【摘要】： 喉鱗狀細(xì)胞癌(laryngeal squamous cell carcinoma,LSCC,簡稱喉癌),在我國東北地區(qū)發(fā)病率遠(yuǎn)高于其他地區(qū),且近年來有明顯增高的趨勢,嚴(yán)重威脅著人類的健康與生命。結(jié)合地域優(yōu)勢,從腫瘤分子遺傳學(xué)角度研究與喉癌發(fā)生、發(fā)展的相關(guān)遺傳因素,尋找和定位喉癌相關(guān)基因,通過關(guān)聯(lián)性研究,分析相關(guān)基因在不同人群中的關(guān)系,對從基因水平上揭示喉癌的發(fā)病機(jī)制有重要的意義。 實(shí)驗(yàn)?zāi)康?探討吉林地區(qū)漢族CSMD1基因單核苷酸多態(tài)性(SNP)與喉鱗狀細(xì)胞癌的關(guān)系。 實(shí)驗(yàn)方法:在CSMD1基因上選取位于第2內(nèi)含子的rs13275581、第3內(nèi)含子的rs7830462、第4內(nèi)含子的rs12675316、第6內(nèi)含子的rs17326768和第10內(nèi)含子的rs17323393等五個SNP位點(diǎn),采用聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性(PCR-RFLP)方法,配合2%瓊脂糖凝膠電泳,對五個SNP位點(diǎn)與喉鱗狀細(xì)胞癌的相關(guān)性進(jìn)行統(tǒng)計(jì)學(xué)檢驗(yàn),探討各個SNP位點(diǎn)與吉林地區(qū)漢族喉鱗狀細(xì)胞癌發(fā)生的關(guān)系。 實(shí)驗(yàn)結(jié)果: 1喉癌組與正常對照組基因頻率經(jīng)Hardy-Weinberg平衡定律檢驗(yàn)(P 0.05),說明實(shí)驗(yàn)所選人群與一般人群無顯著性差異,具有群體代表性,可以代表吉林地區(qū)漢族人群。 2喉癌組與正常對照組之間,CSMD1基因SNP位點(diǎn)rs13275581的基因頻率有顯著性差異,提示CSMD1基因SNP位點(diǎn)rs13275581可能與吉林地區(qū)漢族人群喉癌發(fā)生相關(guān)。 3喉癌組與正常對照組之間,CSMD1基因rs7830462、rs12675316、rs17326768和rs17323393 SNP位點(diǎn)的基因頻率差異均無統(tǒng)計(jì)學(xué)意義。CSMD1基因的這4個SNP位點(diǎn)與吉林地區(qū)漢族人群喉癌的發(fā)生未見關(guān)聯(lián)性。
[Abstract]:The incidence of laryngeal squamous cell carcinoma (laryngeal squamous cell) in Northeast China is much higher than that in other regions, and in recent years, it is a serious threat to human health and life. Based on the regional advantage, the genetic factors related to the occurrence and development of laryngeal carcinoma were studied from the point of view of tumor molecular genetics, and the related genes of laryngeal cancer were searched and located, and the relationship between the related genes in different populations was analyzed. It is of great significance to reveal the pathogenesis of laryngeal carcinoma at the gene level. Objective: to investigate the relationship between single nucleotide polymorphisms (SNP) of CSMD1 gene and laryngeal squamous cell carcinoma (LSCC) in Jilin Han nationality. Methods: five SNP loci, rs13275581, rs7830462, rs12675316, intron 6 and intron 10, were selected from the CSMD1 gene, respectively, in intron 2, intron 3, intron 4, intron 6, and intron 10, respectively, and five SNP loci, rs13275581, rs7830462, rs12675316, intron 6 and intron 10, respectively, were selected. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) combined with 2% agarose gel electrophoresis was used to detect the correlation between five SNP loci and laryngeal squamous cell carcinoma (LSCC). To investigate the relationship between SNP loci and laryngeal squamous cell carcinoma (LSCC) in Jilin Han nationality. Results: 1 the gene frequencies of laryngeal carcinoma group and normal control group were tested by Hardy-Weinberg equilibrium law (P0. 05). The results showed that there was no significant difference between the selected population and the general population. It can represent the Han population in Jilin region. 2 there was significant difference in the frequency of rs13275581 gene in SNP locus of CSMD1 gene between laryngeal carcinoma group and normal control group. The results suggest that the SNP rs13275581 of CSMD1 gene may be associated with laryngeal carcinoma in the Han nationality of Jilin region. 3 there is no significant difference in the frequency of rs7830462rs12675316rs17326768 and rs17323393 SNP locus between the laryngeal cancer group and the normal control group. The four SNP loci of CSMD1 gene were not associated with laryngeal cancer in Jilin Han population.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.65

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 孫海波;CSMD1基因和BRG1基因與喉鱗狀細(xì)胞癌發(fā)生的相關(guān)性研究[D];吉林大學(xué);2012年

，

本文編號：2163757