本文選題：恒定性外斜視 + 眼外肌�。� 參考：《青島大學(xué)》2011年碩士論文

【摘要】：目的：觀察恒定性外斜視弱側(cè)眼外肌形態(tài)改變及MyoD和Pax7表達(dá)變化,探討MyoD、Pax7在恒定性外斜視發(fā)生、發(fā)展中的作用。 方法：收集2009年12月至2010年9月在青島大學(xué)醫(yī)學(xué)院附屬醫(yī)院眼科住院手術(shù)的恒定性外斜視患者24例作為實驗組,分為兒童外斜視組、成人外斜視組各12例,另選同期角膜移植供體的眼外肌10例作為成人對照組。實驗組手術(shù)截取的內(nèi)直肌及對照組的內(nèi)直肌固定切片后行HE染色,在光鏡下觀察肌纖維的大小、形態(tài)、數(shù)量、走形方向等,統(tǒng)計肌纖維數(shù)量及肌細(xì)胞橫截面積。免疫組化法測量MyoD和Pax7的平均光密度值。 結(jié)果：恒定性外斜視弱側(cè)眼外肌肌纖維數(shù)量減少,大小及形態(tài)不一,走形方向不一致,細(xì)胞間質(zhì)成分增多。成人外斜視組肌纖維數(shù)量為18.77±2.91個,成人對照組為32.53±4.75個,兩者差異具有統(tǒng)計學(xué)意義(P0.01)。而兒童外斜視組為20.81±4.27個,與成人外斜視組比較,差異無統(tǒng)計學(xué)意義(P0.05)。肌纖維橫截面積：成人外斜視組為261.76±35.68μm2,成人對照組708.9±62.9μm2,兩者差異具有統(tǒng)計學(xué)意義(P0.01)。而兒童外斜視組平均肌纖維橫截面積為282.46±25.22μm2,與成人外斜視組比較,差異無統(tǒng)計學(xué)意義(P0.05)。成人外斜視組MyoD的平均光密度值(0.011±0.006)、Pax7的值(0.013±0.005)均較成人對照組(0.028±0.0008,0.027±0.002)明顯減少(P0.01)。兒童外斜視組MyoD值(0.021±0.024)、Pax7的值(0.014±0.004)分別與成人外斜視組比較,差異均無統(tǒng)計學(xué)意義(P0.05)。 結(jié)論：恒定性外斜視弱側(cè)眼外肌肌纖維發(fā)生了萎縮、減少為主的形態(tài)學(xué)改變,以及MyoD, Pax7的表達(dá)下降,這些改變可能與共同性外斜視斜視的發(fā)生及發(fā)展有關(guān)。
[Abstract]:Aim: to observe the morphological changes of weak lateral extraocular muscles and the expression of MyoD and Pax7 in constant exotropia, and to explore the role of MyoD Pax7 in the occurrence and development of constant exotropia. Methods: from December 2009 to September 2010, 24 patients with constant exotropia who were operated on in the affiliated Hospital of Qingdao University Medical College as experimental group were divided into two groups: child exotropia group (n = 12), adult exotropia group (n = 12) and adult exotropia group (n = 12). Another 10 cases of corneal transplantation donor were selected as the adult control group. The internal rectus muscle of the experimental group and the internal rectus muscle of the control group were fixed by HE staining. The size, shape, quantity and direction of the muscle fibers were observed under light microscope, and the number of muscle fibers and the cross sectional area of muscle cells were counted. The mean optical density of MyoD and Pax7 was measured by immunohistochemical method. Results: the number of muscle fibers in the weak lateral extraocular muscle of constant exotropia was decreased, the size and shape of the muscle fibers were different, the direction of deviation was different, and the content of the interstitial cells was increased. The number of muscle fibers in adult exotropia group was 18.77 鹵2.91, and that in adult control group was 32.53 鹵4.75. The difference was statistically significant (P0.01). The number of children with exotropia was 20.81 鹵4.27, which was not significantly different from that of adult exotropia (P0.05). The cross sectional area of muscle fiber was 261.76 鹵35.68 渭 m2 in adult exotropia group and 708.9 鹵62.9 渭 m2 in adult control group. The difference was statistically significant (P0.01). The mean cross sectional area of muscle fiber in children with exotropia was 282.46 鹵25.22 渭 m ~ (2), which was not significantly different from that in adult exotropia group (P0.05). The mean optical density of MyoD in adult exotropia group (0.011 鹵0.006) was (0.013 鹵0.005) lower than that in adult control group (0.028 鹵0.0008 鹵0.027 鹵0.002). The values of MyoD (0.021 鹵0.024) and Pax7 (0.014 鹵0.004) in children with exotropia were not significantly different from those in adult exotropia (P0.05). Conclusion: the weak lateral extraocular muscle fibers of the concomitant exotropia may be related to the occurrence and development of concomitant exotropia with atrophy, decreased morphologic changes, and decreased expression of MyoD, Pax7, which may be related to the occurrence and development of concomitant exotropia.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R779.6

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 邱芳芳;共同性斜視的病理解剖學(xué)研究進(jìn)展[J];福建醫(yī)藥雜志;2002年02期

2 欒瑛;王淑霞;劉斌;周博研;周麗娜;;共同性斜視眼外肌病理改變的研究[J];國際眼科雜志;2008年01期

3 李月平;MRI在非共同性斜視中的研究應(yīng)用[J];國外醫(yī)學(xué).眼科學(xué)分冊;2003年06期

4 陳若澤，梁瑩，甘小敏，莫肖敏，覃作棟，，覃烽，吳鐵蘭;共同性斜視眼肌的組織學(xué)研究[J];廣西科學(xué);1996年03期

5 劉秉慈，許增祿;細(xì)胞外間質(zhì)[J];生理科學(xué)進(jìn)展;1996年03期

6 陳淑香,陳琳,李誠,趙燕玲;寧夏平羅縣學(xué)齡前兒童斜視弱視普查[J];眼科新進(jìn)展;2003年05期

7 陳麗英,徐國興,梁平,陳蓮云;共同性外斜視眼肌的超微病理研究[J];眼科;2000年01期

8 馬少青;徐國興;吳松一;;共同性斜視眼外肌肌酶組織化學(xué)研究[J];海峽科學(xué);2007年12期

9 徐國興;吳松一;馬少青;;肌動蛋白和肌球蛋白在共同性斜視眼外肌的表達(dá)研究[J];海峽科學(xué);2007年12期

10 劉桂香;趙會元;孔慶蘭;胡聰;;共同性外斜視ARIX基因多態(tài)性研究[J];眼科研究;2007年01期

相關(guān)博士學(xué)位論文 前1條

1 李月平;共同性外斜視眼外肌病理學(xué)和MyoD、myogenin表達(dá)的研究[D];天津醫(yī)科大學(xué);2009年

相關(guān)碩士學(xué)位論文 前1條

1 王雪;麻痹性上斜視功能亢進(jìn)的下斜肌形態(tài)結(jié)構(gòu)改變及活性衛(wèi)星細(xì)胞表達(dá)的研究[D];中南大學(xué);2007年

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