不同基質(zhì)眼膏進(jìn)入豚鼠眼眶的不良反應(yīng)
發(fā)布時(shí)間：2018-08-25 07:50
【摘要】：眼膏是眼科藥物的常用劑型,具有在結(jié)膜囊內(nèi)滯留時(shí)間長(zhǎng),能減輕眼瞼對(duì)眼球摩擦等優(yōu)點(diǎn),但通過(guò)手術(shù)創(chuàng)口、淚道探通創(chuàng)口或者假道進(jìn)入眼瞼皮下、結(jié)膜下或眼眶軟組織內(nèi),可引起嚴(yán)重的組織反應(yīng)。臨床上遇到多起淚道探通或淚道激光疏通治療后,注入的眼膏進(jìn)入眼瞼或眼眶組織中,以及耳鼻喉科手術(shù)后填塞凡士林紗條,引起嚴(yán)重眶內(nèi)組織反應(yīng)的病例。 本實(shí)驗(yàn)是將以凡士林、羊毛脂和液體石蠟為基質(zhì)的紅霉素眼膏和以玻璃酸鈉和卡波姆為基質(zhì)的氧氟沙星眼膏以及羥丙基甲基纖維素分別注入眼眶,建立不同基質(zhì)眼膏進(jìn)入眼眶軟組織的動(dòng)物模型,觀察和比較進(jìn)入眼眶軟組織的不良反應(yīng)。 材料與方法 動(dòng)物與分組健康清潔級(jí)豚鼠63只(鄭州大學(xué)實(shí)驗(yàn)動(dòng)物中心提供),雌雄不限,年齡1-2周,體重100g-140 g,排除各種眼科疾患。以眼周淺色為宜,便于大體觀察。63只豚鼠隨機(jī)分為A、B、C組,每組21組。 動(dòng)物模型制作消毒眼瞼周圍皮膚,使用10%的水合氯醛0.3g/k腹腔內(nèi)注射誘導(dǎo)全麻。用1 ml注射器22 G針頭從眶下緣的外1/3與內(nèi)2/3交界處皮膚進(jìn)針達(dá)眼球后,注入以凡士林、液體石蠟和羊毛脂為紅霉素眼膏(A組)、以玻璃酸鈉和卡波姆為基質(zhì)的氧氟沙星眼膏(B組)、羥丙基甲基纖維素(C組)各0.3ml,正常對(duì)照眼不做處理。 觀察項(xiàng)目和時(shí)間點(diǎn)分別在注射后2 h、1 d、3 d、7 d、14 d、21d、28 d七個(gè)時(shí)間點(diǎn)進(jìn)行觀察。記錄眼部紅腫、腫脹情況、眼球突出情況、球后阻力等炎癥反應(yīng)情況。每時(shí)間點(diǎn)每組隨機(jī)處死3只豚鼠,觀察大體標(biāo)本上眼膏在眶內(nèi)的擴(kuò)散情況、HE染色組織病理學(xué)變化。 統(tǒng)計(jì)學(xué)分析處理應(yīng)用SPSS17.0統(tǒng)計(jì)軟件包,3組間炎癥反應(yīng)的有序變量資料比較采用秩和檢驗(yàn),取α=0.05為檢驗(yàn)水準(zhǔn)。 結(jié)果 A組早期結(jié)膜腫脹、眼球突出,球后阻力增加最為嚴(yán)重,持續(xù)時(shí)間最長(zhǎng),病理早期以中性粒細(xì)胞為主的炎癥細(xì)胞浸潤(rùn)及晚期可見成纖維細(xì)胞增生包裹,晚期可見成纖維細(xì)胞增生,異物肉芽腫形成,炎癥浸潤(rùn)和細(xì)胞增殖反應(yīng)最重。 B組早期炎癥反應(yīng)重,以中性粒細(xì)胞和嗜酸性粒細(xì)胞為主的炎癥細(xì)胞浸潤(rùn),且炎細(xì)胞數(shù)目明顯多于A組,但未見成纖維細(xì)胞增生。 C組注射后眶組織充血水腫消失快,炎癥細(xì)胞最少,未見纖維組織增生。 注射后3d各組間差異均有統(tǒng)計(jì)學(xué)意義(H=9.089,H=16.830,H=17.084,P均0.05)。 結(jié)論 以凡士林、羊毛脂和液狀石蠟為基質(zhì)的眼膏進(jìn)入豚鼠眶軟組織后,引起的組織炎癥反應(yīng)最重,局部纖維組織增生,形成異物肉芽腫,降解所需時(shí)間較長(zhǎng)。 以玻璃酸鈉和卡波姆為基質(zhì)的眼膏進(jìn)豚鼠眶軟組織后,3d內(nèi)可降解、吸收,局部無(wú)纖維組織增生。 羥丙基甲基纖維素引起的局部炎癥反應(yīng)最輕,24 h內(nèi)可完全降解、吸收,無(wú)纖維組織增生。
[Abstract]:Eye ointment is a commonly used dosage form of ophthalmic drugs. It has the advantages of long stay time in conjunctival sac and can reduce the eyelid to eyeball friction. But through the operation wound, lacrimal duct probing the wound or false passage into the eyelid, subconjunctival or orbital soft tissue, etc. Can cause severe tissue reactions. There were many cases of lacrimal duct exploration or lacrimal duct laser therapy, the injected ointment entered the eyelid or orbital tissue, and the vaseline gauze was filled after the operation of otolaryngology, which caused serious intraorbital tissue reaction. In this experiment, erythromycin eye ointment based on vaseline, lanolin and liquid paraffin, ofloxacin eye ointment based on sodium hyaluronate and carbomer and hydroxypropyl methyl cellulose were injected into orbit, respectively. The animal model of different matrix ointment entering orbital soft tissue was established to observe and compare the adverse reaction of entering orbital soft tissue. Materials and methods Sixty-three healthy clean grade guinea pigs (provided by Experimental Animal Center, Zhengzhou University), aged 1-2 weeks, weight 100g-140 g, were excluded from all kinds of ophthalmic diseases. 63 guinea pigs were randomly divided into two groups: group A (n = 21) and group C (n = 21). The skin around eyelid was disinfected with 10% chloral hydrate 0.3g/k intraperitoneally to induce general anesthesia. A 22G needle was injected into the eyeball with a 22 G needle from the outer third of the inferior orbital margin to the inner 2 / 3 of the eye, and vaseline was injected into the eyeball. Liquid paraffin and lanolin were erythromycin eye ointment (group A), ofloxacin eye ointment (group B) with sodium hyaluronate and carbomer as matrix, hydroxypropyl methyl cellulose (group C) as 0.3 ml respectively. The observation items and time points were observed at 2 h, 1 d, 3 d, 7 d, 14 d, 21 d and 28 d, respectively. Record inflammatory reactions such as swelling, exophthalmos, retrobulbar resistance and so on. At each time point, 3 guinea pigs were randomly killed in each group to observe the diffusion of the ointment in the orbit and the histopathological changes of HE staining. SPSS17.0 statistical software package was used to analyze the order variables of inflammatory reaction in the three groups. The rank sum test was used to compare the data, and 偽 -0. 05 was taken as the test level. Results in group A, the early conjunctiva swelling, exophthalmos and retrobulbar resistance increased most seriously and lasted for the longest time. The infiltration of inflammatory cells with neutrophil in the early stage and fibroblast proliferation in the late stage were observed in group A. Fibroblast proliferation, granuloma formation, inflammatory infiltration and cell proliferation were observed in the late stage. In group B, the early inflammatory reaction was severe, and the inflammatory cell infiltration was dominated by neutrophil and eosinophilic granulocyte. The number of inflammatory cells was significantly more than that of group A, but no fibroblast proliferation was found in group C. in group C, hyperemia and edema disappeared quickly, inflammatory cells were the least, and fibrous tissue proliferation was not seen in group C. Three days after injection, there were significant differences among the three groups (P 0.05). Conclusion when the ointment with vaseline, lanolin and liquid paraffin as the base, the inflammation is the most serious, the local fibrous tissue proliferates, the foreign body granuloma is formed, and the degradation time is longer. The eye ointment based on sodium hyaluronate and carbomer could be degraded and absorbed in the orbital soft tissue of guinea pig within 3 days, and the local fibrous tissue could proliferate. The local inflammatory reaction induced by hydroxypropyl methylcellulose could be completely degraded, absorbed and no fibrous tissue proliferated within 24 h.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R77
本文編號(hào)：2202241
[Abstract]:Eye ointment is a commonly used dosage form of ophthalmic drugs. It has the advantages of long stay time in conjunctival sac and can reduce the eyelid to eyeball friction. But through the operation wound, lacrimal duct probing the wound or false passage into the eyelid, subconjunctival or orbital soft tissue, etc. Can cause severe tissue reactions. There were many cases of lacrimal duct exploration or lacrimal duct laser therapy, the injected ointment entered the eyelid or orbital tissue, and the vaseline gauze was filled after the operation of otolaryngology, which caused serious intraorbital tissue reaction. In this experiment, erythromycin eye ointment based on vaseline, lanolin and liquid paraffin, ofloxacin eye ointment based on sodium hyaluronate and carbomer and hydroxypropyl methyl cellulose were injected into orbit, respectively. The animal model of different matrix ointment entering orbital soft tissue was established to observe and compare the adverse reaction of entering orbital soft tissue. Materials and methods Sixty-three healthy clean grade guinea pigs (provided by Experimental Animal Center, Zhengzhou University), aged 1-2 weeks, weight 100g-140 g, were excluded from all kinds of ophthalmic diseases. 63 guinea pigs were randomly divided into two groups: group A (n = 21) and group C (n = 21). The skin around eyelid was disinfected with 10% chloral hydrate 0.3g/k intraperitoneally to induce general anesthesia. A 22G needle was injected into the eyeball with a 22 G needle from the outer third of the inferior orbital margin to the inner 2 / 3 of the eye, and vaseline was injected into the eyeball. Liquid paraffin and lanolin were erythromycin eye ointment (group A), ofloxacin eye ointment (group B) with sodium hyaluronate and carbomer as matrix, hydroxypropyl methyl cellulose (group C) as 0.3 ml respectively. The observation items and time points were observed at 2 h, 1 d, 3 d, 7 d, 14 d, 21 d and 28 d, respectively. Record inflammatory reactions such as swelling, exophthalmos, retrobulbar resistance and so on. At each time point, 3 guinea pigs were randomly killed in each group to observe the diffusion of the ointment in the orbit and the histopathological changes of HE staining. SPSS17.0 statistical software package was used to analyze the order variables of inflammatory reaction in the three groups. The rank sum test was used to compare the data, and 偽 -0. 05 was taken as the test level. Results in group A, the early conjunctiva swelling, exophthalmos and retrobulbar resistance increased most seriously and lasted for the longest time. The infiltration of inflammatory cells with neutrophil in the early stage and fibroblast proliferation in the late stage were observed in group A. Fibroblast proliferation, granuloma formation, inflammatory infiltration and cell proliferation were observed in the late stage. In group B, the early inflammatory reaction was severe, and the inflammatory cell infiltration was dominated by neutrophil and eosinophilic granulocyte. The number of inflammatory cells was significantly more than that of group A, but no fibroblast proliferation was found in group C. in group C, hyperemia and edema disappeared quickly, inflammatory cells were the least, and fibrous tissue proliferation was not seen in group C. Three days after injection, there were significant differences among the three groups (P 0.05). Conclusion when the ointment with vaseline, lanolin and liquid paraffin as the base, the inflammation is the most serious, the local fibrous tissue proliferates, the foreign body granuloma is formed, and the degradation time is longer. The eye ointment based on sodium hyaluronate and carbomer could be degraded and absorbed in the orbital soft tissue of guinea pig within 3 days, and the local fibrous tissue could proliferate. The local inflammatory reaction induced by hydroxypropyl methylcellulose could be completely degraded, absorbed and no fibrous tissue proliferated within 24 h.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R77
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