c-FLIP和RECK在喉鱗癌及癌旁組織中的表達(dá)及對(duì)于喉癌手術(shù)的指導(dǎo)意義
發(fā)布時(shí)間：2018-08-28 07:32
【摘要】： 目的：1,探討癌基因c-FLIP和抑癌基因RECK在喉癌組織中的表達(dá)與喉癌臨床特征的關(guān)系,了解喉癌的生物學(xué)行為。并從細(xì)胞和基因水平探討c-FLIP和RECK在喉癌及癌旁不同距離粘膜組織中表達(dá)變化特征,分析兩者作為喉癌特異性分子指標(biāo)的可行性,并探討喉癌手術(shù)粘膜安全切緣的范圍。2,研究RECK因子在聲門上喉癌中的表達(dá)特征,初步探索聲門上喉癌粘膜下浸潤的距離及深部切緣安全界的標(biāo)準(zhǔn)。 方法：1,分別采用PV9000免疫組織化學(xué)兩步法及RT-PCR方法檢測38例喉磷癌組織及距癌組織邊緣2mm、5mm、10mm粘膜組織中c-FLIP和RECK蛋白的表達(dá)及mRNA轉(zhuǎn)錄情況,同時(shí)取距離癌組織邊緣15mm處粘膜組織作為對(duì)照。2,應(yīng)用免疫組化方法檢測24例聲門上喉鱗癌及粘膜下浸潤不同距離的深部切緣組織中RECK蛋白的表達(dá),并采用圖像分析技術(shù),計(jì)算蛋白表達(dá)的平均光密度值,結(jié)果采用均數(shù)±標(biāo)準(zhǔn)差(mean±SD)的形式表示,并進(jìn)行方差分析,組間采用SNK檢驗(yàn)。 結(jié)果：1, c-FLIP和RECK在喉鱗癌組織中的表達(dá)水平與患者臨床分期和腫瘤病理學(xué)分級(jí)分別成正相關(guān)和負(fù)相關(guān)。c-FLIP在伴頸淋巴結(jié)轉(zhuǎn)移組中的表達(dá)水平明顯高于非轉(zhuǎn)移組,而RECK則正好相反。2,c-FLIP表達(dá)情況依癌組織→癌旁2mm→癌旁5mm→癌旁10mm→癌旁15mm順序遞減,而RECK則遞增。c-FLIP在癌組織與癌旁5mm、10mm、15mm組織之間表達(dá)水平有顯著性差異(P0.05),而與癌旁2mm處組織之間差別無統(tǒng)計(jì)學(xué)意義。RECK在癌組織與癌旁5mm、10mm、15mm組織之間表達(dá)水平有顯著性差異(P0.05),而與癌旁2mm處組織之間差別無統(tǒng)計(jì)學(xué)意義。3,RECK蛋白在聲門上喉癌中的表達(dá)與病理分化程度及臨床分期有密切關(guān)系。4,RECK蛋白的表達(dá)量在聲門上喉癌及不同距離深部切緣組織中沿癌組織→2mm→5mm→10mm→15mm這樣的順序呈現(xiàn)出遞增趨勢(shì)。平均光密度值測定得出癌組織,2mm,5mm處組織三者之間表達(dá)無明顯差別(P=0.088),10mm與15mm處組織表達(dá)也無差別(P=0.251),但前三者與后兩者之間的表達(dá)量有顯著差別(P0.01)。 結(jié)論：1,c-FLIP和RECK兩因子在喉鱗癌組織中的表達(dá)水平與喉鱗癌患者的臨床病理參數(shù)密切相關(guān)。2,c-FLIP和RECK可能都參與了喉鱗癌的發(fā)生、發(fā)展,并與喉鱗癌的浸潤及轉(zhuǎn)移有密切關(guān)系。3,c-FLIP和RECK在喉鱗癌組織中及癌旁各距離粘膜組織中表達(dá)情況進(jìn)一步表明以距腫瘤邊緣5mm作為粘膜安全切緣的標(biāo)準(zhǔn)較為適宜。4,聲門上喉癌粘膜下深部切緣的安全切除范圍可能達(dá)到0.5.1cm之間,有待于術(shù)后隨訪進(jìn)一步證實(shí)。
[Abstract]:Objective to investigate the relationship between the expression of oncogene c-FLIP and tumor suppressor gene RECK and the clinical features of laryngeal carcinoma, and to understand the biological behavior of laryngeal carcinoma. The expression characteristics of c-FLIP and RECK in laryngeal carcinoma and adjacent mucosa tissues were studied from cell and gene levels, and the feasibility of using them as specific molecular markers of laryngeal carcinoma was analyzed. To study the expression of RECK factor in supraglottic laryngeal carcinoma, and to explore the distance of submucosal invasion of supraglottic laryngeal carcinoma and the standard of deep margin safety. Methods the expression of c-FLIP and RECK protein and the transcription of mRNA in 38 cases of laryngeal phosphorous carcinoma and 2mm ~ 5mm ~ 10mm from the margin of laryngeal carcinoma were detected by PV9000 immunohistochemical two-step method and RT-PCR method respectively. The expression of RECK protein in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration was detected by immunohistochemical method, and the expression of RECK protein was detected by image analysis technique in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration. The average optical density of protein expression was calculated. The results were expressed in the form of mean 鹵standard deviation (mean 鹵SD), analysis of variance and SNK test between groups. Results the expression levels of c-FLIP and RECK in laryngeal squamous cell carcinoma were positively and negatively correlated with clinical stage and tumor pathological grade, respectively. The expression of c-FLIP in patients with cervical lymph node metastasis was significantly higher than that in non-metastasis group. On the contrary, the expression of c-FLIP in RECK decreased according to the sequence of 15mm in adjacent 2mm and 5mm adjacent to 5mm. However, the expression of c-FLIP in cancer tissue was significantly higher than that in adjacent 2mm tissue (P0.05), but there was no significant difference between c-FLIP and adjacent 2mm tissue (P0.05). There was a significant difference in the expression level of reck between cancer tissue and adjacent 5mm ~ 10mm ~ 15mm tissue (P0.05). (P0.05), but there was no significant difference between the expression of Reck protein and adjacent 2mm tissues. 3The expression of Reck protein in supraglottic laryngeal carcinoma was closely related to the degree of pathological differentiation and clinical stage. The expression of Reck protein in supraglottic laryngeal carcinoma and deep resection at different distances was closely related to the degree of pathological differentiation and clinical stage. The sequence of 2mm 5mm 10mm 15mm in marginal tissue showed an increasing trend. The average optical density value showed that there was no significant difference between the three groups (P0. 088) and 15mm (P0. 251), but there was a significant difference between the first three and the latter (P0. 01). Conclusion the expression level of c-FLIP and RECK in laryngeal squamous cell carcinoma is closely related to the clinicopathological parameters of laryngeal squamous cell carcinoma. Both c-FLIP and RECK may be involved in the occurrence and development of laryngeal squamous cell carcinoma. The expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosal tissues further indicated that 5mm from the edge of the tumor as the safe margin of mucous membrane is more suitable for .4. the expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosa tissues is more suitable. The safe resection range of the deep incisal margin of suprahilar laryngocarcinoma may be between 0.5.1cm, To be further confirmed by postoperative follow-up.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R739.65
本文編號(hào)：2208714
[Abstract]:Objective to investigate the relationship between the expression of oncogene c-FLIP and tumor suppressor gene RECK and the clinical features of laryngeal carcinoma, and to understand the biological behavior of laryngeal carcinoma. The expression characteristics of c-FLIP and RECK in laryngeal carcinoma and adjacent mucosa tissues were studied from cell and gene levels, and the feasibility of using them as specific molecular markers of laryngeal carcinoma was analyzed. To study the expression of RECK factor in supraglottic laryngeal carcinoma, and to explore the distance of submucosal invasion of supraglottic laryngeal carcinoma and the standard of deep margin safety. Methods the expression of c-FLIP and RECK protein and the transcription of mRNA in 38 cases of laryngeal phosphorous carcinoma and 2mm ~ 5mm ~ 10mm from the margin of laryngeal carcinoma were detected by PV9000 immunohistochemical two-step method and RT-PCR method respectively. The expression of RECK protein in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration was detected by immunohistochemical method, and the expression of RECK protein was detected by image analysis technique in 24 cases of supraglottic laryngeal squamous cell carcinoma (SCSCC) and deep incised margin tissues with submucosal infiltration. The average optical density of protein expression was calculated. The results were expressed in the form of mean 鹵standard deviation (mean 鹵SD), analysis of variance and SNK test between groups. Results the expression levels of c-FLIP and RECK in laryngeal squamous cell carcinoma were positively and negatively correlated with clinical stage and tumor pathological grade, respectively. The expression of c-FLIP in patients with cervical lymph node metastasis was significantly higher than that in non-metastasis group. On the contrary, the expression of c-FLIP in RECK decreased according to the sequence of 15mm in adjacent 2mm and 5mm adjacent to 5mm. However, the expression of c-FLIP in cancer tissue was significantly higher than that in adjacent 2mm tissue (P0.05), but there was no significant difference between c-FLIP and adjacent 2mm tissue (P0.05). There was a significant difference in the expression level of reck between cancer tissue and adjacent 5mm ~ 10mm ~ 15mm tissue (P0.05). (P0.05), but there was no significant difference between the expression of Reck protein and adjacent 2mm tissues. 3The expression of Reck protein in supraglottic laryngeal carcinoma was closely related to the degree of pathological differentiation and clinical stage. The expression of Reck protein in supraglottic laryngeal carcinoma and deep resection at different distances was closely related to the degree of pathological differentiation and clinical stage. The sequence of 2mm 5mm 10mm 15mm in marginal tissue showed an increasing trend. The average optical density value showed that there was no significant difference between the three groups (P0. 088) and 15mm (P0. 251), but there was a significant difference between the first three and the latter (P0. 01). Conclusion the expression level of c-FLIP and RECK in laryngeal squamous cell carcinoma is closely related to the clinicopathological parameters of laryngeal squamous cell carcinoma. Both c-FLIP and RECK may be involved in the occurrence and development of laryngeal squamous cell carcinoma. The expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosal tissues further indicated that 5mm from the edge of the tumor as the safe margin of mucous membrane is more suitable for .4. the expression of c-FLIP and RECK in laryngeal squamous cell carcinoma tissues and adjacent mucosa tissues is more suitable. The safe resection range of the deep incisal margin of suprahilar laryngocarcinoma may be between 0.5.1cm, To be further confirmed by postoperative follow-up.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R739.65
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