α-硫辛酸對(duì)大鼠視網(wǎng)膜缺血再灌注損傷中VEGF、MMP-9表達(dá)的影響
發(fā)布時(shí)間：2018-08-08 11:08
【摘要】： 目的探討α-硫辛酸對(duì)大鼠視網(wǎng)膜缺血再灌注損傷(retinal ischemia/reperfusion injury,RIRI)中血管內(nèi)皮生長(zhǎng)因子(vascular endothelial growth factor,VEGF)和基質(zhì)金屬蛋白酶-9(matrix metalloprotelnase-9,MMP-9)表達(dá)的影響及作用機(jī)制。 方法按隨機(jī)分組的原則將78只健康成年Wistar大鼠分為正常對(duì)照組6只,α-硫辛酸治療組(造模前3d開(kāi)始腹腔注射α-辛酸注射液100mg·kg-1,每天1次,直至處死)和缺血組(造模前3d開(kāi)始腹腔注射等量生理鹽水,每天1次,直至處死)各36只。采用生理鹽水前房灌注升高眼內(nèi)壓的方法建立視網(wǎng)膜缺血再灌注模型,維持眼內(nèi)壓110mmHg,持續(xù)60分鐘后拔出灌注針頭。分別于視網(wǎng)膜缺血再灌注后6h、12h、24h、48h、3d和7d處死大鼠,HE染色光鏡觀察視網(wǎng)膜組織變化,應(yīng)用免疫組織化學(xué)法檢測(cè)大鼠視網(wǎng)膜缺血再灌注損傷后視網(wǎng)膜組織VEGF和MMP-9表達(dá)的變化。 結(jié)果視網(wǎng)膜缺血再灌注后早期,視網(wǎng)膜內(nèi)層水腫增厚,晚期視網(wǎng)膜神經(jīng)節(jié)細(xì)胞數(shù)目減少及視神經(jīng)纖維層萎縮變薄。α-硫辛酸治療組組織變化趨勢(shì)與缺血組相似,細(xì)胞數(shù)目減少及視網(wǎng)膜厚度變薄的程度均較缺血組有明顯改善。正常對(duì)照組未觀察到明顯VEGF和MMP-9的表達(dá)(AOD分別為0.06±0.04和0.05±0.03)。缺血組和α-硫辛酸治療組在再灌注后12小時(shí)開(kāi)始出現(xiàn)VEGF的明顯表達(dá),并逐漸增加,到再灌注后48小時(shí)達(dá)到高峰(AOD分別為0.52±0.06和0.35±0.05), MMP-9的表達(dá)自再灌注后6小時(shí)開(kāi)始增加,24小時(shí)后達(dá)到高峰(AOD分別為0.38±0.06和0.29±0.05)。除了再灌注后6小時(shí)VEGF的表達(dá)外,其余各時(shí)間段缺血組VEGF、MMP-9的表達(dá)與正常對(duì)照組比較有統(tǒng)計(jì)學(xué)差異(P0.05), a-硫辛酸治療組與缺血再灌注組比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。經(jīng)相關(guān)性分析發(fā)現(xiàn),缺血組視網(wǎng)膜VEGF和MMP-9的表達(dá)呈正相關(guān)(r=0.834, P0.05)。 結(jié)論α-硫辛酸對(duì)RIRI有保護(hù)作用,可能通過(guò)抑制了RIRI中VEGF及MMP-9的表達(dá)而發(fā)揮作用。
[Abstract]:Objective to investigate the effect of 偽 -lipoic acid on the expression of vascular endothelial growth factor (vascular endothelial growth) and matrix metalloproteinase-9 (matrix metalloproteinase-9) in retinal ischemia/reperfusion injury-reperfusion injury in rats. Methods Seventy-eight healthy adult Wistar rats were randomly divided into normal control group (n = 6). 偽 -lipoic acid injection (100mg kg-1) was injected intraperitoneally 3 days before modeling. There were 36 rats in the ischemic group and 36 rats in the ischemic group (36 rats in each group were given intraperitoneal injection of the same amount of normal saline once a day until the death) 3 days before the model was made. The model of retinal ischemia-reperfusion was established by using normal saline anterior chamber perfusion to increase intraocular pressure. The intraocular pressure was maintained at 110 mm Hg.After 60 minutes, the perfusion needle was pulled out. The changes of retinal tissue were observed by HE staining at 6 h, 12 h, 24 h and 48 h, respectively, and the expression of VEGF and MMP-9 in retinal tissue of rats after retinal ischemia-reperfusion injury were detected by immunohistochemical method. Results in the early stage of retinal ischemia reperfusion, the thickness of retinal inner layer edema increased, the number of retinal ganglion cells decreased and the optic nerve fiber layer shrunk in late stage. The change trend of 偽 -lipoic acid treatment group was similar to that of ischemic group. The decrease in the number of cells and the thickness of retina were significantly improved compared with the ischemic group. No significant expression of VEGF and MMP-9 was observed in normal control group (AOD was 0.06 鹵0.04 and 0.05 鹵0.03, respectively). In ischemia group and 偽 -lipoic acid treatment group, the expression of VEGF began to appear 12 hours after reperfusion, and increased gradually. The expression of MMP-9 reached its peak at 48 hours after reperfusion (AOD was 0.52 鹵0.06 and 0.35 鹵0.05, respectively), and the expression of MMP-9 reached its peak at 24 hours after reperfusion (AOD was 0.38 鹵0.06 and 0.29 鹵0.05, respectively). In addition to the expression of VEGF at 6 hours after reperfusion, the expression of VEGF MMP-9 in ischemic group was significantly different from that in normal control group (P0.05), and there was a significant difference between a-lipoic acid treatment group and ischemia-reperfusion group (P0.05). The expression of VEGF and MMP-9 was positively correlated in ischemic group (r = 0.834, P 0.05). Conclusion 偽 -lipoic acid can protect RIRI by inhibiting the expression of VEGF and MMP-9 in RIRI.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R774.1
本文編號(hào)：2171602
[Abstract]:Objective to investigate the effect of 偽 -lipoic acid on the expression of vascular endothelial growth factor (vascular endothelial growth) and matrix metalloproteinase-9 (matrix metalloproteinase-9) in retinal ischemia/reperfusion injury-reperfusion injury in rats. Methods Seventy-eight healthy adult Wistar rats were randomly divided into normal control group (n = 6). 偽 -lipoic acid injection (100mg kg-1) was injected intraperitoneally 3 days before modeling. There were 36 rats in the ischemic group and 36 rats in the ischemic group (36 rats in each group were given intraperitoneal injection of the same amount of normal saline once a day until the death) 3 days before the model was made. The model of retinal ischemia-reperfusion was established by using normal saline anterior chamber perfusion to increase intraocular pressure. The intraocular pressure was maintained at 110 mm Hg.After 60 minutes, the perfusion needle was pulled out. The changes of retinal tissue were observed by HE staining at 6 h, 12 h, 24 h and 48 h, respectively, and the expression of VEGF and MMP-9 in retinal tissue of rats after retinal ischemia-reperfusion injury were detected by immunohistochemical method. Results in the early stage of retinal ischemia reperfusion, the thickness of retinal inner layer edema increased, the number of retinal ganglion cells decreased and the optic nerve fiber layer shrunk in late stage. The change trend of 偽 -lipoic acid treatment group was similar to that of ischemic group. The decrease in the number of cells and the thickness of retina were significantly improved compared with the ischemic group. No significant expression of VEGF and MMP-9 was observed in normal control group (AOD was 0.06 鹵0.04 and 0.05 鹵0.03, respectively). In ischemia group and 偽 -lipoic acid treatment group, the expression of VEGF began to appear 12 hours after reperfusion, and increased gradually. The expression of MMP-9 reached its peak at 48 hours after reperfusion (AOD was 0.52 鹵0.06 and 0.35 鹵0.05, respectively), and the expression of MMP-9 reached its peak at 24 hours after reperfusion (AOD was 0.38 鹵0.06 and 0.29 鹵0.05, respectively). In addition to the expression of VEGF at 6 hours after reperfusion, the expression of VEGF MMP-9 in ischemic group was significantly different from that in normal control group (P0.05), and there was a significant difference between a-lipoic acid treatment group and ischemia-reperfusion group (P0.05). The expression of VEGF and MMP-9 was positively correlated in ischemic group (r = 0.834, P 0.05). Conclusion 偽 -lipoic acid can protect RIRI by inhibiting the expression of VEGF and MMP-9 in RIRI.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R774.1
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 程育宏;申家泉;;基質(zhì)金屬蛋白酶家族及細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子與常見(jiàn)眼內(nèi)惡性腫瘤的關(guān)系[J];國(guó)際眼科雜志;2008年06期
2 曾軍,姜德詠;基質(zhì)金屬蛋白酶及其抑制劑與玻璃體視網(wǎng)膜疾病[J];國(guó)外醫(yī)學(xué).眼科學(xué)分冊(cè);2000年02期
3 李群年,楊文東,高興連;2型糖尿病視網(wǎng)膜病變患者血清VEGF與HbA1C的關(guān)系[J];山西醫(yī)科大學(xué)學(xué)報(bào);2003年06期
4 吳胤瑛,李恩孝;VEGF在惡性腫瘤中的研究進(jìn)展[J];現(xiàn)代腫瘤醫(yī)學(xué);2005年05期
5 毛俊峰,閻元奎,艾育德,劉雙珍,徐慧,田曉峰;大鼠視網(wǎng)膜缺血再灌注后細(xì)胞間粘附分子-1的表達(dá)[J];眼科新進(jìn)展;2004年02期
6 陳建麗,蔡小軍,李琴,張小文;MMP-9、TIMP-1在大鼠視網(wǎng)膜缺血再灌注損傷中的表達(dá)[J];眼科新進(jìn)展;2005年01期
7 凌士奇,丁正平,呂源淑;大鼠視網(wǎng)膜缺血再灌注損傷中的細(xì)胞凋亡[J];眼科;2003年01期
8 游志鵬,姜德詠,唐朝珍,趙宏偉,劉湘平;血管內(nèi)皮生長(zhǎng)因子在視網(wǎng)膜缺血再灌注損傷中的表達(dá)[J];眼外傷職業(yè)眼病雜志.附眼科手術(shù);2004年11期
9 劉燕;夏曉紅;苗智慧;王燕凌;洪麗華;于占久;;α-硫辛酸對(duì)大鼠腎缺血再灌注損傷的影響[J];中國(guó)病理生理雜志;2007年09期
10 羅南萍,黃厚斌,徐軍,楊道理,劉忠倫;VEGF、IFN-γ及NO與糖尿病視網(wǎng)膜病變關(guān)系的研究[J];中國(guó)糖尿病雜志;2001年01期
，本文編號(hào)：2171602
本文鏈接：http://sikaile.net/yixuelunwen/yank/2171602.html
最近更新
教材專著
