【摘要】：目的:通過采用免疫組織化學(xué)SP法,觀察組織蛋白酶B (cathepsinB, CB)在剝脫綜合征性白內(nèi)障以及單純老年性白內(nèi)障的晶狀體上皮細(xì)胞(1ens epithelial cell,LEC)中表達(dá),探討組織蛋白酶B與剝脫綜合征及其并發(fā)癥的相關(guān)性。 方法:應(yīng)用鏈霉菌親生物素蛋白-過氧化物酶標(biāo)免疫組織化學(xué)SP法染色,檢測組織蛋白酶B在剝脫綜合征性白內(nèi)障(A組15例)及單純老年性白內(nèi)障(B組15例)晶狀體上皮細(xì)胞中的表達(dá)情況(兩組均排除有眼部外傷史、腫瘤史及糖尿病史的患者)。 結(jié)果:組織蛋白酶B表達(dá)的陽性標(biāo)準(zhǔn)為晶狀體上皮細(xì)胞胞漿中呈黃色或棕黃色染色。組織蛋白酶B在剝脫綜合征性白內(nèi)障和單純老年性白內(nèi)障晶狀體上皮細(xì)胞中均有表達(dá),兩組的表達(dá)程度有很大差異。組織蛋白酶B在剝脫綜合征性白內(nèi)障晶狀體上皮細(xì)胞中的表達(dá):強(qiáng)陽性表達(dá)為7例、中等陽性表達(dá)為5例、弱陽性表達(dá)為2例、陰性表達(dá)為1例,該組表達(dá)得分為(?χ±s)為5.87士2.45;在單純老年性白內(nèi)障晶狀體上皮細(xì)胞中的陽性表達(dá):強(qiáng)陽性表達(dá)為1例、中等陽性表達(dá)為2例、弱陽性表達(dá)為7例、陰性表達(dá)為5例,該組表達(dá)得分為(?χ±s)為2.67士2.23。剝脫綜合征性白內(nèi)障和單純老年性白內(nèi)障晶狀體上皮細(xì)胞中的組織蛋白酶B表達(dá)采用wilcoxon秩和檢驗,P=0.0020.01,兩組之間差異有顯著統(tǒng)計學(xué)意義,兩組的表達(dá)得分(?χ±s)采用t檢驗,P=0.0010.01,兩組之間差異有顯著統(tǒng)計學(xué)意義。 結(jié)論:組織蛋白酶B與剝脫綜合征性白內(nèi)障的發(fā)生、發(fā)展有關(guān),可能與剝脫綜合征中剝脫物的來源有關(guān)。
[Abstract]:Objective: to observe the expression of cathepsin B (cathepsinB, CB) in lens epithelial cells (1ens epithelial cell,LEC) of patients with exfoliative syndromic cataract and simple senile cataract by immunohistochemical SP method. To investigate the relationship between cathepsin B and exfoliation syndrome and its complications. Methods: streptomyces biotin-peroxidase immunohistochemical SP staining was used. The expression of cathepsin B in lens epithelial cells of exfoliative cataract (group A, n = 15) and senile cataract (group B, n = 15) was examined. Results: the positive standard of cathepsin B expression was yellow or brown staining in the cytoplasm of lens epithelial cells. Cathepsin B was expressed in lens epithelial cells of both exfoliative syndromic cataract and simple senile cataract. The degree of expression of cathepsin B was significantly different between the two groups. The expression of cathepsin B in lens epithelial cells of cataract with exfoliation syndrome: 7 cases had strong positive expression, 5 cases had moderate positive expression, 2 cases had weak positive expression, and 1 case had negative expression. The expression score of this group was 5.87 鹵2.45.The positive expression in lens epithelial cells of simple senile cataract was 1 case of strong positive expression, 2 cases of moderate positive expression, 7 cases of weak positive expression and 5 cases of negative expression. The score of expression in this group was 2.67 鹵2.23 (蠂 ~ 2 鹵s). The expression of cathepsin B in lens epithelial cells of exfoliative syndromic cataract and simple senile cataract was detected by wilcoxon rank sum test (P = 0.0020.01). The difference between the two groups was statistically significant. The expression score (蠂 ~ 2 鹵s) of the two groups was measured by t test (P = 0.0010.01), and there was significant difference between the two groups. Conclusion: cathepsin B is related to the occurrence and development of peeling syndrome cataract, and may be related to the origin of stripping substance in stripping syndrome.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R776.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 翁景寧,張惠蓉;PCNA反義寡脫氧核苷酸對牛晶狀體上皮細(xì)胞體外增殖活性的影響[J];眼科研究;2001年03期

2 劉寶海,譚少健,韋敏怡,梁皓,李霞,蔣林志;γ干擾素對兔晶狀體上皮細(xì)胞增殖細(xì)胞核抗原表達(dá)的影響[J];眼科;2004年05期

3 譚健,姚克,王凱軍,徐雯,申屠形超;環(huán)孢素A對兔晶狀體上皮細(xì)胞增殖的影響[J];眼科研究;2005年02期

4 譚淺,羅棟強(qiáng),蔣劍;骨橋蛋白在白內(nèi)障晶狀體上皮細(xì)胞中的表達(dá)[J];國際眼科雜志;2005年04期

5 張雪巖,張勁松,孔瑋;人晶狀體上皮細(xì)胞內(nèi)熱休克蛋白mRNA的表達(dá)[J];眼科新進(jìn)展;2005年05期

6 崔t熈，

本文編號：2213800