本文選題：喉鱗癌 + Bcl-2　； 參考：《南華大學》2014年碩士論文

【摘要】：目的：通過檢測Bcl-2,E-cadherin,N-cadherin蛋白在喉鱗癌中的表達水平，探討B(tài)cl-2,E-cadherin,N-cadherin蛋白三者與喉鱗癌發(fā)生發(fā)展、臨床病理特征的關系,為進一步闡明喉鱗癌的發(fā)病機制，診斷指標、評估預后提供理論和實驗依據(jù)。材料與方法：47例人喉鱗癌標本由湖南省腫瘤醫(yī)院病理科提供，26例聲帶息肉標本來自南華大學病理教研室，40例正常喉粘膜標本來源南華大學司法鑒定中心。石蠟標本每個切5張白片，分別進行HE染色、免疫組化、陰性對照。采用SP法檢測喉鱗癌及非喉癌組織（非典型增生、聲帶息肉及正常喉粘膜）中Bcl-2,E-cadherin,N-cadherin蛋白的表達，以PBS代替一抗作為陰性對照。 結果： 1、Bcl-2蛋白陽性表達率在喉鱗癌、非典型增生、聲帶息肉以及正常喉粘膜中分別為61.7%（29/47）、33.3%(11/33)、19.2%（5/26）、0（0/40）。Bcl-2蛋白在喉鱗癌中的表達率遠高于在癌旁、息肉及正常喉粘膜的組織。喉鱗癌中Bcl-2蛋白與喉鱗癌的病理組織學分級、臨床分期、淋巴結轉移、浸潤深度等相關（P＜0.05），與年齡、性別無關（P0.05）。 2、喉鱗癌、非典型增生、聲帶息肉及正常喉粘膜中E-cadherin蛋白陽性表達率分別為40.4%（19/47）、66.7%（22/33）、84.7%（22/26）、100%（40/40）。E-cadherin蛋白在喉鱗癌中的表達率遠低于在癌旁、息肉及正常的喉粘膜組織。E-cadherin蛋白的陽性表達和喉鱗癌的病理組織學分級、臨床分期、淋巴結轉移、浸潤深度等密切相關（P＜0.05），與年齡、性別無關（P0.05）。 3、喉鱗癌、非典型增生、聲帶息肉及正常喉粘膜中N-cadherin蛋白陽性表達率分別為70.2%（33/47）、42.4%（14/33）、11.5%（3/26）、0（0/40）。N-cadherin蛋白的陽性表達與喉鱗癌的病理學組織分級、臨床分期、淋巴結轉移、浸潤深度等相關（P＜0.05），與年齡、性別無關（P0.05）。 4、喉鱗癌中，Bcl-2與E-cadherin蛋白的表達呈負相關(r=-0.421,P=0.003)，與N-cadherin蛋白的表達呈正相關(r=0.635, P=0.000)， E-cadherin蛋白與N-cadherin蛋白的表達呈負相關(r=-0.411, P=0.004)。 5、生存分析:對有生存資料的47例喉鱗癌患者進行生存分析，我們發(fā)現(xiàn)Bcl-2蛋白強陽性表達者生存期明顯低于陰性表達者，E-cadherin陰性表達者、N-cadherin蛋白陽性表達者其生存期明顯縮短。 結論： 1、喉鱗癌中Bcl-2，N-cadherin蛋白表達上調，E-cadherin蛋白表達下調。 2、Bcl-2，E-cadherin和N-cadherin蛋白的表達與喉鱗癌的臨床分期、病理學組織分級、淋巴結癌轉移及生存期密切相關。 3、聯(lián)合檢測Bcl-2，E-cadherin，，N-cadherin蛋白在喉鱗癌中表達，對預測喉鱗癌的侵襲轉移、判斷預后具有重要意義。
[Abstract]:Objective: to investigate the relationship between Bcl-2E-cadherin protein and carcinogenesis and clinicopathological features of laryngeal squamous cell carcinoma (LSCC) by detecting the expression of Bcl-2E-cadherin protein in laryngeal squamous cell carcinoma (LSCC), and to elucidate the pathogenesis of laryngeal squamous cell carcinoma (LSCC). Evaluation of prognosis provides theoretical and experimental evidence. Materials and methods Forty-seven human laryngeal squamous cell carcinoma specimens were collected from the Department of Pathology of Hunan Cancer Hospital. 26 vocal cord polyp specimens were collected from 40 normal laryngeal mucosa specimens from the South China University Forensic Identification Center. Paraffin wax specimens were divided into 5 white slices and stained with HE, immunohistochemistry and negative control respectively. The expression of Bcl-2 E-cadherin N-cadherin protein in laryngeal squamous cell carcinoma (LSCC) and non-laryngeal carcinoma (atypical hyperplasia, vocal cord polyp and normal laryngeal mucosa) was detected by SP method. Results: 1 the positive expression rate of Bcl-2 protein in laryngeal squamous cell carcinoma, atypical hyperplasia, vocal cord polyp and normal laryngeal mucosa was 61.7% (29 / 47) or 33.3% (11 / 33) or 19.2% (5 / 26) / 0 (0 / 40) .Bcl-2 protein expression in laryngeal squamous cell carcinoma was significantly higher than that in adjacent laryngeal squamous cell carcinoma, polyp and normal laryngeal mucosa. Bcl-2 protein in laryngeal squamous cell carcinoma was correlated with histological grade, clinical stage, lymph node metastasis and depth of invasion (P < 0.05), but not with age and sex (P0.05). The positive expression rates of E-cadherin protein in vocal cord polyps and normal laryngeal mucosa were 40.4% (19 / 47), 66.7% (22 / 33), 84.7% (22 / 26) and 100% (40 / 40). Clinical stage, lymph node metastasis, depth of invasion were closely related (P < 0.05), not related to age and sex (P0.05), laryngeal squamous cell carcinoma (LSCC), atypical hyperplasia, and so on. The positive expression rates of N-cadherin protein in vocal cord polyps and normal laryngeal mucosa were 70.2% (33 / 47), 42.4% (14 / 33) and 11.5% (3 / 26), respectively. The positive expression of N-cadherin protein was correlated with the pathological grade, clinical stage, lymph node metastasis, depth of invasion of laryngeal squamous cell carcinoma (P < 0.05). The expression of Bcl-2 and E-cadherin in laryngeal squamous cell carcinoma was negatively correlated with the expression of E-cadherin protein (r-0.421, P0.003), and positively correlated with the expression of N-cadherin protein (r-0.635, P0. 000), and the expression of E-cadherin protein was negatively correlated with the expression of N-cadherin protein (r-0.411, P0. 004). 5. Survival analysis: a positive correlation was found between the expression of E-cadherin protein and the expression of N-cadherin protein (r-0.411, P0. 004). Data of 47 patients with laryngeal squamous cell carcinoma were analyzed. We found that the survival time of patients with strong positive expression of Bcl-2 protein was significantly shorter than that of those with negative expression of E-cadherin protein and positive expression of N-cadherin protein. Conclusion: 1, the expression of Bcl-2mN-cadherin protein up-regulates the expression of E-cadherin protein in laryngeal squamous cell carcinoma, and the expression of E-cadherin protein and N-cadherin protein in laryngeal squamous cell carcinoma is correlated with the clinical stage and pathological grade of laryngeal squamous cell carcinoma. The expression of Bcl-2E-cadherin N-cadherin protein in laryngeal squamous cell carcinoma (LSCC) was closely related to its metastasis and survival time. 3. The expression of Bcl-2E-cadherinN-cadherin protein in laryngeal squamous cell carcinoma was significant in predicting invasion and metastasis of laryngeal squamous cell carcinoma and judging prognosis.
【學位授予單位】：南華大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R739.65

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