【摘要】：目的：研究高麗紅參對sD大鼠鼓膜內注射慶大霉素引起的耳前庭功能和聽力功能損傷的保護作用。 方法：22只7周齡sD大鼠分為對照組和實驗組,對照組(n=12)：左耳鼓膜內注射慶大霉素(劑量：20ul,濃度：500mg/ml,兩天一次,共七次),不執(zhí)行高麗紅參灌胃。實驗組：注射慶大霉素前7天開始灌胃高麗紅參粉溶液(劑量2ml,500mg/kg,每天一次)；第七次高麗紅參灌胃后,執(zhí)行慶大霉素注射,方法同對照組。慶大霉素注射前進行耳前庭功能和聽力功能檢查,前庭功能檢查方法包括：頭偏實驗,提尾實驗,游泳實驗,動物跑步機測試。聽力檢查方法包括：聽性腦干反應測試。實驗后1天,8天,15天,22天,30天分別進行前庭功能,聽力功能檢查。第30天檢查結束后取大鼠耳蝸前庭器固定,用掃描電鏡,共聚焦顯微鏡觀察耳蝸毛細胞,球囊斑,橢圓囊斑毛細胞。結果進行統(tǒng)計學分析。 結果：(1)功能檢查：實驗前,前庭功能和聽力功能檢查均正常。試驗后第一天對照組：聽閾升高至58.3±9.9dB(16赫茲)和57.1±8.7dB(32赫茲),第三十天為45.8±9.5dB(16赫茲)和42.1±9.1dB(32赫茲)。實驗組：聽閾升高至51.0±10.8dB(16赫茲)和51.0±9.9dB (32赫茲),第三十天為41.0±12.4dB(16赫茲)和37.5±12.9 dB(32赫茲)。結果實驗組均低于對照組,但統(tǒng)計學結果沒有顯著性差異[第一天：P=0.111(16赫茲)和P=0.140(32赫茲)；第三十天：P=0.313(16赫茲)和P=0.344(32赫茲)]。前庭功能檢查：對照組：頭偏實驗中,慶大霉素注射結束后第一天3個大鼠出現(xiàn)嚴重頭偏(40℃)；4個出現(xiàn)中度頭偏(10-40℃)；實驗組中未出現(xiàn)(P=0.010),統(tǒng)計學有顯著性差異。提尾實驗中,對照組：4個大鼠出現(xiàn)不能控制的頭旋轉；實驗組中未出現(xiàn)(P=0.039),統(tǒng)計學上有顯著性差異。游泳實驗中對照組中5個大鼠出現(xiàn)游泳時間延長；實驗組中1個大鼠出現(xiàn)游泳時間延長(P=0.135)。形態(tài)學檢查：耳蝸,前庭毛細胞掃描電鏡和共聚焦顯微鏡檢查結果和功能檢查結果一致,統(tǒng)計學上耳蝸毛細胞和球囊斑/橢圓囊斑毛細胞計數(shù)均有顯著差異(P0.01)。 結論：口服高麗紅參對單側鼓膜內注射慶大霉素引起的耳前庭功能損傷有顯著的保護作用,對聽力損傷有保護作用,但功能評價上統(tǒng)計學意義不顯著,形態(tài)學評價上有顯著保護作用。
[Abstract]:Aim: to study the protective effect of Rhizoma Koryo Red Ginseng on auditory vestibular function and hearing impairment induced by gentamicin injection into the tympanic membrane of sD rats. Methods: 22 7-week-old sD rats were divided into control group and experimental group. Control group (n = 12): gentamicin was injected into the tympanic membrane of left ear (dose: 20ul, concentration: 500mg / ml, twice a day, seven times). Experimental group: 7 days before the injection of gentamicin, gavage of Koryo Red Ginseng powder solution (2 ml / 500 mg / kg, once a day), the seventh time of Gentamycin injection, the same as the control group. The vestibular function and hearing function were examined before gentamicin injection. The methods of vestibular function examination included head deviation test, lift and tail test, swimming test and animal treadmill test. Hearing tests include auditory brainstem response tests. Vestibular function and hearing function were examined on day 1, day 8, day 15, day 22 and day 30 after the experiment. The vestibular organ of rat cochlea was fixed at the end of the 30th day. The hair cells of cochlea follicle and oval follicle were observed by scanning electron microscope and confocal microscope. Results Statistical analysis was carried out. Results: (1) functional examination: vestibular function and hearing function were normal before experiment. On the first day after the trial, the hearing threshold was increased to 58.3 鹵9.9dB (16 Hz) and 57.1 鹵8.7dB (32 Hz), and on the 30th day to 45.8 鹵9.5dB (16 Hz) and 42.1 鹵9.1dB (32 Hz). In the experimental group, the hearing threshold was increased to 51.0 鹵10.8dB (16 Hz) and 51.0 鹵9.9dB (32 Hz), and on the 30th day to 41.0 鹵12.4dB (16 Hz) and 37.5 鹵12.9 dB (32 Hz). Results all the experimental groups were lower than those of the control group, but there was no significant difference in the statistical results [first day: P0. 111 (16 Hz) and P0. 140 (32 hertz); 30 days: P0. 313 (16 Hz) and P0. 344 (32 Hz)]. Vestibular function examination: in the control group, three rats showed severe head deviation on the first day after gentamicin injection (40 鈩，

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