【摘要】：目的:探討伴有kazal域的富含半胱氨酸的逆轉(zhuǎn)誘導(dǎo)蛋白(revemion-inducing-cysteine- rich protein with kazal motifs, RECK)和基質(zhì)金屬蛋白酶-9(matrix metalloproteinase-9 MMP-9)在喉鱗狀細(xì)胞癌和癌旁不同距離組織中的表達(dá)及其與臨床的關(guān)系。 方法:采用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(reverse transcriptase polymerase chain reaction,RT-PCR)法及免疫組化SP法檢測(cè)38例喉鱗狀細(xì)胞癌及其癌旁0.2cm、0.5cm、1.0cm組織中RECK和MMP-9的表達(dá)情況,并結(jié)合臨床,分析其臨床病理參數(shù)的關(guān)系。 結(jié)果:RECK在喉鱗癌組織、癌旁0.2cm、0.5cm及1.0cm的組織中表達(dá)強(qiáng)度及表達(dá)率依次增高,組間比較有顯著統(tǒng)計(jì)學(xué)差異(P0.001);MMP-9在喉鱗癌組織、癌旁0.2cm、0.5cm及1.0cm的表達(dá)強(qiáng)度及表達(dá)率依次降低,組間比較有顯著統(tǒng)計(jì)學(xué)差異(P0.001)。RECK和MMP-9在癌組織分別與癌旁0.5cm和1.0cm的組織比較及在癌旁0.2cm分別與癌旁0.5cm和1.0cm的組織比較,表達(dá)均有統(tǒng)計(jì)學(xué)差異(P0.05);但癌組織與癌旁0.2cm的組織中比較及癌旁0.5cm與癌旁1.0cm的組織中比較,表達(dá)均無統(tǒng)計(jì)學(xué)差異(P0.05)。RECK和MMP-9的表達(dá)呈負(fù)相關(guān)(P0.05)。喉鱗癌中不同臨床分期、病理組織學(xué)分級(jí),淋巴轉(zhuǎn)移情況,二者的表達(dá)有統(tǒng)計(jì)學(xué)差異(P0.05)。 結(jié)論:RECK在喉鱗癌組織中呈陰性表達(dá),而MMP-9在喉鱗癌組織中則為陽性表達(dá);二者的表達(dá)呈負(fù)相關(guān);RECK和MMP-9與喉鱗癌的臨床分期、分化程度和淋巴轉(zhuǎn)移情況有相關(guān)性;二指標(biāo)的表達(dá)在癌旁0.5 cm處有明顯的變化。
[Abstract]:Aim: to investigate the expression of cysteine-rich reverse inducible protein (revemion-inducing-cysteine- rich protein with kazal motifs, RECK) and matrix metalloproteinase-9 (matrix metalloproteinase-9 MMP-9) with kazal domain in laryngeal squamous cell carcinoma (LSCC) and adjacent tissues. Methods: reverse transcriptase polymerase chain reaction (reverse transcriptase polymerase chain reaction,RT-PCR) and immunohistochemistry (SP) were used to detect the expression of RECK and MMP-9 in 38 cases of laryngeal squamous cell carcinoma and its adjacent 0. 2 cm + 0. 5 cm ~ (-1). The relationship between the clinical pathological parameters and the clinical pathological parameters was analyzed. Results the expression intensity and expression rate of 1.0cm in laryngeal squamous cell carcinoma (LSCC) tissues were significantly higher than those in normal laryngeal squamous cell carcinoma tissues (P0.001). The expression intensity and expression rate of MMP-9 in laryngeal squamous cell carcinoma tissues were significantly lower than those in normal laryngeal squamous cell carcinoma tissues (P0.001). The expression intensity and expression rate of MMP 9 and 1.0cm in the adjacent tissues of laryngeal squamous cell carcinoma (LSCC) were lower than those in normal laryngeal squamous cell carcinoma (P0.001). There were significant differences between the two groups (P0.001) .Reck and MMP-9 were compared with 0.5cm and 1.0cm in cancer tissue and 0.5cm and 1.0cm in adjacent 0.2cm, respectively. But there was no significant difference in the expression of 0.5cm and 1.0cm between cancer tissues and adjacent 0.2cm tissues (P0.05). The expression of reck and MMP-9 were negatively correlated (P0.05). In laryngeal squamous cell carcinoma, different clinical stages, histopathological grading, lymphatic metastasis, the expression of statistical difference between them (P0.05). Conclusion the expression of fraction reck is negative in laryngeal squamous cell carcinoma and MMP-9 is positive in laryngeal squamous cell carcinoma, and the expression of reck and MMP-9 are negatively correlated with the clinical stage, differentiation and lymphatic metastasis of laryngeal squamous cell carcinoma. The expression of two markers was significantly changed at 0. 5 cm adjacent to cancer.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R739.65

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