)胞癌中X染色體相關(guān)的凋亡抑制蛋白的表達(dá)與細(xì)胞凋亡的關(guān)系[J];臨床泌尿外科雜志;2004年10期

2 菅志遠(yuǎn),李宜雄,李小剛,陳儉云;XIAP在胰腺癌組織中的表達(dá)及其意義[J];中國普通外科雜志;2005年05期

3 李瀟;引起腫瘤細(xì)胞自殺的化合物[J];中國生物化學(xué)與分子生物學(xué)報(bào);2004年04期

4 孫革;孟予城;陳洪杰;張占杰;李小平;劉艷榮;劉立衡;楊樹森;;Ki-67、XIAP在甲狀腺乳頭狀癌中的表達(dá)及相互關(guān)系[J];河北醫(yī)藥;2011年05期

5 孟予城;陳洪杰;楊樹森;孫革;張占杰;李小平;劉艷榮;劉立衡;;Survivin、XIAP在甲狀腺乳頭狀癌組織中表達(dá)的研究[J];河北醫(yī)藥;2011年05期

6 來衛(wèi)東;胡娜;:顧潤國;;凋亡抑制蛋白XIAP的研究進(jìn)展[J];山東醫(yī)學(xué)高等�？茖W(xué)校學(xué)報(bào);2010年06期

7 孫建建;李勝棉;;XIAP表達(dá)與胰腺癌關(guān)系的研究進(jìn)展[J];實(shí)用癌癥雜志;2011年01期

8 白劍英;細(xì)胞凋亡抑制蛋白的研究進(jìn)展[J];中國公共衛(wèi)生;2003年06期

9 楊運(yùn)發(fā);杜洪;王建煒;黃，

本文編號(hào)：2284139