本文選題：阻塞性睡眠呼吸暫停低通氣綜合征 + 慢性間歇低氧��； 參考：《中南大學(xué)》2014年碩士論文

【摘要】：目的：通過(guò)模擬OSAHS病理生理改變-慢性間歇性低氧(CIH)建立大鼠模型,探究CIH對(duì)大鼠頦舌肌的影響；并用NGF干預(yù)CIH大鼠,探討NGF對(duì)CIH大鼠頦舌肌的作用及其機(jī)制,以期為治療OSAHS提供新思路。 方法：通過(guò)自制的間歇性低氧倉(cāng)建立CIH大鼠模型。28只SD大鼠隨機(jī)分為正常對(duì)照組(Control組)、CIH組、CIH+NGF組、CIH+抗NGF組,每組7只。正常對(duì)照組大鼠置于空氣循環(huán)的艙內(nèi),其余各組大鼠置于間歇性低氧艙內(nèi),每日8h,共5周；正常對(duì)照組及CIH組大鼠每日均給予生理鹽水lml/Kg尾靜脈注射,CIH+NGF組及CIH+抗NGF組每日分別給予1μg/Kg NGF、2μg/Kg抗NGF尾靜脈注射。5周后麻醉大鼠,分離大鼠頦舌肌舌下神經(jīng)及頦舌肌,頦舌肌一端離斷連接張力換能器,單刺激神經(jīng)干記錄單刺激頦舌肌最大收縮張力,然后給予連續(xù)刺激引起肌肉強(qiáng)直收縮,誘導(dǎo)頦舌肌疲勞,并記錄頦舌肌強(qiáng)直收縮曲線。電生理檢測(cè)完畢,取出頦舌肌組織,一部分置于10%福爾馬林溶液,石蠟包埋后做HE染色測(cè)量大鼠頦舌肌橫截面積、免疫組化分析NGF表達(dá)情況；另一部分保存于-70℃冰箱中,TBA法測(cè)量大鼠頦舌肌MDA水平,羥胺法測(cè)SOD水平,TNB法測(cè)大鼠頦舌肌乙酰膽堿酯酶活性。 結(jié)果：實(shí)驗(yàn)前各組大鼠體重?zé)o顯著性差異(P0.05),實(shí)驗(yàn)后各組大鼠體重差異亦無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。各組大鼠頦舌肌單刺激最大收縮張力差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。與對(duì)照組比較,CIH組、CIH+NGF組、及CIH+抗NGF組大鼠頦舌肌50%收縮時(shí)程均降低(P0.01), CIH組及CIH+抗NGF組較CIH+NGF組降低更加明顯(P0.05),CIH組和CIH+抗NGF組無(wú)顯著性差異(P0.05)。與對(duì)照組比較,CIH組、CIH+NGF組、CIH+抗NGF組大鼠頦舌肌SOD降低(P0.05), MDA水平均升高(P0.05), AChE活性減低(P0.05); CIH+NGF組大鼠頦舌肌SOD水平及AGhE活性較CIH組高,而MDA水平降低(P0.05), CIH+抗NGF組與CIH組比較無(wú)顯著性差異(P0.05)。大鼠頦舌肌組織橫切片HE染色示CIH組、CIH+NGF組、CIH+抗NGF組肌纖維橫截面積均小于正常對(duì)照組(P0.05),CIH組、CIH+NGF組、CIH+抗NGF組各組之間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。正常對(duì)照組大鼠頦舌肌組織可見(jiàn)NGF表達(dá)。CIH組、CIH+NGF組、CIH+抗NGF組NGF表達(dá)均低于正常對(duì)照組(P0.05),CIH+NGF組較CIH組、CIH+抗NGF組NGF表達(dá)高(P0.05)。 結(jié)論：1.CIH可以導(dǎo)致大鼠頦舌肌結(jié)構(gòu)與神經(jīng)活性的改變,增加頦舌肌的易疲勞性。 2.NGF可部分改善CIH大鼠頦舌肌的易疲勞性,可能是通過(guò)抗氧化機(jī)制修復(fù)頦舌肌結(jié)構(gòu)及神經(jīng)活性的損傷。
[Abstract]:Objective : To explore the effect of CIH on the genioglossus of the rats by simulating the pathological changes of obstructive sleep - chronic intermittent hypoxia ( CIH ) .
Using NGF to interfere with CIH rats , the effect and mechanism of NGF on the genioglossus of CIH rats were investigated .

Methods : CIH rat model was established by self - made intermittent hypoxia . 28 SD rats were randomly divided into control group ( control group ) , CIH group , CIH + NGF group , CIH + anti - NGF group .
In normal control group and CIH group , normal saline lml / Kg tail vein injection , CIH + NGF group and CIH + anti - NGF group were given 1 渭g / kg NGF and 2 渭g / Kg anti - NGF group every day .
Another part was stored in the refrigerator at -70 鈩，

本文編號(hào)：2096457