本文選題：慢性鼻-鼻竇炎 + 黏蛋白��； 參考：《中南大學》2011年碩士論文

【摘要】：目的：檢驗2種黏蛋白(MUC5AC, MUC5B)在慢性鼻-鼻竇炎患者術(shù)前、術(shù)后及正常鼻黏膜中的表達情況,探討?zhàn)さ鞍讓β员?鼻竇炎鼻腔黏膜超分泌的影響及臨床意義。 方法：收集行鼻內(nèi)鏡手術(shù)治療慢性鼻-鼻竇炎患者術(shù)前鼻腔上頜竇口黏膜(20例),術(shù)后6個月上頜竇口黏膜(20例),正常下鼻甲上緣黏膜(5例)。免疫組織化學(ABC)染色法檢測及定位慢性鼻-鼻竇炎患者鼻黏膜中黏蛋白的表達。 結(jié)果：通過免疫組織化學染色,觀察黏蛋白MUC5AC, MUC5B在鼻黏膜組織中分布情況。結(jié)果顯示黏蛋白在慢性鼻-鼻竇炎患者鼻黏膜中的表達主要集中在杯狀細胞的胞漿(MUC5AC)、黏膜下腺體胞漿(MUC5B)中。術(shù)前組的鼻黏膜陽性表達高于術(shù)后組,兩者差異有統(tǒng)計學意義(p0.05)。術(shù)后組及對照組的鼻黏膜陽性表達相接近,兩者差異無統(tǒng)計學意義(p0.05)。術(shù)前組的鼻黏膜陽性表達高于對照組,兩者差異有統(tǒng)計學意義(p0.05) 結(jié)論：1.MUC5AC、MUC5B在鼻黏膜中均有表達,其中MUC5AC表達主要集中在杯狀細胞的胞漿,MUC5B表達主要集中在黏膜下腺體細胞胞漿。 2.免疫組化結(jié)果顯示MUC5AC、MUC5B在慢性鼻-鼻竇炎鼻黏膜術(shù)前組中表達較術(shù)后組和對照組高,MUC5AC、MUC5B在慢性鼻-鼻竇炎黏液中呈高水平分泌。術(shù)后6個月時,患者鼻腔黏膜基本完成上皮化,鼻腔未見明顯膿性分泌物的情況下,MUC5AC、MUC5B在慢性鼻-鼻竇炎鼻黏膜中的表達下調(diào)。
[Abstract]:Objective: to examine the expression of two kinds of mucin (MUC5AC, MUC5B) in the nasal mucosa of patients with chronic rhinosinusitis before and after operation, and to investigate the effect of MUC5AC on the hypersecretion of nasal mucosa in patients with chronic rhinosinusitis and its clinical significance. Methods: 20 patients with chronic rhinosinusitis were treated with endoscopic sinus surgery (20 cases), 20 cases with oral mucosa of maxillary sinus (20 cases) 6 months after operation and 5 cases with normal superior inferior turbinate mucosa (5 cases). The expression of mucin in nasal mucosa of patients with chronic rhinosinusitis was detected by immunohistochemical staining. Results: the distribution of mucin MUC5ACand MUC5B in nasal mucosa was observed by immunohistochemical staining. The results showed that the expression of mucin in nasal mucosa of patients with chronic rhinosinusitis was mainly in the cytoplasm of goblet cells (MUC5AC) and the cytoplasm of submucosal glands (MUC5B). The positive expression of nasal mucosa in preoperative group was higher than that in postoperative group (p 0.05). The positive expression of nasal mucosa in the postoperative group and the control group was similar, and there was no significant difference between the two groups (p 0.05). The positive expression of MUC5ACC5B in the nasal mucosa of the preoperative group was significantly higher than that of the control group (p0.05). Conclusion the positive expression of MUC5ACC5B in the nasal mucosa was significantly higher than that in the control group (p0.05). The expression of MUC5AC was mainly concentrated in the cytoplasm of goblet cells. The results of immunohistochemistry showed that MUC5ACmMUC5B was higher in mucus of chronic rhinosinusitis than that in postoperative group and control group, and the expression of MUC5B in mucus of chronic rhinosinusitis was higher than that of control group. At 6 months after operation, the mucosal epithelization of nasal cavity was basically completed, and the expression of MUC5AC MUC5B was down-regulated in the nasal mucosa of chronic rhinosinusitis without obvious purulent secretion.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R765.9

【參考文獻】

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