【摘要】：目的：通過對39例聲門上型喉癌及深部不同距離手術(shù)切緣CK-19表達(dá)的研究，為臨床確定不同分期聲門上型喉癌手術(shù)的深部安全切緣提供依據(jù)。 方法：收集臨床資料完整的聲門上型喉癌標(biāo)本39例，作縱向每3mm距離連續(xù)切片，取腫瘤最大面的組織塊連續(xù)切片，分別采用PV6001免疫組織化學(xué)兩步法及RT-PCR方法檢測39例聲門上型喉癌組織及不同距離深部切緣組織中CK-19的表達(dá)及CK-19mRNA轉(zhuǎn)錄情況。 結(jié)果：(1)TI-T2期聲門上型喉癌：CK-19表達(dá)癌組織與0.2cm、0.5cm、1cm深部切緣比較均有顯著性差異(p0.05)；0.2cm、0.5cm、1cm切緣三者之間無顯著性差異(p0.05)。(2)T3期聲門上型喉癌：CK-19表達(dá)癌組織與0.2cm切緣表達(dá)無顯著性差異(p0.05)；0.5cm和1cm無顯著性差異(p0.05)；癌組織與0.5cm，0.2cm與0.5cm有顯著性差異(p0.05)。(3)T4期聲門上型喉癌：CK-19表達(dá)癌組織與0.2cm、0.5cm表達(dá)均無顯著性差異(p0.05)；癌組織和1cm有顯著性差異(p0.05)；0.2cm與1cm、0.5cm與1cm之間均有顯著性差異(p0.05)。 結(jié)論：聲門上喉癌原發(fā)灶T分期越晚，腫瘤黏膜下侵襲程度越重。T1-T2期聲門上型喉癌，腫瘤深部2mm處CK-19表達(dá)與腫瘤組有明顯差異，手術(shù)切緣至少保證2mm以上；T3期聲門上型喉癌，腫瘤深部5mm處CK-19表達(dá)與腫瘤組差異明顯，手術(shù)切緣應(yīng)至少保證5mm；T4期聲門上型喉癌，腫瘤深部5mm處CK-19表達(dá)與腫瘤組無明顯差異，10mm處與腫瘤組有差異，手術(shù)切緣應(yīng)選擇5mm至10mm范圍。
[Abstract]:Objective: to study the expression of CK-19 in 39 patients with supraglottic laryngocarcinoma and deep resection margin in order to provide evidence for clinical determination of deep safe margin of supraglottic laryngeal carcinoma at different stages. Methods: 39 cases of supraglottic laryngeal carcinoma with complete clinical data were collected. The tissue mass of the largest surface of the tumor was sectioned continuously by longitudinal section per 3mm distance. The expression of CK-19 and CK-19mRNA transcription in 39 cases of supraglottic laryngeal carcinoma tissues and deep incised margin tissues were detected by PV6001 immunohistochemical two-step method and RT-PCR method respectively. Results: (1) there was no significant difference between the expression of CK-19 and the deep incisal margin of 0.2cm ~ (0.5) cm ~ (-1) in TI-T2 stage supraglottic carcinoma (p0.05). There was no significant difference among the three groups (p0.05). (_ 2). There was no significant difference between the expression of CK-19 and 0.2cm (p0.05). There was no significant difference between 0.5cm and 1cm (p0.05), and there was no significant difference between cancer tissue and 0.5 cm ~ 0.2cm and 0.5cm (p0.05). (_ 3). There was no significant difference in the expression of CK-19 and 0.2cm ~ (0.5 cm) between cancer tissues and 1cm (p0.05) in stage T4 supraglottic laryngocarcinoma (p0.05). There was a significant difference between 0.2cm and 1 cm, 0.5 cm and 1cm (p0.05). Conclusion: the later the T stage of supraglottic carcinoma is, the more severe the submucosal invasion is. T1-T2 stage supraglottic laryngeal carcinoma. The expression of CK-19 in the deep part of the tumor is significantly different from that in the tumor group, and the surgical margin at least ensures the supraglottic laryngeal carcinoma above 2mm stage T3. The expression of CK-19 in the deep 5mm of the tumor was significantly different from that in the tumor group. The operative margin should ensure at least 5mm T4 supraglottic laryngeal carcinoma. There was no significant difference in the expression of CK-19 between the deep 5mm of the tumor and the tumor group at 10mm and that in the tumor group. The range of 5mm to 10mm should be selected at the surgical margin.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R739.65

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 楊文鋒,王善政,左傳同;食管癌患者血清Cyfra21-1水平的研究[J];癌癥;2002年01期

2 李寶江,黃曉平,韋尉東,王軍業(yè),蘇曉東,張旭,洪文善,唐軍,張?zhí)m軍,龍浩,楊名添,戎鐵華;檢測乳腺癌患者骨髓中CK19的表達(dá)及其臨床意義[J];癌癥;2005年06期

3 梁建忠,黃國柱,謝裕達(dá),蘇毅;血清CEA、CA125、CYFRA21-1測定在肺癌診斷中的意義[J];第一軍醫(yī)大學(xué)學(xué)報(bào);2005年07期

4 黃志剛,韓德民;喉癌局部生長擴(kuò)散與喉內(nèi)“屏障作用”[J];耳鼻咽喉頭頸外科;2000年06期

5 閔鐘云,郭星,張爽,李舸;單層上皮細(xì)胞角蛋白在聲門上型喉癌中的表達(dá)[J];耳鼻咽喉頭頸外科;2003年06期

6 王斌全;溫樹信;皇甫輝;張海利;張春明;田俊;;喉癌的形態(tài)特征與黏膜下腫瘤侵襲的相關(guān)性[J];中國耳鼻咽喉頭頸外科;2008年07期

7 田俊;王斌全;皇甫輝;溫樹信;張海利;;細(xì)胞角蛋白抗體在喉鱗狀細(xì)胞癌淋巴結(jié)微轉(zhuǎn)移診斷中的應(yīng)用[J];中國耳鼻咽喉頭頸外科;2008年09期

8 馮燕;古亞蘭;聶敏海;張齊梅;梁尚爭;;細(xì)胞角蛋白19mRNA在口腔鱗狀細(xì)胞癌中表達(dá)的研究[J];華西口腔醫(yī)學(xué)雜志;2009年03期

9 羅榮,李貴澤,朱春生;喉癌患者血清細(xì)胞角蛋白片段21-1檢測及臨床意義[J];臨床耳鼻咽喉科雜志;2002年06期

10 侯麗;邵輝;高建中;;喉癌102例臨床分析[J];寧夏醫(yī)學(xué)雜志;2010年01期

，

本文編號(hào)：2178109