發(fā)布時間：2018-10-12 08:51

【摘要】： 隨著對喉癌研究的深入,逐漸認(rèn)識到淋巴結(jié)轉(zhuǎn)移和腫瘤局部復(fù)發(fā)是影響喉癌預(yù)后的主要因素。如何提高喉癌轉(zhuǎn)移的早期診斷水平是及時正確處理頸部轉(zhuǎn)移病灶的關(guān)鍵,有益于提高臨床生存率。 轉(zhuǎn)錄抑制因子Snail和上皮鈣黏蛋白E-cadherin在上皮-間質(zhì)轉(zhuǎn)化(epithelial-esenchymal transition,EMT)過程中發(fā)揮著重要作用。Snail通過與E-cadherin啟動子區(qū)的E-盒(E-box)結(jié)合,降低E-cadherin的表達(dá)進而誘導(dǎo)EMT的發(fā)生,從而促進腫瘤細(xì)胞從原發(fā)位置脫落,進而發(fā)生轉(zhuǎn)移。國內(nèi)外關(guān)于Snail和E-cadherin在腫瘤轉(zhuǎn)移方面的研究,多致力于卵巢癌、結(jié)腸癌、食道癌等上皮性腫瘤的研究,尚未見兩者在喉鱗癌方面的系統(tǒng)研究。本研究通過闡明Snail、E-cadherin在喉鱗癌、癌旁組織及息肉中的表達(dá),來分析兩者與喉鱗癌發(fā)生、發(fā)展的關(guān)系,并探討Snail、E-cadherin在喉鱗癌中的表達(dá)與喉鱗癌患者臨床病理各因素的關(guān)系,以及Snail、E-cadherin在喉鱗狀細(xì)胞癌中表達(dá)的相關(guān)性。本實驗通過免疫組織化學(xué)染色(S-P法)發(fā)現(xiàn):Snail在喉鱗癌中的表達(dá)明顯高于癌旁組織、聲帶息肉組織,而E-cadherin蛋白的表達(dá)與其相反;Snail的高表達(dá)與喉鱗癌的組織分化程度、頸淋巴結(jié)轉(zhuǎn)移有關(guān),E-cadherin表達(dá)的降低與喉鱗狀細(xì)胞癌的組織分化程度、T分級、臨床分期以及頸淋巴結(jié)轉(zhuǎn)移有關(guān);在喉鱗癌中Snail和E-cadherin的表達(dá)呈負(fù)相關(guān),上述結(jié)果說明兩者聯(lián)合檢測可作為喉鱗癌惡性程度和預(yù)后的參考指標(biāo),并可為個性化治療提供參考信息。
[Abstract]:With the further study of laryngeal carcinoma, it is gradually recognized that lymph node metastasis and local recurrence are the main factors affecting the prognosis of laryngeal carcinoma. How to improve the level of early diagnosis of laryngeal cancer metastasis is the key to correct treatment of cervical metastases in time, which is beneficial to improve the clinical survival rate. Transcription suppressor Snail and E-cadherin play an important role in the process of epithelial-interstitial transformation (epithelial-esenchymal transition,EMT). Snail reduces the expression of E-cadherin and induces EMT by binding to the E-box of E-cadherin promoter. Thus promoting tumor cells from the primary location of the exfoliation, and then metastasis. At home and abroad, Snail and E-cadherin in tumor metastasis, mostly devoted to ovarian cancer, colon cancer, esophageal cancer and other epithelial tumors, there is no systematic study of the two in laryngeal squamous cell carcinoma. In this study, the expression of Snail,E-cadherin in laryngeal squamous cell carcinoma (LSCC), paracancerous tissues and polyps was studied to analyze the relationship between the expression of Snail,E-cadherin and the occurrence and development of laryngeal squamous cell carcinoma (LSCC), and to explore the relationship between the expression of Snail,E-cadherin in laryngeal squamous cell carcinoma (LSCC) and the clinicopathological factors of LSCC. And the correlation of Snail,E-cadherin expression in laryngeal squamous cell carcinoma. The expression of Snail in laryngeal squamous cell carcinoma (LSCC) was significantly higher than that in paracancerous tissues and vocal cord polyps, while the expression of E-cadherin protein was contrary to S-P method, and the high expression of Snail was associated with the differentiation of laryngeal squamous cell carcinoma (LSCC). The decrease of E-cadherin expression was related to the histological differentiation, T grade, clinical stage and cervical lymph node metastasis of laryngeal squamous cell carcinoma, and the expression of Snail and E-cadherin was negatively correlated in laryngeal squamous cell carcinoma. These results suggest that the combined detection can be used as a reference index for the malignancy and prognosis of laryngeal squamous cell carcinoma and can provide reference information for individualized treatment.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.65

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本文編號：2265520