【摘要】： 目的：探討OSAHS患者白細胞粘附分子CD15、CD11c的表達、ROS產(chǎn)生和它們之間的相關(guān)性,以及nCPAP治療對粘附分子CD15、CD11c及其ROS水平的影響。 方法：按入院時間選擇30例多導(dǎo)睡眠監(jiān)測確診的OSAHS患者作為實驗組,男性28例,女性2例,年齡25-65歲(平均43.90±10.69歲),并將年齡和BMI匹配的12例健康志愿者作為對照組。所有參與者均簽署知情同意書。以清晨空腹靜脈血(5ml)作為血液標本,比較實驗組和對照組單核細胞、淋巴細胞CD15、CD11c的表達、ROS產(chǎn)生和它們之間的差異和相關(guān)性,隨機對實驗組中的10例行nCPAP治療,并分析nCPAP治療前后各項指標的變化。 結(jié)果： 1.單核細胞和淋巴細胞粘附分子CD15、CD11c的表達。OSAHS組淋巴細胞CD15的表達高于正常組(t=3.048,P＜0.05)；OSAHS組淋巴細胞CD11c的表達高于正常組(t=2.658,P＜0.05)；OSAHS組單核細胞CD15的表達高于正常組(t=2.726,P＜0.05);OSAHS組單核細胞CD11c的表達高于正常組(t=1.611,P0.05)。 2.單核細胞中ROS產(chǎn)生的檢測。OSAHS組單核細胞基礎(chǔ)的ROS的產(chǎn)生高于正常組(t=7.859,P＜0.05),加入Rosup刺激物后,OSAHS組在單核細胞ROS的產(chǎn)生高于基礎(chǔ)ROS的產(chǎn)生,而且高于對照組(t=12.038,P＜0.05) 3.淋巴細胞中ROS產(chǎn)生的檢測。OSAHS組淋巴細胞基礎(chǔ)ROS的產(chǎn)生高于正常組(t=16.250,P＜0.05),加入Rosup刺激物后,OSAHS組單核細胞ROS的產(chǎn)生高于基礎(chǔ)ROS的產(chǎn)生,而且高于對照組(t=7.436,P＜0.05)。 4. nCPAP治療的影響。nCPAP治療后淋巴細胞CD15的表達較OSAHS組下調(diào)(t=4.257,P＜0.05),CD11c的表達較OSAHS組下調(diào)(t=3.997,P0.05),單核細胞CD15的表達較OSAHS組下調(diào)(t=2.593,P0.05),單核細胞CD11c的表達較OSAHS組下調(diào)(t=2.736,P＜0.05)。nCPAP治療后淋巴細胞基礎(chǔ)ROS的產(chǎn)生較OSAHS組下調(diào)(t=4.701,P0.05),單核細胞基礎(chǔ)ROS的產(chǎn)生較OSAHS組下調(diào)(t=5.739,P＜0.05),Rosup刺激組淋巴細胞ROS的產(chǎn)生下調(diào)(t=6.008,P0.05)。Rosup刺激組單核細胞ROS的產(chǎn)生也下調(diào)(t=6.751,P0.05)。 5.ROS的產(chǎn)生與CD15、CD11c兩種粘附分子的相關(guān)性。淋巴細胞中CD15與基礎(chǔ)活性氧及Rosup刺激組是相關(guān)的(r=0.888,P＜0.01；r=0.799,P＜0.01),淋巴細胞中CD11c與基礎(chǔ)活性氧及Rosup刺激組是相關(guān)的(r=0.901,P＜0.01；r=0。849,P＜0.01),單核細胞中CD15與基礎(chǔ)活性氧及Rosup刺激組是相關(guān)的(r=0.766,P0.01；r=0.663,P0.01),單核細胞中CD11c與基礎(chǔ)活性氧及Rosup刺激組是相關(guān)的(r=0.812,P0.01；r=0.667,P＜0.01)。 結(jié)論： 1. OSAHS患者淋巴細胞、單核細胞CD15、CD11c兩種粘附分子的表達增強。 2. OSAHS患者淋巴細胞、單核細胞ROS的產(chǎn)生增強。 3. nCPAP治療可以下調(diào)淋巴細胞、單核細胞粘附分子CD15、CD11的表達、減低ROS的產(chǎn)生。 4.ROS的產(chǎn)生與CD15、CD11c兩種粘附分子的增強表達相關(guān)。
[Abstract]:Objective: to investigate the expression of CD15pCD11c in leukocyte of patients with OSAHS and its correlation with Ros, and to investigate the effect of nCPAP therapy on CD15 CD11c and its ROS level. Methods: 30 patients with OSAHS diagnosed by polysomnography were selected as experimental group (28 males and 2 females, aged 25-65 years, mean 43.90 鹵10.69 years), and 12 healthy volunteers matched with BMI were used as control group. All participants sign informed consent. Early morning fasting venous blood (5ml) was used as blood sample to compare the expression of CD15 ~ + CD11c in monocytes and lymphocytes and the difference and correlation between them. 10 cases of experimental group were treated with nCPAP randomly. The changes of indexes before and after nCPAP treatment were analyzed. Results: 1. The expression of CD15 in lymphocytes in OSAHS group was higher than that in normal group (t 3.048g P < 0. 05). The expression of CD11c in OSAHS group was higher than that in normal group (t 2. 658% P < 0. 05). The expression of CD15 in monocytes in OSAHS group was higher than that in normal group (t = 2.726, P < 0. 05). The expression of CD11c in monocytes in OSAHS group was higher than that in normal group (t 1. 611). 2. Detection of ROS production in monocytes. The ROS production of monocytes in the OSAHS group was higher than that in the normal group (t = 7.859, P < 0. 05), and the production of ROS in monocytes was higher than that in the basic ROS group after the addition of Rosup stimulator, and was higher than that in the control group (t = 12. 038, P < 0. 05). Detection of ROS production in lymphocytes. The production of basic ROS in lymphocytes in OSAHS group was higher than that in normal group (t = 16.250, P < 0. 05). The ROS production of monocytes in OSAHS group was higher than that in basic ROS group after adding Rosup stimulator. The expression of CD11c in nCPAP group was lower than that in OSAHS group (t 4.257, P < 0. 05), the expression of CD15 in monocytes was lower than that in OSAHS group (P0.05), the expression of CD15 in monocytes was lower than that in OSAHS group (t2. 593P 0.05), and the expression of CD11c in monocytes was lower than that in OSAHS group (P < 0. 05), and the expression of CD15 in monocytes was lower than that in OSAHS group (P < 0. 05), and the expression of CD15 in monocytes was lower than that in OSAHS group (P < 0. 05). The expression of CD11c was lower than that of OSAHS group (tnCPAP). After treatment with nCPAP, the production of basic ROS in lymphocytes was lower than that in OSAHS group (p0.05), and the production of basic ROS in monocytes was lower than that in OSAHS group (t 5.739U P < 0. 05). The ROS production of lymphocytes in rosup-stimulated group was down-regulated (t6.008p0.05). The production of ROS was also down-regulated (P 0.05). The production of 5.ROS was correlated with CD15 and CD11c. CD15 in lymphocytes was correlated with basal reactive oxygen species (Ros) and Rosup stimulation group (P < 0.01), and CD11c in lymphocytes was correlated with basal Ros and Rosup (r 0.901U P < 0.01). CD15 in monocytes was correlated with basal reactive oxygen species (Ros) and Rosup stimulation (rP0. 766G, P 0. 01). CD11c in monocytes was correlated with basal reactive oxygen species (Ros) and Rosup (P < 0. 01). Conclusion: 1. The expression of CD15 and CD11c in lymphocytes and monocytes of OSAHS patients was increased. 2. 2. In patients with OSAHS, the production of monocyte ROS was increased. 3. The expression of monocyte adhesion molecule CD15, CD11, was down-regulated by nCPAP treatment. To reduce the production of ROS. The production of 4.ROS is related to the enhanced expression of CD15, CD11c and CD11c.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R766

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