【摘要】：目的:研究Clara細(xì)胞1O-KDa蛋白( Clara cell 1O-KDa protein,CC10)在小鼠變應(yīng)性鼻炎中的表達(dá),探討鼻腔滴入重組白介素10(interleukin-10,IL-10)對CC10表達(dá)調(diào)節(jié)。 方法:在第0、5天,用卵清蛋白(ovalbumin,OVA)對C57BL/6J小鼠進(jìn)行腹腔注射來致敏,從第12至19天給予OVA行鼻腔激發(fā),每天一次。治療組分別用地塞米松(dexamethasone,DXM)和重組IL-10于鼻腔激發(fā)前行腹腔注射和鼻腔滴入,對照組用同樣方法致敏,但是激發(fā)用無菌生理鹽水代替OVA。分別用蘇木素-伊紅染色、過碘酸-希夫染色、免疫組織化學(xué),檢測小鼠鼻粘膜組織浸潤的嗜酸性粒細(xì)胞、杯狀細(xì)胞和CC10表達(dá)。 結(jié)果:對照組CC10主要由鼻中隔上纖毛上皮細(xì)胞和鼻甲上小圓頂狀細(xì)胞分泌,而在OVA組表達(dá)顯著下降(p 0.05),經(jīng)過DXM或重組IL-10治療,CC10表達(dá)明顯上升,但是DXM作用要優(yōu)于重組IL-10。和對照組相比,嗜酸性粒細(xì)胞和杯狀細(xì)胞在DXM組和IL-10組增加,而纖毛上皮細(xì)胞和小圓頂狀細(xì)胞減少,而在OVA組這種改變更加顯著(p 0.05)。 結(jié)論:CC10可能作為一種內(nèi)源性因子參與了變應(yīng)性鼻炎的發(fā)病過程,并且能被外源性IL-10上調(diào),但是作用機(jī)制不清楚,需要進(jìn)一步研究。
[Abstract]:Aim: to study the expression of 1O-KDa protein (Clara cell 1O-KDa protein CC10 in Clara cells in mice with allergic rhinitis and to investigate the regulation of CC10 expression by nasal drip of recombinant interleukin-10 (IL-10). Methods: C57BL/6J mice were sensitized with ovalbumin OVA on day 0 to day 5. OVA was given nasal stimulation once a day from day 12 to day 19. In the treatment group, dexamethasone (DXM) and recombinant IL-10 were injected intraperitoneally and dripped into the nasal cavity before stimulation, while the control group was sensitized with the same method, but the aseptic saline was used instead of OVA. The expression of eosinophils goblet cells and CC10 in nasal mucosa of mice were detected by hematoxylin-eosin staining periodate-Schiff staining and immunohistochemistry. Results: in the control group, the expression of CC10 was mainly secreted by the epithelial cells of superior nasal septum ciliated and the small dome of the superior turbinate, but the expression of CC10 in the OVA group was significantly decreased (p0.05). The expression of CC10 was significantly increased after DXM or recombinant IL-10 treatment, but the effect of DXM was better than that of IL-10. Compared with the control group, eosinophil and goblet cells increased in DXM and IL-10 groups, but cilia epithelial cells and small domed cells decreased, but in OVA group the change was more significant (p0.05). Conclusion as an endogenous factor, the ratio CC10 may be involved in the pathogenesis of allergic rhinitis and can be up-regulated by exogenous IL-10, but the mechanism of action is not clear and needs further study.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R765.21

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